POMC antibody
Principal name
POMC antibody
Alternative names for POMC antibody
Pro-opiomelanocortin, Corticotropin-Lipotropin
SwissProt ID
P01189 (Human), P01190 (Bovin), P01191 (Sheep), P01192 (Pig), P01193 (Mouse), P01194 (Rat), P06297 (Rabit)
Ncbi ID
NP_000930.1, NP_000930, 9606, 10116, NM_001035256, NP_032921.1, NM_008895, NM_000939
Available reactivities
Available hosts
Available applications
Frozen Sections (C), Enzyme Immunoassay (E), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Dot blot (Dot), Immunoprecipitation (IP), Flow Cytometry (F)
Background of POMC antibody
The pro-opiomelanocortin (POMC) is the precursor of many pituitary hormones including Adenocorticotrphin (ACTH). The POMC was first identified in the anterior intermediate lobe of pituitary but is expressed in many other tissues including CNS, skin, and placenta. Following synthesis POMC undergoes extensive post-translational modifications and proteolysis producing not only ACTH but also a number of other biological active peptides. The proteolysis and post-translational modification are dependent upon tissue thus producing a different combination of peptides from the same precursor protein. These biological active peptides have diverse range of function ranging from pigmentation to adrenal and regulation of feeding behavior (1). ACTH is produced in the anterior intermediate lobe of the pituitary and in neurons in the brain. ACTH regulate the corticosteroid/glucocorticoid production in the adrenal cortex. Other hormones that are produced by POMC gene are beta endorphins, met enkaphalin (endogenous opiods), melanocyte stimulating hormone that increase the pigmentation of skin by increasing the production of melanin in melanocytes. Other hormone s are corticotrophin like intermediate lobe peptide (CLIP), Lipotrophins and endorphins. Defects in the POMC gene have been known to cause several defects including obesity. Ectopic immunodeficiency has been found in many tumors including prostate carcinoma, thymoma, pancreatic tumors. The testicular adrenal rest tumors are frequently associated with hyper adrenal hyperplasia, and ACTH-dependent tumors can be easily detected from leydig-cell tumors by ACTH staining (2). The serum lelves of ACTH are stimulated by adrenal-hypathamic-pituitary axis, ACTH levels are also positively correlated with stress levels.
Detection limit for recombinant GST tagged POMC is approximately 0.1ng/ml as a capture antibody.
Western Blot analysis of POMC expression in transfected 293T cell line (H00005443-T01) by POMC MaxPab polyclonal antibody.Lane 1: POMC transfected lysate(29.37 KDa).Lane 2: Non-transfected lysate.
POMC MaxPab polyclonal antibody. Western Blot analysis of POMC expression in K-562.
POMC polyclonal antibody ( Cat # PAB6777 ) ( 0.01 µg/ml ) staining of NIH/3T3 lysate ( 35 µg protein in RIPA buffer ) . Primary incubation was 1 hour. Detected by chemiluminescence.
POMC polyclonal antibody ( Cat # PAB6777 ) ( 2.5µg/ml ) staining of paraffin embedded Human Adrenal Gland. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Immunohistochemistry staining of human pituitary gland ( frozen sections ) with POMC monoclonal antibody, clone B31.15 ( Cat # MAB0905 ) .
Immunofluorescence of purified MaxPab antibody to POMC on HeLa cell. [antibody concentration 10 ug/ml]
Western Blot analysis of POMC expression in transfected 293T cell line (H00005443-T02) by POMC MaxPab polyclonal antibody.Lane 1: POMC transfected lysate(29.37 KDa).Lane 2: Non-transfected lysate.
AM11087PU ACTH antibody staining of Human Pituitary.
Human pituitary stained withAnti-ACTH antibody.
Immunohistochemical staining of POMC on formalin fixed, paraffin embedded human pituitary with POMC monoclonal antibody, clone SPM501.
Immunofluorescence of purified MaxPab antibody to POMC on HeLa cell. [antibody concentration 10 ug/ml]
Western Blot analysis of POMC expression in transfected 293T cell line (H00005443-T03) by POMC MaxPab polyclonal antibody.Lane 1: POMC transfected lysate(29.37 KDa).Lane 2: Non-transfected lysate.
Immunohistochemical staining of POMC on formalin fixed, paraffin embedded human pituitary with POMC polyclonal antibody.
Figure 1. Immunohistochemical analysis of Formalin-Fixed Paraffin-Embedded Human Adrenal tissue using this POMC antibody at 2.5 µg/ml.
Figure 2. POMC antibody staining of NIH 3T3 lysate at 0.01 µg/ml (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by Chemiluminescence.
Western Blot analysis of POMC expression in transfected 293T cell line (H00005443-T01) by POMC MaxPab polyclonal antibody.Lane 1: POMC transfected lysate(29.40 KDa).Lane 2: Non-transfected lysate.
Immunoprecipitation of POMC transfected lysate using anti-POMC MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with POMC MaxPab mouse polyclonal antibody (B01) (H00005443-B01).
Varying concentration of ACTH was blotted on Nitrocellulose and probed with 1/500 diluted AP09881PU-N antibody.
Human Testis (formalin-fixed, paraffin-embedded) stained with POMC antibody AP22703PU-N
Human Pancreas (formalin-fixed, paraffin-embedded) stained with POMC antibody AP22703PU-N
Human pituitary stained with Anti-ACTH antibody (AP22703PU-N)
Staining with ACTH (Adrenocorticotropic Hormone) Antibody (20070)
Recombinant human POMC, 27-267 aa, His-tagged
Formalin-fixed, Paraffin-Embedded Human pituitary gland stained with ACTH Antibody Cat.-No AM32828PU (Clone AH26) at 1/2000 using peroxidase-conjugate and DAB chromogen. Note cytoplasmic staining.
Formalin-paraffin human pituitary gland stained with ACTH Antibody Cat.-No AM50155PU (Clone SPM333).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY POMC (RC215351, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-POMC.
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-POMC mouse monoclonal antibody. (TA506608)
Immunofluorescent staining of HeLa cells using anti-POMC mouse monoclonal antibody (TA506608).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY POMC (RC215351, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-POMC.
Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-POMC mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA506610)
Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-POMC mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA506610)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-POMC mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA506610)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-POMC mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA506610)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY POMC (RC215351, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-POMC.
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-POMC mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA506611)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-POMC mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA506611)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY POMC (RC215351, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-POMC.
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-POMC mouse monoclonal antibody. (TA506615)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY POMC (RC215351, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-POMC.
TA303016 (0.01µg/ml) staining of NIH 3T3 lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
TA303016 (4ug/ml) staining of paraffin embedded Human Adrenal Gland. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.
Western blot analysis of Human fetal lung tissue using TA323524(POMC(Beta endorphin) Antibody) at dilution 1/150
Immunohistochemistry of paraffin-embedded Human tonsil tissue using TA323524(POMC.Beta endorphin. Antibody)(left image) at dilution 1/10, the image on the right is treated with the synthetic peptide.(Original magnification: x200)
Immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using TA323524(POMC.Beta endorphin. Antibody)(left image) at dilution 1/10, the image on the right is treated with the synthetic peptide.(Original magnification: x200)
Western blot analysis of A172 cell using TA323525(POMC(Beta endorphin) Antibody) at dilution 1/350
Predicted band size: 29 kDa . Positive control: Human liver cancer tissue lysate. Recommended dilution: 1/1000-5000. (Gel: 10%SDS-PAGE Lysate: 40 ug Primary antibody: 1/450 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute)
Predicted band size: 29 kDa . Positive control: Human liver cancer tissue lysate. Recommended dilution: 1/1000-5000. (Gel: 10%SDS-PAGE Lysate: 40 ug Primary antibody: 1/450 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute)
Predicted cell location: Nucleus. Positive control: Human brain tissue. Recommended dilution: 1/15-50 The image on the left is immunohistochemistry of paraffin-embedded Human brain tissue using TA323527 (POMC Antibody) at dilution 1/30, on the right is treated with the synthetic peptide. (Original magnification:×200)
Predicted band size: 29 kDa . Positive control: Human fetal brain tissue lysate. Recommended dilution: 1/500-2000
Predicted band size: 29 kDa . Positive control: Human fetal brain tissue lysate. Recommended dilution: 1/500-2000
Immunohistochemistry staining of Paraffin Pituitary tissue by ACTH antibody (dilution: 1:100 - 1:500; visualization of staining: Cytoplasmic)
Human Pituitary stained with anti-Adrenocorticotrophic Hormone (ACTH) antibody.
Human Pituitary stained with anti-Adrenocorticotrophic Hormone (ACTH) antibody.
- Quick-Filters:
- Highly Validated (1)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| AM32828PU-S | Adrenocorticotropic Hormone (ACTH) (1-24) antibody |
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 |
Mouse | IgG1 | AH26 | Purified | Br, Hu, Ms, Rt | C, F, ICC/IF, IP, P, WB |
0.1 mg /
€430.00
|
| OriGene Technologies GmbH | |||||||
OriGene recommends this product for Immunohistochemistry. |
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| AM32828PU-T | Adrenocorticotropic Hormone (ACTH) (1-24) antibody |
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|
Mouse | IgG1 | AH26 | Purified | Br, Hu, Ms, Rt | C, F, ICC/IF, IP, P, WB |
20 µg /
€240.00
|
| OriGene Technologies GmbH | |||||||
OriGene recommends this product for Immunohistochemistry. |
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| AM33348PU-S | Adrenocorticotropic Hormone (ACTH) (N-term) antibody |
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 |
Mouse | IgG1 | 57 | Purified | Hu, Rt | C, F, ICC/IF, IP, P, WB |
0.1 mg /
€430.00
|
| OriGene Technologies GmbH | |||||||
OriGene recommends this product for Immunohistochemistry. |
|||||||
| AM33348PU-T | Adrenocorticotropic Hormone (ACTH) (N-term) antibody |
||||||
|
Mouse | IgG1 | 57 | Purified | Hu, Rt | C, F, ICC/IF, IP, P, WB |
20 µg /
€240.00
|
| OriGene Technologies GmbH | |||||||
OriGene recommends this product for Immunohistochemistry. |
|||||||
| AM50155PU-S | Adrenocorticotropic Hormone (ACTH) (1-24) antibody |
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 |
Mouse | IgG1 | SPM333 | Purified | Hu, Ms, Rt | C, F, ICC/IF, IP, P, WB |
0.1 mg /
€430.00
|
| OriGene Technologies GmbH | |||||||
OriGene recommends this product for Immunohistochemistry. |
|||||||
| AM50155PU-T | Adrenocorticotropic Hormone (ACTH) (1-24) antibody |
||||||
|
Mouse | IgG1 | SPM333 | Purified | Hu, Ms, Rt | C, F, ICC/IF, IP, P, WB |
20 µg /
€240.00
|
| OriGene Technologies GmbH | |||||||
OriGene recommends this product for Immunohistochemistry. |
|||||||
| AM50199PU-S | Adrenocorticotropic Hormone (ACTH) (1-24) antibody |
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|
Mouse | IgG1 | 2F6 | Purified | Hu, Ms, Rt | C, F, ICC/IF, IP, P, WB |
0.1 mg /
€430.00
|
| OriGene Technologies GmbH | |||||||
OriGene recommends this product for Immunohistochemistry. |
|||||||
| AM50199PU-T | Adrenocorticotropic Hormone (ACTH) (1-24) antibody |
||||||
|
Mouse | IgG1 | 2F6 | Purified | Hu, Ms, Rt | C, F, ICC/IF, IP, P, WB |
20 µg /
€240.00
|
| OriGene Technologies GmbH | |||||||
OriGene recommends this product for Immunohistochemistry. |
|||||||
| AM50200PU-S | Adrenocorticotropic Hormone (ACTH) antibody |
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|
Mouse | IgG1 | AH26 + 57 | Purified | Hu, Ms, Rt | C, F, ICC/IF, IP, P, WB |
0.1 mg /
€430.00
|
| OriGene Technologies GmbH | |||||||
OriGene recommends this product for Immunohistochemistry. |
|||||||
| AM50200PU-T | Adrenocorticotropic Hormone (ACTH) antibody |
||||||
|
Mouse | IgG1 | AH26 + 57 | Purified | Hu, Ms, Rt | C, F, ICC/IF, IP, P, WB |
20 µg /
€240.00
|
| OriGene Technologies GmbH | |||||||
OriGene recommends this product for Immunohistochemistry. |
|||||||
| AM05431PU-N | Adrenocorticotropic Hormone (ACTH) (1-24) antibody |
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|
Mouse | IgG1 | 57 (BGN/1388/66) | Aff - Purified | Hu, Rt | E, P |
1 mg /
€720.00
|
| OriGene Technologies GmbH | |||||||
| AM05431PU-S | Adrenocorticotropic Hormone (ACTH) (1-24) antibody |
||||||
|
Mouse | IgG1 | 57 (BGN/1388/66) | Aff - Purified | Hu, Rt | E, P |
0.2 mg /
€460.00
|
| OriGene Technologies GmbH | |||||||
| AM32066PU-N | Adrenocorticotropic Hormone (ACTH) antibody |
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|
Mouse | IgG1 | 57 | Purified | Hu | E, P |
1 mg /
€630.00
|
| OriGene Technologies GmbH | |||||||
| AP05348PU-N | Adrenocorticotropic Hormone (ACTH) antibody |
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|
Rabbit | IgG | Purified | Br, Hu | C, E, P |
0.5 ml /
€410.00
|
|
| OriGene Technologies GmbH | |||||||
| AP22703PU-N | Adrenocorticotropic Hormone (ACTH) (N-term) antibody |
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 |
Rabbit | Purified | Hu | P |
0.25 ml /
€425.00
|
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| OriGene Technologies GmbH | |||||||
 +2 additional images |
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| RA21005-50 | Adrenocorticotropic Hormone (ACTH) antibody |
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 |
Rabbit | IgG | Aff - Purified | Hu, Rt | C, ICC/IF, WB |
50 µg /
€240.00
|
|
| Neuromics Antibodies | |||||||
| RA21003-50 | Lipotropin beta antibody |
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|
Rabbit | Serum | Hu, Ms, Rt | C, ICC/IF |
50 µl /
€240.00
|
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| Neuromics Antibodies | |||||||
| RA20074-100 | MSH alpha antibody |
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 |
Rabbit | IgG | Serum | GP, Ms, Rt | C, ICC/IF |
0.1 ml /
€370.00
|
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| Neuromics Antibodies | |||||||
| TA506610 | POMC antibody |
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| POMC mouse monoclonal antibody, clone OTI2B2 (formerly 2B2) | |||||||
 |
Mouse | IgG2a | OTI2B2 | Purified | Hu | P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +4 additional images |
Highly validated antibody!
|
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| TA303016 | POMC (C-term) antibody |
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| Goat Polyclonal Antibody against POMC | |||||||
 |
Goat | Aff - Purified | Hu, Ms, Rt | P, WB |
0.1 mg /
€360.00
|
||
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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