POLR2D antibody
Principal name
POLR2D antibody
Alternative names for POLR2D antibody
RNA polymerase II subunit B4, RPB16, DNA-directed RNA polymerase II subunit RPB4, DNA-directed RNA polymerase II subunit D, RNA polymerase II 16 kDa subunit
Ncbi ID
9606, NP_004796, NP_004796.1, NP_081278.1, NM_027101, NM_027002, NM_004805
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB)
Background of POLR2D antibody
- Kit Component:
- - KN313600G1, Polr2d gRNA vector 1 in pCas-Guide vector
- KN313600G2, Polr2d gRNA vector 2 in pCas-Guide vector
- KN313600D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Khazak V., et al. (1998). Mol. Cell. Biol. 18:1935-1945
Western Blot analysis of POLR2D expression in transfected 293T cell line by POLR2D monoclonal antibody (M01), clone 1E4-A5.Lane 1: POLR2D transfected lysate(16.3 KDa).Lane 2: Non-transfected lysate.
Predicted band size: 16 kDa . Positive control: Heart tissue lysate. Recommended dilution: 1/500-2000. (Gel: 12%SDS-PAGE Lysate: 40 ug Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute)
Western blot analysis of extracts of various cell lines, using POLR2D antibody.
Immunohistochemistry of paraffin-embedded human esophageal cancer using POLR2D antibody at dilution of 1:100 (400x lens).
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA322707 | POLR2D antibody |
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| Anti-POLR2D Rabbit Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA332448 | POLR2D antibody |
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| Rabbit anti-POLR2D Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt |
0.1 mg /
€335.00
|
||
| OriGene Technologies, Inc. | |||||||
 +1 additional image |
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Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP301116 | POLR2D |
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| Recombinant protein of human polymerase (RNA) II (DNA directed) polypeptide D (POLR2D) | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR200351L2V | Lenti ORF particles, Polr2d (GFP-tagged) - Mouse polymerase (RNA) II (DNA directed) polypeptide D (Polr2d), transcript variant 2, 200 uL, >10^7 TU/mL |
|
 |
200 µl /
€930.00
|
|
| OriGene Technologies, Inc. | ||
| MR200351L1V | Lenti ORF particles, Polr2d (Myc-DDK-tagged) - Mouse polymerase (RNA) II (DNA directed) polypeptide D (Polr2d), transcript variant 2, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€830.00
|
|
| OriGene Technologies, Inc. | ||