POLR2J2 antibody
Principal name
POLR2J2 antibody
Alternative names for POLR2J2 antibody
RNA polymerase II subunit B11-b1, RPB11b1, RPB11, DNA-directed RNA polymerase II subunit RPB11-b1, DNA-directed RNA polymerase II subunit J2
SwissProt ID
Q9GZM3 (Human)
Ncbi ID
9606, NP_116581, NP_001091084.2, NP_116581.3, NM_032959, NM_001097615
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Flow Cytometry (F), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P)
Background of POLR2J2 antibody
- Kit Component:
- - KN220730G1, POLR2J3 gRNA vector 1 in pCas-Guide vector
- KN220730G2, POLR2J3 gRNA vector 2 in pCas-Guide vector
- KN220730D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Palangat M., et al. (2004) J Mol Biol. 341:429-442Uivari A., et al. (2006) Nat Struct Mol Biol. 13: 49-54.
Western Blot analysis of POLR2J2 expression in transfected 293T cell line by POLR2J2 monoclonal antibody (M01), clone 1B2.Lane 1: POLR2J2 transfected lysate(12.7 KDa).Lane 2: Non-transfected lysate.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY POLR2J2 (RC224755, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-POLR2J2.
Flow cytometric Analysis of Hela cells, using anti-POLR2J2 antibody(TA502406),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
Flow cytometric Analysis of Jurkat cells, using anti-POLR2J2 antibody(TA502406),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-POLR2J2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502454)
Anti-POLR2J2 mouse monoclonal antibody (TA502454) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY POLR2J2(RC224755).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY POLR2J2 (RC224755, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-POLR2J2.
HEK293T cells transfected with either RC224755 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-POLR2J2 antibody(TA502454), and then analyzed by flow cytometry.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY POLR2J2 (RC224755, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-POLR2J2.
HEK293T cells transfected with either RC224755 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-POLR2J2 antibody(TA502455), and then analyzed by flow cytometry.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY POLR2J2 (RC224755, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-POLR2J2.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-POLR2J2 monoclonal antibody.
Western blot analysis of lysate from MCF-7 cell line,using POLR2J3 Antibody (N-term)(Cat. #TA328157).TA328157 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody.Lysate at 35ug per lane.
- Quick-Filters:
- Highly Validated (1)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA502454 | POLR2J2 antibody |
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| POLR2J2 mouse monoclonal antibody, clone OTI3G4 (formerly 3G4) | |||||||
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Mouse | IgG2a | OTI3G4 | Purified | Hu | F, ICC/IF, P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
 +3 additional images |
Highly validated antibody!
|
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| TA328157 | POLR2J2 antibody |
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| Rabbit polyclonal anti-POLR2J3 antibody (N-term) | |||||||
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Rabbit | RB45111 | Purified | Hu | WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA502406 | POLR2J2 antibody |
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| POLR2J2 mouse monoclonal antibody, clone OTI1C6 (formerly 1C6) | |||||||
 |
Mouse | IgG1 | OTI1C6 | Purified | Hu | F, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
|
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| TA502455 | POLR2J2 antibody |
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| POLR2J2 mouse monoclonal antibody, clone OTI6E9 (formerly 6E9) | |||||||
 |
Mouse | IgG2a | OTI6E9 | Purified | Hu | F, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|
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| TA502562 | POLR2J2 antibody |
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| POLR2J2 mouse monoclonal antibody, clone OTI8F10 (formerly 8F10) | |||||||
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Mouse | IgG2a | OTI8F10 | Purified | Can, Hu, Mky, Rt | WB |
0.1 ml /
€379.00
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|
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Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP324755 | POLR2J2 |
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| Recombinant protein of human polymerase (RNA) II (DNA directed) polypeptide J2 (POLR2J2) | |||||
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Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
| TP760696 | POLR2J2 (N-term HIS tag) |
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| Purified recombinant protein of Human polymerase (RNA) II (DNA directed) polypeptide J2 (POLR2J2), with N-terminal HIS tag, expressed in E.Coli, 50ug | |||||
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Human | > 80 % Preparation: . Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
E. coli |
50 µg /
€205.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY409843 | POLR2J2 overexpression lysate |
|
 |
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. |