POLR3GL antibody
Principal name
POLR3GL antibody
Alternative names for POLR3GL antibody
RNA polymerase III subunit C7-like, RPC32-like protein, RPC32HOM, DNA-directed RNA polymerase III subunit RPC7-like, DNA-directed RNA polymerase III subunit G-like
Ncbi ID
Available reactivities
Available hosts
Available applications
Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF)
Background of POLR3GL antibody
- Kit Component:
- - KN200991G1, POLR3GL gRNA vector 1 in pCas-Guide vector
- KN200991G2, POLR3GL gRNA vector 2 in pCas-Guide vector
- KN200991D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Haurie, V., et al. Proc. Natl. Acad. Sci. U.S.A. 107(9):4176-4181(2010)
Human MCF-7; WB Suggested Anti-POLR3GL Antibody. . Titration: 1.0 ug/ml. . Positive Control: MCF7 Whole Cell; POLR3GL antibody - C-terminal region (ARP60451_P050) in Human MCF-7 cells using Western Blot
Immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue reacted with POLR3GL Antibody (Center) Cat.-No AP53388PU-N, which was peroxidase conjugated to the secondary antibody and followed by DAB staining. This data demonstrates the use of POLR3GL Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Western blot analysis of POLR3GL Antibody (Center) Cat.-No AP53388PU-N in mouse kidney tissue lysates (35ug/lane). This demonstrates the POLR3GL antibody detected the POLR3GL protein (arrow).
Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-POLR3GL mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA506863)
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-POLR3GL mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA506863)
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-POLR3GL mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA506863)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-POLR3GL mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA506863)
Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-POLR3GL mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA506863)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-POLR3GL mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA506863)
Anti-POLR3GL mouse monoclonal antibody (TA506863) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY POLR3GL(RC200991).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY POLR3GL (RC200991, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-POLR3GL.
Anti-POLR3GL mouse monoclonal antibody (TA506864) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY POLR3GL(RC200991).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY POLR3GL (RC200991, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-POLR3GL.
Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-POLR3GL mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA506865)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-POLR3GL mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA506865)
Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-POLR3GL mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA506865)
Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-POLR3GL mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA506865)
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-POLR3GL mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA506865)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-POLR3GL mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA506865)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-POLR3GL mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA506865)
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-POLR3GL mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA506865)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-POLR3GL mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA506865)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-POLR3GL mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA506865)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-POLR3GL mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA506865)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-POLR3GL mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA506865)
Immunohistochemical staining of paraffin-embedded Human thyroid tissue within the normal limits using anti-POLR3GL mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA506865)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-POLR3GL mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA506865)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-POLR3GL mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA506865)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-POLR3GL mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA506865)
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-POLR3GL mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA506865)
Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-POLR3GL mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA506865)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-POLR3GL mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA506865)
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-POLR3GL mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA506865)
Anti-POLR3GL mouse monoclonal antibody (TA506865) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY POLR3GL(RC200991).
Immunofluorescent staining of HeLa cells using anti-POLR3GL mouse monoclonal antibody (TA506865).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY POLR3GL (RC200991, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-POLR3GL.
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-POLR3GL mouse monoclonal antibody. (TA506881)
Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-POLR3GL mouse monoclonal antibody. (TA506881)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-POLR3GL mouse monoclonal antibody. (TA506881)
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-POLR3GL mouse monoclonal antibody. (TA506881)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-POLR3GL mouse monoclonal antibody. (TA506881)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-POLR3GL mouse monoclonal antibody. (TA506881)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-POLR3GL mouse monoclonal antibody. (TA506881)
Anti-POLR3GL mouse monoclonal antibody (TA506881) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY POLR3GL(RC200991).
Immunofluorescent staining of HeLa cells using anti-POLR3GL mouse monoclonal antibody (TA506881).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY POLR3GL (RC200991, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-POLR3GL.
- Quick-Filters:
- Highly Validated (3)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA506863 | POLR3GL antibody |
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| POLR3GL mouse monoclonal antibody, clone OTI2F11 (formerly 2F11) | |||||||
 |
Mouse | IgG1 | OTI2F11 | Purified | Hu | ICC/IF, P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
 +7 additional images |
Highly validated antibody!
|
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| TA506865 | POLR3GL antibody |
||||||
| POLR3GL mouse monoclonal antibody, clone OTI5E8 (formerly 5E8) | |||||||
 |
Mouse | IgG1 | OTI5E8 | Purified | Hu | ICC/IF, P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +22 additional images |
Highly validated antibody!
|
||||||
| TA506881 | POLR3GL antibody |
||||||
| POLR3GL mouse monoclonal antibody, clone OTI5F9 (formerly 5F9) | |||||||
 |
Mouse | IgG1 | OTI5F9 | Purified | Hu | ICC/IF, P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +9 additional images |
Highly validated antibody!
|
||||||
| TA506864 | POLR3GL antibody |
||||||
| POLR3GL mouse monoclonal antibody, clone OTI5F10 (formerly 5F10) | |||||||
 |
Mouse | IgG1 | OTI5F10 | Purified | Hu | ICC/IF, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|
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| AP53388PU-N | POLR3GL (Center) antibody |
||||||
 |
Rabbit | Ig | Aff - Purified | Hu, Ms | P, WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies GmbH | |||||||
| +1 additional image |
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Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP300991 | POLR3GL |
||||
| Recombinant protein of human polymerase (RNA) III (DNA directed) polypeptide G (32kD)-like (POLR3GL) | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY410247 | POLR3GL overexpression lysate |
|
 |
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. |
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR202455L2V | Lenti ORF particles, Polr3gl (GFP-tagged) - Mouse polymerase (RNA) III (DNA directed) polypeptide G like (Polr3gl), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€930.00
|
|
| OriGene Technologies, Inc. | ||
| MR202455L1V | Lenti ORF particles, Polr3gl (Myc-DDK-tagged) - Mouse polymerase (RNA) III (DNA directed) polypeptide G like (Polr3gl), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€830.00
|
|
| OriGene Technologies, Inc. | ||