PON2 antibody
Principal name
PON2 antibody
Alternative names for PON2 antibody
PON-2, PON 2, Aromatic esterase 2, A-esterase 2, Serum paraoxonase/arylesterase 2, Serum aryldialkylphosphatase 2
SwissProt ID
P54832 (Canfa), Q15165 (Human), Q58DS7 (Bovin), Q62086 (Mouse), Q6AXM8 (Rat), Q90952 (Chick)
Ncbi ID
9606, NP_000296.2, NP_000296, NP_899131.1, BC021887, NM_183308, NM_001013082, NM_000305, NM_001018161
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunoprecipitation (IP), Flow Cytometry (F), Frozen Sections (C)
Background of PON2 antibody
- Kit Component:
- - KN313632G1, Pon2 gRNA vector 1 in pCas-Guide vector
- KN313632G2, Pon2 gRNA vector 2 in pCas-Guide vector
- KN313632D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Altenhofer, S., et al. J. Biol. Chem. 285(32):24398-24403(2010)
Ticozzi, N., et al. Ann. Neurol. 68(1):102-107(2010)
Wang, Y., et al. Diabet. Med. 27(4):376-383(2010)
Cross, D.S., et al. BMC Genet. 11, 51 (2010)
Immunuhistochemistry: AP07128PU-N PON2 antibody staining of Formalin-Fixed Paraffin-Embedded Human Liver at 20 µg/ml followed by biotinylated Goat anti-Rabbit IgG secondaryantibody, Alkaline Phosphatase-Streptavidin and chromogen.
Immunoblot analysis of cell lysates.Lane 1 : HeLa cell lysate. Lnae 2 : HepG2 cell lysate.Lane 3 : 293T cell lysate.
Immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue reacted with PON2 Antibody (N-term) Cat.-No AP53391PU-N, which was peroxidase conjugated to the secondary antibody and followed by DAB staining. This data demonstrates the use of PON2 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Western blot analysis of PON2 Antibody (N-term) Cat.-No AP53391PU-N in HepG2 cell line lysates (35ug/lane). This demonstrates the PON2 antibody detected the PON2 protein (arrow).
Flow cytometric analysis of K562 cells using PON2 Antibody (N-term) Cat.-No AP53391PU-N (right histogram) compared to a negative control cell (left histogram). FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.
Sample (30 ug of whole cell lysate). A: A431 . 10% SDS PAGE. PON2 antibody. TA308448 diluted at 1:1000.
Sample (50 ug of whole cell lysate). A: mouse liver. 10% SDS PAGE. TA308448 diluted at 1:1000.
Immunohistochemical analysis of paraffin-embedded N87 xenograft, using PON2(TA308448) antibody at 1:100 dilution.
(0.2ug/ml) staining of HepG2 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Predicted band size: 39 kDa . Positive control: HepG2 and 293T cell lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lane1: HepG2 cell lysate Lane2: 293T cell lysate Lysate: 40 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute)
TA348946 (2 ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA308448 | PON2 antibody |
||||||
| Rabbit Polyclonal antibody to PON2 (paraoxonase 2) | |||||||
 |
Rabbit | IgG | Aff - Purified | Hu, Ms | P, WB |
0.1 ml /
€415.00
|
|
| OriGene Technologies, Inc. | |||||||
 +2 additional images |
|||||||
| TA311123 | PON2 antibody |
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| Goat Anti-PON2 Antibody | |||||||
 |
Goat | Aff - Purified | Can, Hu, Por, Rt | E, WB |
0.1 mg /
€360.00
|
||
| OriGene Technologies, Inc. | |||||||
| TA321106 | PON2 antibody |
||||||
| Anti-PON2 Rabbit Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA348946 | PON2 antibody |
||||||
| Goat Polyclonal Anti-PON2 Antibody | |||||||
 |
Goat | Purified | Bov, Can, Hu, Ms, Por, Rt | P |
0.1 mg /
€360.00
|
||
| OriGene Technologies, Inc. | |||||||
| AP53391PU-N | PON2 (N-term) antibody |
||||||
 |
Rabbit | Ig | Aff - Purified | Hu | F, P, WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies GmbH | |||||||
| +2 additional images |
|||||||
| AP55270SU-N | PON2 antibody |
||||||
 |
Rabbit | Serum | Hu | C, E, WB |
0.2 ml /
€430.00
|
||
| OriGene Technologies GmbH | |||||||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP322624 | PON2 (transcript variant 1) |
||||
| Recombinant protein of human paraoxonase 2 (PON2), transcript variant 1 | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR202043L2V | Lenti ORF particles, Pon2 (GFP-tagged) - Mouse paraoxonase 2 (cDNA clone MGC:11614 IMAGE:3154583), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€840.00
|
|
| OriGene Technologies, Inc. | ||
| MR205388L2V | Lenti ORF particles, Pon2 (GFP-tagged) - Mouse paraoxonase 2 (Pon2), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€930.00
|
|
| OriGene Technologies, Inc. | ||
| MR202043L1V | Lenti ORF particles, Pon2 (Myc-DDK-tagged) - Mouse paraoxonase 2 (cDNA clone MGC:11614 IMAGE:3154583), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€740.00
|
|
| OriGene Technologies, Inc. | ||
| MR205388L1V | Lenti ORF particles, Pon2 (Myc-DDK-tagged) - Mouse paraoxonase 2 (Pon2), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€830.00
|
|
| OriGene Technologies, Inc. | ||
Rat Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RR200686L2V | Lenti ORF particles, Pon2 (GFP-tagged ORF) - Rat paraoxonase 2 (Pon2), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€930.00
|
|
| OriGene Technologies, Inc. | ||
| RR200686L1V | Lenti ORF particles, Pon2 (Myc-DDK-tagged ORF) - Rat paraoxonase 2 (Pon2), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€830.00
|
|
| OriGene Technologies, Inc. | ||