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POU4F3 antibody

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Principal name

POU4F3 antibody

Alternative names for POU4F3 antibody

BRN3C, Brain-3C, Brn-3C, POU domain class 4 transcription factor 3, Brain-specific homeobox/POU domain protein 3C

SwissProt ID

Q15319 (Human), Q63955 (Mouse), Q90435 (Danre)

Gene ID

5459 (POU4F3), 18998 (Pou4f3)

Ncbi ID

9606, NP_002691, NM_138945, NP_002691.1, NM_002700, 10090

Available reactivities

Hu (Human), Bov (Bovine), Can (Canine), Chk (Chicken), Ms (Mouse), Por (Porcine), Rt (Rat), Ze (Zebrafish), African clawed frog, Eq (Equine), GP (Guinea Pig), Rb (Rabbit)

Available hosts

Mouse, Rabbit, Goat

Available applications

Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Immunoprecipitation (IP)

Background of POU4F3 antibody

Kit Component:
- KN313657G1, Pou4f3 gRNA vector 1 in pCas-Guide vector
- KN313657G2, Pou4f3 gRNA vector 2 in pCas-Guide vector
- KN313657D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector
CRISPR Manual:
CRISPR/CAS9 Genome Editing Manual

General readings

Weiss,S., et al., (2003) Mol. Cell. Biol. 23 (22), 7957-7964

H00005459-M01 - POU4F3
Immunofluorescence of monoclonal antibody to POU4F3 on HeLa cell. [antibody concentration 10 ug/ml]
H00005459-M01 - POU4F3
Western blot analysis of POU4F3 over-expressed 293 cell line, cotransfected with POU4F3 Validated Chimera RNAi ( Cat # H00005459-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with POU4F3 monoclonal antibody (M01), clone 5B8 (Cat # H00005459-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
H00005459-M01 - POU4F3
POU4F3 monoclonal antibody (M01), clone 5B8 Western Blot analysis of POU4F3 expression in HeLa NE ( Cat # L013V3 ).
H00005459-M01 - POU4F3
Western Blot analysis of POU4F3 expression in transfected 293T cell line by POU4F3 monoclonal antibody (M01), clone 5B8.Lane 1: POU4F3 transfected lysate(37.1 KDa).Lane 2: Non-transfected lysate.
H00005459-M01 - POU4F3
Detection limit for recombinant GST tagged POU4F3 is approximately 0.1ng/ml as a capture antibody.
ARP33065_T100 - POU4F3
Human kidney; POU4F3 antibody - middle region (ARP33065_T100) in Human kidney cells using Immunohistochemistry
ARP33065_T100 - POU4F3
Human HepG2; WB Suggested Anti-POU4F3 Antibody Titration: 1.25ug/ml. Positive Control: HepG2 cell lysate; POU4F3 antibody - middle region (ARP33065_T100) in Human HepG2 cells using Western Blot
H00005459-B01P - POU4F3
Western Blot analysis of POU4F3 expression in transfected 293T cell line (H00005459-T01) by POU4F3 MaxPab polyclonal antibody.Lane 1: POU4F3 transfected lysate(37.18 KDa).Lane 2: Non-transfected lysate.
H00005459-D01 - POU4F3
Immunoprecipitation of POU4F3 transfected lysate using anti-POU4F3 MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with POU4F3 MaxPab mouse polyclonal antibody (B01) (H00005459-B01).
ARP33064_P050 - POU4F3
Mouse Heart; WB Suggested Anti-Pou4f3 Antibody. . Titration: 1.0 ug/ml. . Positive Control: Mouse Heart; Pou4f3 antibody - N-terminal region (ARP33064_P050) in Mouse Heart cells using Western Blot
CPA1914-100ul - POU4F3
Western blot analysis of BRN3C expression in HeLa (A); Jurkat (B); Raw264.7 (C); H9C2 (D) whole cell lysates.
TA808720 - POU4F3
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY POU4F3 (RC211206, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-POU4F3.(1:2000)
TA808728 - POU4F3
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY POU4F3 (RC211206, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-POU4F3.(1:2000)
TA808731 - POU4F3
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY POU4F3 (RC211206, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-POU4F3.(1:2000)
TA808755 - POU4F3
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY POU4F3 (RC211206, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-POU4F3.(1:2000)
TA808756 - POU4F3
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY POU4F3 (RC211206, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-POU4F3.(1:2000)
TA808757 - POU4F3
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY POU4F3 (RC211206, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-POU4F3.(1:2000)
TA311245 - POU4F3
(0.2ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
TA314363 - POU4F3
Western blot analysis of extracts from HuvEc cells, using POU4F3 antibody.The lane on the right is treated with the synthesized peptide.
TA332118 - POU4F3
WB Suggested Anti-POU4F3 Antibody Titration: 1.25ug/ml; Positive Control: HepG2 cell lysate
TA332118 - POU4F3
Human kidney

9 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

POU4F3 antibody
  Goat Anti-POU4F3 Antibody  
(0.2ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Can, Hu, Ms, Rt E, WB
0.1 mg / €360.00
  OriGene Technologies, Inc.

POU4F3 antibody
  Rabbit polyclonal anti-POU4F3 antibody  
Western blot analysis of extracts from HuvEc cells, using POU4F3 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, WB
0.1 ml / €335.00
  OriGene Technologies, Inc.

POU4F3 antibody
  POU4F3 mouse monoclonal antibody, clone OTI4C5 (formerly 4C5)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY POU4F3 (RC211206, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-POU4F3.(1:2000) Mouse IgG1 OTI4C5 Purified Hu WB
0.1 ml / €360.00
  OriGene Technologies, Inc.
 

TrueMAB

POU4F3 antibody
  POU4F3 mouse monoclonal antibody, clone OTI4G7 (formerly 4G7)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY POU4F3 (RC211206, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-POU4F3.(1:2000) Mouse IgG1 OTI4G7 Purified Hu WB
0.1 ml / €360.00
  OriGene Technologies, Inc.
 

TrueMAB

POU4F3 antibody
  POU4F3 mouse monoclonal antibody, clone OTI3A12 (formerly 3A12)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY POU4F3 (RC211206, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-POU4F3.(1:2000) Mouse IgG1 OTI3A12 Purified Hu WB
0.1 ml / €360.00
  OriGene Technologies, Inc.
 

TrueMAB

POU4F3 antibody
  POU4F3 mouse monoclonal antibody, clone OTI6H9 (formerly 6H9)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY POU4F3 (RC211206, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-POU4F3.(1:2000) Mouse IgG1 OTI6H9 Purified Hu WB
0.1 ml / €360.00
  OriGene Technologies, Inc.
 

TrueMAB

POU4F3 antibody
  POU4F3 mouse monoclonal antibody, clone OTI6F4 (formerly 6F4)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY POU4F3 (RC211206, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-POU4F3.(1:2000) Mouse IgG1 OTI6F4 Purified Hu WB
0.1 ml / €360.00
  OriGene Technologies, Inc.
 

TrueMAB

POU4F3 antibody
  POU4F3 mouse monoclonal antibody, clone OTI2F11 (formerly 2F11)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY POU4F3 (RC211206, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-POU4F3.(1:2000) Mouse IgG1 OTI2F11 Purified Hu WB
0.1 ml / €360.00
  OriGene Technologies, Inc.
 

TrueMAB

Lysates

Catalog No.    

POU4F3 overexpression lysate
POU4F3 overexpression lysate  
0.1 mg / €295.00
  OriGene Technologies, Inc.

9 Item(s)

per page

Available Filters

Product Type
  1. Lysate (1)
  2. Primary Antibody (8)
Clonality
  1. Monoclonal (6)
  2. Polyclonal (2)
Target
  1. POU4F3 (8)
Reactivity
  1. Human (Hu) (8)
  2. Mouse (Ms) (2)
  3. Canine (Can) (1)
  4. Rat (Rt) (1)
Applications
  1. Western blot / Immunoblot (WB) (8)
  2. Enzyme Immunoassay (E) (2)
Presentation
  1. Purified (7)
  2. Aff - Purified (1)
Host
  1. Mouse (6)
  2. Goat (1)
  3. Rabbit (1)
Images
  1. 1 image (8)
Images: Applications
  1. Western blot / Immunoblot (WB) (8)
Manufacturer
  1. OriGene Technologies (9)
Service
  • OriGene Technologies GmbH
  • Schillerstraße 5
  • D-32052 Herford
  • Germany
  • phone: +49-5221-34606-0
  • free call: 0800-227 47 46 (Germany only)
  • fax: +49-5221-34606-11
  • email: info-de@origene.com
  • orders: ordering-de@origene.com
 
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