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PPP1R13L antibody

Principal name

PPP1R13L antibody

Alternative names for PPP1R13L antibody

RelA-associated inhibitor, IASPP, NKIP1, PPP1R13BL, RAI, Inhibitor of ASPP protein, NFkB-interacting protein 1, PPP1R13B-like protein

SwissProt ID

Q5I1X5 (Mouse), Q8WUF5 (Human), Q9XVN3 (Caeel)

Gene ID

10848 (PPP1R13L), 333654 (Ppp1r13l)

Ncbi ID

9606, NP_001135974.1, NP_006654, NP_001010836.1, NM_001010836, NM_006663, NM_001142502

Available reactivities

Hu (Human), Bov (Bovine), Can (Canine), Ms (Mouse), Ze (Zebrafish), African clawed frog, Rt (Rat), Eq (Equine), GP (Guinea Pig), Rb (Rabbit), Por (Porcine)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), ELISA (detection) (E(detection))

Background of PPP1R13L antibody

ASPP proteins (ASPP1, ASPP2 and iASPP) represent a new family of p53 binding proteins. ASPP1 and ASPP2 bind and enhance p53-mediated apoptosis. In contrast, the third member, iASPP, functionally inactivates p53. iASPP (also called protein phosphatase 1 regulatory (inhibitor) subunit 13 like protein, Inhibitor of ASPP protein, Protein iASPP, PPP1R13B-like protein and NFkappaB-interacting protein 1) plays a central role in regulation of apoptosis and transcription via its interaction with NF-kappa-B and p53/TP53 proteins. iASPP blocks transcription of HIV-1 virus by inhibiting the action of both NF-kappa-B and SP1. This protein also inhibits p53/TP53 function, possibly by preventing the association between p53/TP53 and ASPP1 or ASPP2, and therefore suppressing the subsequent activation of apoptosis. iASPP is predominantly a cytoplasmic protein (isoform 1) but can also be found in the nucleus (isoform 2). iASPP is highly expressed in heart, placenta and prostate and is weakly expressed in brain, liver, skeletal muscle, testis and peripheral blood leukocyte. The N-terminal region of isoform 1 is required for cytoplasmic localization. Defects in iASPP may be a cause of certain breast cancers and the protein is overexpressed in many patients suffering from breast carcinomas and expressing a wild-type p53/TP53 protein.

General readings

Tomoda,K., (2008) Cancer Sci. 99 (3), 615-622

Figure 1. Formalin-Fixed Paraffin-Embedded (FFPE) on Liver.

AP07622PU-N

Immunofluorescence of purified MaxPab antibody to PPP1R13L on HeLa cell. [antibody concentration 10 ug/ml]

H00010848-B01

Western Blot analysis of PPP1R13L expression in transfected 293T cell line (H00010848-T01) by PPP1R13L MaxPab polyclonal antibody.Lane1:PPP1R13L transfected lysate(91.08 KDa).Lane2:Non-transfected lysate.

H00010848-B01

Human A549; . Sample Type :. Human lung adenocarcinoma cell line A549. Primary Antibody Dilution :. 1:100. Secondary Antibody :. Goat anti-rabbit AlexaFluor 488. Secondary Antibody Dilution :. 1:400. Color/Signal Descriptions :. PPP1R13L: Green DAPI:Blue. Gene Name :. PPP1R13L . Submitted by :. Dr. Crissy Dudgeon, Ph.D., CINJ; PPP1R13L antibody - middle region (ARP32826_P050) in Human A549 cells using Immunohistochemistry

ARP32826_P050

Transfected 293T; WB Suggested Anti-PPP1R13L Antibody Titration: 0.2-1 ug/ml. Positive Control: Transfected 293T; PPP1R13L antibody - middle region (ARP32826_P050) in Transfected 293T cells using Western Blot

ARP32826_P050

Human; . Lanes: 1. 30 ug MiaPaca-2 cell lysate. 2. 30 ug Panc-1 cell lysate. Primary Antibody Dilution: 1:1000. Secondary Antibody: Goat anti-Rabbit HRP. Secondary Antibody Dilution: 1:4000. Gene Name: PPP1R13L. Submitted by: Dr. Crissy Dudgeon, Ph.D., CINJ.; PPP1R13L antibody - middle region (ARP32826_P050) in Human cells using Western Blot

ARP32826_P050

Immunofluorescence of purified MaxPab antibody to PPP1R13L on HeLa cell. [antibody concentration 10 ug/ml]

H00010848-B01P

H00010848-B01P

Western Blot analysis of PPP1R13L expression in transfected 293T cell line (H00010848-T02) by PPP1R13L MaxPab polyclonal antibody.Lane 1: PPP1R13L transfected lysate(89.10 KDa).Lane 2: Non-transfected lysate.

H00010848-D01P

Immunohistochemistry of PPP1R13L polyclonal antibody ( Cat # PAB10243 ) shows strong cytoplasmic and membranous staining of myocytes in human heart tissue.Tissue was formalin-fixed and paraffin embedded.Brown color indicates presence of protein, blue color shows cell nuclei.Personal Communication, Kenneth Wester, www.proteinatlas.org, Uppsala, Sweden.

PAB10243

Western blot using PPP1R13L polyclonal antibody ( Cat # PAB10243 ) shows detection of a band at ~100 kDa ( arrowhead ) corresponding to isoform 1 of PPP1R13L in MCF-7 whole cell lysates.Preincubation with immunizing peptide blocks specific band staining ( data not shown ).Approximately 35 µg of lysate was separated by 4-20% Tris Glycine SDS-PAGE.After blocking, the membrane was probed with the primary antibody diluted to 1:1,500 in 5% BLOTTO/PBS overnight at 4°C.The membrane was washed and reacted with a 1:10,000 dilution of IRDye^TM800 conjugated Gt-a-Rabbit IgG [H&L] for 45 min at room temperature ( 800 nm channel, green ).Molecular weight estimation was made by comparison to prestained MW markers.IRDye^TM800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR.IRDye is a trademark of LI-COR, Inc.

PAB10243

Transfected 293T; WB Suggested Anti-PPP1R13L Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: Transfected 293T; PPP1R13L antibody - C-terminal region (ARP32825_P050) in Transfected 293T cells using Western Blot

ARP32825_P050

Human Lung; WB Suggested Anti-PPP1R13L Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Human Lung; PPP1R13L antibody - middle region (ARP38847_P050) in Human Lung cells using Western Blot

ARP38847_P050

WB using Anti-iASPP antibody shows detection of a band at ~100 kDa (arrowhead) corresponding to isoform 1 of iASPP in MCF7 whole cell lysates. Preincubation with immunizing peptide blocks specific band staining (data not shown). Approximately 35 ug of lysate was separated by SDS-PAGE. After blocking, the membrane was probed with the primary antibody diluted to 1:1,500. The membrane was washed and reacted with a 1:10,000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L].

TA319363

Affinity Purified anti-iASPP antibody shows strong cytoplasmic and membranous staining of myocytes in human heart tissue. Tissue was formalin-fixed and paraffin embedded. Brown color indicates presence of protein, blue color shows cell nuclei. Personal Communication, Kenneth Wester, www.proteinatlas.org, Uppsala, Sweden.

TA319363

Gel: 6%SDS-PAGE, Lysate: 40 µg, Lane 1-2: MCF7 cells, hela cells, Primary antibody: PPP1R13L Antibody at dilution 1/500, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 30 seconds

TA322477

The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer tissue using PPP1R13L Antibody at dilution 1/30, on the right is treated with synthetic peptide. (Original magnification: ×200)

TA322477

The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using PPP1R13L Antibody at dilution 1/30, on the right is treated with synthetic peptide. (Original magnification: ×200)

TA322477

WB Suggested Anti-PPP1R13L Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:1562500; Positive Control: Transfected 293T

TA331181

WB Suggested Anti-PPP1R13L Antibody Titration: 0.2-1 ug/ml; Positive Control: Transfected 293T

TA331182

Lanes: 1. 30 ug MiaPaca-2 cell lysate; 2. 30 ug Panc-1 cell lysate; Primary Antibody Dilution: ; 1:1000; Secondary Antibody: ; Goat anti-Rabbit HRP; Secondary Antibody Dilution: ; 1:4000; Gene Name: ; PPP1R13L; Submitted by: ; Dr. Crissy Dudgeon, Ph.D., C

TA331182

Sample Type : Human lung adenocarcinoma cell line A549Primary Antibody Dilution : 1:100Secondary Antibody : Goat anti-rabbit AlexaFluor 488Secondary Antibody Dilution : 1:400Color/Signal Descriptions : PPP1R13L: Green DAPI:BlueGene Name : PPP1R13L Submitt

TA331182

WB Suggested Anti-PPP1R13L Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 312500; Positive Control: Human Lung

TA343630

Primary Antibodies

Catalog No.	Host	Iso.	Pres.	React.	Applications
TA319363	PPP1R13L (780-797) antibody
	Rabbit polyclonal anti-iASSP antibody
	Rabbit	IgG	Aff - Purified	Can, Hu, Ms	E, P, WB	0.1 mg / €515.00
						OriGene Technologies, Inc.
+1 additional image
TA322477	PPP1R13L antibody
	Anti-PPP1R13L Rabbit Polyclonal Antibody
	Rabbit	IgG	Purified	Hu, Ms, Rt	E, P, WB	0.1 ml / €335.00
						OriGene Technologies, Inc.
+2 additional images
AP05685PU-N	PPP1R13L antibody
	Rabbit	IgG	Purified	Can, Hu, Ms	E, WB	50 µg / €410.00
						OriGene Technologies GmbH
AP07622PU-N	PPP1R13L antibody
	Rabbit		Aff - Purified	Hu	E, ICC/IF, P, WB	50 µg / €425.00
						OriGene Technologies GmbH

Mouse Lenti ORF Particles

Catalog No.
MR216532L2V	Lenti ORF particles, Ppp1r13l (GFP-tagged) - Mouse protein phosphatase 1, regulatory (inhibitor) subunit 13 like (Ppp1r13l), 200 uL, >10^7 TU/mL
		200 µl / €1,640.00
		OriGene Technologies, Inc.

MR216532L1V	Lenti ORF particles, Ppp1r13l (Myc-DDK-tagged) - Mouse protein phosphatase 1, regulatory (inhibitor) subunit 13 like (Ppp1r13l), 200 uL, >10^7 TU/mL
		200 µl / €1,470.00
		OriGene Technologies, Inc.

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