PPP1CB antibody
Principal name
PPP1CB antibody
Alternative names for PPP1CB antibody
PP-1B, PP1beta, Protein phosphatase 1-beta, PPP1CD, Protein phosphatase 1-delta, Serine/threonine-protein phosphatase PP1-beta catalytic subunit
SwissProt ID
P48462 (Drome), P61292 (Pig), P62140 (Human), P62141 (Mouse), P62142 (Rat), P62143 (Rabit), P62207 (Chick), Q3SWW9 (Bovin), Q6GQL2 (Xenla), Q8MJ47 (Canfa)
Ncbi ID
9606, NP_002700.1, NP_996759, NP_766295.2, NM_172707, NM_206876, NM_002709
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Immunoprecipitation (IP), Paraffin Sections (P)
Background of PPP1CB antibody
- Kit Component:
- - KN201142G1, PPP1CB gRNA vector 1 in pCas-Guide vector
- KN201142G2, PPP1CB gRNA vector 2 in pCas-Guide vector
- KN201142D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
Western Blot analysis of PPP1CB expression in transfected 293T cell line (H00005500-T01) by PPP1CB MaxPab polyclonal antibody.Lane 1: PPP1CB transfected lysate(35.97 KDa).Lane 2: Non-transfected lysate.
PP 1 beta antibody western blot of total rat brain homogenate. (+) indicates the presence of specific blocking peptide or (-) the presence of control peptide.
The PPP1CB polyclonal antibody ( Cat # PAB4113 ) is used in Western blot to detect PPP1CB in mouse brain tissue lysate.
Western Blot analysis of PPP1CB expression in transfected 293T cell line (H00005500-T02) by PPP1CB MaxPab polyclonal antibody.Lane 1: PPP1CB transfected lysate(35.97 KDa).Lane 2: Non-transfected lysate.
PPP1CB polyclonal antibody ( Cat # PAB10412 ) in western blot of total rat brain homogenate. (+) indicates the presence of specific blocking peptide or (-) the presence of control peptide.
Immunoblot analysis of cell lysates.Lane 1 : Jurkat cell lysate. Lnae 2 : MOLT 4 cell lysate.Lane 3 : HT-29 cell lysate.Lane 4 : A-431 cell lysate.Lane 5 : SK-N-SH cell lysate.Lane 6 : PC-12 cell lysate.Lane 7 : NIH/3T3 cell lysate.
Predicted band size: 37 kDa . Positive control: Hela cell lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lysate: 40 ug per lane Primary antibody: 1/450dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 20 seconds)
Predicted cell location: Cytoplasm, Nucleus. Positive control: Human tonsil tissue. Recommended dilution: 1/25-100 The image on the left is immunohistochemistry of paraffin-embedded human tonsil tissue using PPP1CB antibody at dilution 1/30, on the right is treated with the fusion protein. (Original magnification: x200)
Predicted cell location: Cytoplasm. Positive control: Human ovarian and lung cancer tissue. Recommended dilution: 1/50-200 The image on the left is immunohistochemistry of paraffin-embedded human ovarian cancer tissue using PPP1CB antibody at dilution 1/40, on the right is treated with the fusion protein. (Original magnification:x 200)
Predicted cell location: Cytoplasm. Positive control: Human ovarian and lung cancer tissue. Recommended dilution: 1/50-200 The image on the left is immunohistochemistry of paraffin-embedded human lung cancer tissue using PPP1CB antibody at dilution 1/40, on the right is treated with the fusion protein. (Original magnification:x200)
Western blot analysis of PPP1CB (arrow) using rabbit polyclonal PPP1CB Antibody (K301) (Cat. #TA325036). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PPP1CB gene.
IF image of U251 cell stained with PPP1CB Antibody (C-term)(Cat#TA325036).U251 cells were incubated with PPP1CB primary antibody (1:25, 1 h at 37?). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7 units/ml).PPP1CB immunoreactivity is localized to Cytoplasm and Nucleus significantly.
Western blot analysis of Jurkat cell and brain cell lysate using PPP1CB antibody.
Immunohistochemistry of paraffin-embedded human lung cancer using PPP1CB antibody at dilution of 1:200 (200x lens).
Immunofluorescence analysis of U2OS cell using PPP1CB antibody. Blue: DAPI for nuclear staining.
WB Suggested Anti-PPP1CB Antibody; Titration: 1.0 ug/ml; Positive Control: Fetal Liver
WB Suggested Anti-PPP1CB Antibody; Titration: 1.0 ug/ml; Positive Control: Fetal Kidney
- Quick-Filters:
- Cited (1)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA321512 | PPP1CB antibody |
||||||
| Anti-PPP1CB Rabbit Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, P, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
 +1 additional image |
|||||||
| TA321513 | PPP1CB antibody |
||||||
| Anti-PPP1CB Rabbit Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, P |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|||||||
| TA325036 | PPP1CB (C-term) antibody |
||||||
| Rabbit polyclonal Protein Phosphatase 1 beta (PPP1CB) Antibody (C-term) | |||||||
 |
Rabbit | Ig | Purified | Hu, Ms | WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|||||||
| TA326920 | PPP1CB antibody |
||||||
| Rabbit anti-PPP1CB Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt |
0.1 mg /
€335.00
|
||
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
|||||||
| AP05034PU-N | PPP1CB antibody |
||||||
 |
Rabbit | IgG | Purified | Bov, Hu, Ms, Rt | IP, WB |
0.1 mg /
€400.00
|
|
| OriGene Technologies GmbH | |||||||
| AP05035PU-N | PPP1CB antibody |
||||||
 |
Sheep | IgG | Purified | Bov, Hu, Ms, Rt | IP, WB |
0.1 mg /
€400.00
|
|
| OriGene Technologies GmbH | |||||||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP301142 | PPP1CB (transcript variant 3) |
||||
| Recombinant protein of human protein phosphatase 1, catalytic subunit, beta isoform (PPP1CB), transcript variant 3 | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Published recently! |
|||||
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR204795L1V | Lenti ORF particles, Ppp1cb (Myc-DDK-tagged) - Mouse protein phosphatase 1, catalytic subunit, beta isoform (Ppp1cb), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€830.00
|
|
| OriGene Technologies, Inc. | ||
