PPME1 antibody
Principal name
PPME1 antibody
Alternative names for PPME1 antibody
PME1, PME-1, Protein phosphatase methylesterase 1
SwissProt ID
P38796 (Yeast), Q58DN4 (Bovin), Q8BVQ5 (Mouse), Q9BIB3 (Caeel), Q9P7D2 (Schpo), Q9Y570 (Human)
Ncbi ID
Available reactivities
Available hosts
Available applications
ELISA (detection) (E(detection)), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Flow Cytometry (F)
Background of PPME1 antibody
Carboxymethylation of proteins is a highly conserved means of regulation in eukaryotic cells. The serine hydrolase protein phosphatase methylesterase-1 (PME-1) regulates the methylesterification state of protein phosphatase 2A (PP2A) and has been implicated in cancer and neurodegeneration disease. PME-1 is conserved from yeast to human and contains a motif found in lipases having a catalytic triad-activated serine as their active site nucleophile. Maturation of catalytic subunit of PP2A is under the surveillance of the PP2A methylesterase, PME1, which upon malfunction of PP2A biogenesis, prevents premature generation of the active C subunit and holoenzyme assembly by counteracting the untimely methylation of the C subunit. PME-1 has been recently identified as a protector of sustained ERK pathway activity in malignant gliomas. Mechanistically, PME-1 is shown to support ERK pathway signaling upstream of Raf, but downstream of growth factor receptors and protein kinase C. In malignant gliomas, PME-1 expression levels correlate with both ERK activity and cell proliferation .
General readings
Gagnon S.N. et al. (2002) Biochem. J. 368:263-271.
Western Blot analysis of PPME1 expression in transfected 293T cell line (H00051400-T01) by PPME1 MaxPab polyclonal antibody.Lane 1: PPME1 transfected lysate(42.46 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of PPME1 expression in transfected 293T cell line (H00051400-T01) by PPME1 MaxPab polyclonal antibody.Lane 1: PPME1 transfected lysate(42.46 KDa).Lane 2: Non-transfected lysate.
Human brain; Immunohistochemistry with Brain, cortex tissue at an antibody concentration of 5ug/ml using anti-PPME1 antibody (ARP56845_P050); PPME1 antibody - N-terminal region (ARP56845_P050) in Human brain cells using Immunohistochemistry
Human Pineal Tissue; Rabbit Anti-PPME1 Antibody . Catalog Number: ARP56845_P050 . Formalin Fixed Paraffin Embedded Tissue: Human Pineal Tissue . Observed Staining: Cytoplasmic in cell bodies of pinealocytes . Primary Antibody Concentration: 1:100 . Secondary Antibody: Donkey anti-Rabbit-Cy3 . Secondary Antibody Concentration: 1:200 . Magnification: 20X . Exposure Time: 0.5 - 2.0 sec .; Rabbit Anti-PPME1 Antibody (ARP56845_P050) ) in Human Pineal Tissue using Immunohistochemistry
Human 721_B; WB Suggested Anti-PPME1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: 721_B cell lysate. PPME1 is supported by BioGPS gene expression data to be expressed in 721_B.; PPME1 antibody - N-terminal region (ARP56845_P050) in Human 721_B cells using Western Blot
Human Fetal Brain; Host:Rabbit. Target Name:PPME1. Sample Tissue:Human Fetal Brain. Antibody Dilution: 1.0ug/ml.; PPME1 antibody - N-terminal region (ARP56844_P050) in Human Fetal Brain cells using Western Blot
Human NCI-H226; WB Suggested Anti-PPME1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: NCI-H226 cell lysate. PPME1 is strongly supported by BioGPS gene expression data to be expressed in NCI-H226.; PPME1 antibody - N-terminal region (ARP56844_P050) in Human NCI-H226 cells using Western Blot
Rcombinant human PPME1, 1-386aa, His-tagged
Immunohistochemical staining of paraffin-embedded Ovary tissue within the normal limits using anti-PPME1mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500768, Dilution 1:50)
Anti-PPME1 mouse monoclonal antibody (TA500768) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PPME1(RC200009).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PPME1 (RC200009, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PPME1.
Anti-PPME1 mouse monoclonal antibody (TA500769) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PPME1(RC200009).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PPME1 (RC200009, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PPME1.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PPME1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).(1:200)
HEK293T cells transfected with either pCMV6-ENTRY PPME1(RC200009)(Red) or empty vector control plasmid(Blue) were immunostained with anti-PPME1 mouse monoclonal(TA500769, Dilution 1:1,000), and then analyzed by flow cytometry.
Immunohistochemical staining of paraffin-embedded Kidney tissue within the normal limits using anti-PPME1mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500821, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded liver tissue within the normal limits using anti-PPME1mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500821, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded Carcinoma of liver tissue using anti-PPME1mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500821, Dilution 1:50)
Anti-PPME1 mouse monoclonal antibody (TA500821) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PPME1(RC200009).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PPME1 (RC200009, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PPME1.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PPME1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).(1:200)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-PPME1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500822, Dilution 1:50)
Anti-PPME1 mouse monoclonal antibody (TA500822) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PPME1(RC200009).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PPME1 (RC200009, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PPME1.
HEK293T cells transfected with either pCMV6-ENTRY PPME1(RC200009)(Red) or empty vector control plasmid(Blue) were immunostained with anti-PPME1 mouse monoclonal(TA500822), and then analyzed by flow cytometry.
Immunohistochemical staining of paraffin-embedded Kidney tissue within the normal limits using anti-PPME1mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500823, Dilution 1:50)
Anti-PPME1 mouse monoclonal antibody (TA500823) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PPME1(RC200009).
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PPME1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).(1:200)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PPME1 (RC200009, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PPME1.
Flow cytometric analysis of Hela cells, using anti-PPME1 antibody(TA500823),(Red) compared to a nonspecific negative control antibody(TA50011)(Blue).
Flow cytometric analysis of Jurkat cells, using anti-PPME1 antibody(TA500823),(Red) compared to a nonspecific negative control antibody(TA50011)(Blue).
Immunohistochemical staining of paraffin-embedded Carcinoma of liver tissue using anti-PPME1mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500840, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded Kidney tissue within the normal limits using anti-PPME1mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500840, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded liver tissue within the normal limits using anti-PPME1mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500840, Dilution 1:50)
Anti-PPME1 mouse monoclonal antibody (TA500840) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PPME1(RC200009).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PPME1 (RC200009, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PPME1.
WB Suggested Anti-PPME1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: 721_B cell lysatePPME1 is supported by BioGPS gene expression data to be expressed in 721_B
Immunohistochemistry with Brain, cortex tissue at an antibody concentration of 5 ug/ml using anti-PPME1 antibody
Rabbit Anti-PPME1 Antibody; Formalin Fixed Paraffin Embedded Tissue: Human Pineal Tissue; Observed Staining: Cytoplasmic in cell bodies of pinealocytes; Primary Antibody Concentration: 1:100; Secondary Antibody: Donkey anti-Rabbit-Cy3; Secondary Antibody
Host:Rabbit; Target Name:PPME1; Sample Tissue:Human Fetal Brain; Antibody Dilution: 1.0 ug/ml
WB Suggested Anti-PPME1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:1562500; Positive Control: NCI-H226 cell lysatePPME1 is strongly supported by BioGPS gene expression data to be expressed in NCI-H226
CRISPR
| Catalog No. | Species | Donor Vector | |
|---|---|---|---|
| KN200009 | PPME1 - human gene knockout kit via CRISPR |
||
 |
Human | GFP-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN200009BN | PPME1 - human gene knockout kit via CRISPR |
||
|
Human | mBFP-Neo |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN200009LP | PPME1 - human gene knockout kit via CRISPR |
||
|
Human | Luciferase-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN200009RB | PPME1 - human gene knockout kit via CRISPR |
||
|
Human | RFP-BSD |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
