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PPP2R1A antibody

Principal name

PPP2R1A antibody

Alternative names for PPP2R1A antibody

Protein phosphatase 2A, PP2A subunit A PR65-alpha isoform, PP2A subunit A R1-alpha isoform, Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform

SwissProt ID

P30153 (Human), P54612 (Pig), Q76MZ3 (Mouse)

Gene ID

5518 (PPP2R1A), 51792 (Ppp2r1a)

Ncbi ID

9606, NP_055040, NP_055040.2, NP_058587.1, NM_016891, NM_014225

Available reactivities

Bov (Bovine), Hu (Human), Ms (Mouse), Rt (Rat), Rb (Rabbit), Por (Porcine), Ze (Zebrafish), African clawed frog, Can (Canine), Eq (Equine), GP (Guinea Pig), Frog

Available hosts

Rabbit, Sheep, Goat, Mouse, Rat

Available applications

Frozen Sections (C), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Paraffin Sections (P)

Background of PPP2R1A antibody

PPP2R1A is a constant regulatory subunit of protein phosphatase 2. Protein phosphatase 2 is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The constant regulatory subunit A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit. PPP2R1A is an alpha isoform of the constant regulatory subunit A.This gene encodes a constant regulatory subunit of protein phosphatase 2. Protein phosphatase 2 is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The constant regulatory subunit A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit. This gene encodes an alpha isoform of the constant regulatory subunit A. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

General readings

Hong Y, Sarge KD. Regulation of protein phosphatase 2A activity by heat shock transcription factor 2. J Biol Chem. 1999 May 7;274(19):12967-70.

Western Blot analysis: AP05040PU-N PPP2R1A antibody staining of total Rat brain homogenate.

AP05040PU-N

PPP2R1A polyclonal antibody ( Cat # PAB6772 ) ( 0.03 µg/ml ) staining of human lymph node lysate ( 35 µg protein in RIPA buffer ) . Primary incubation was 1 hour. Detected by chemiluminescence.

PAB6772

Proximity Ligation Analysis (PLA) of protein-protein interactions between TP53 and PPP2R1A HeLa cells were stained with anti-TP53 rabbit purified polyclonal 1:1200 and anti-PPP2R1A mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.

H00005518-M02

PPP2R1A monoclonal antibody (M02), clone 4E6. Western Blot analysis of PPP2R1A expression in Jurkat(Cat # L017V1 ).

H00005518-M02

Western Blot analysis of PPP2R1A expression in transfected 293T cell line (H00005518-T02) by PPP2R1A MaxPab polyclonal antibody.Lane 1: PPP2R1A transfected lysate(65.30 KDa).Lane 2: Non-transfected lysate.

H00005518-D01P

PPP2R1A polyclonal antibody ( Cat # PAB10416 ) in western blot of total rat brain homogenate. (+) indicates blocking by specific peptide.

PAB10416

PPP2R1A polyclonal antibody ( Cat # PAB10417 ) in western blot of total rat brain homogenate.

PAB10417

Human 293T; WB Suggested Anti-PPP2R1A Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: 293T cell lysate; PPP2R1A antibody - N-terminal region (ARP56741_P050) in Human 293T cells using Western Blot

ARP56741_P050

Human THP-1; WB Suggested Anti-PPP2R1A Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: THP-1 cell lysate; PPP2R1A antibody - middle region (ARP56742_P050) in Human THP-1 cells using Western Blot

ARP56742_P050

Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE)

AM31921PU-N

Western Blot analysis of PPP2R1A expression in Jurkat cell lysate using PPP2R1A monoclonal antibody, clone 4E6.

AM31921PU-N

Detection limit for recombinant GST tagged PPP2R1A is 3 ng/ml as a capture antibody.

AM31921PU-N

TP300056

(0.03ug/ml) staining of Human Lymph Node lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

TA311413

Anti-PPP2R1A Antibody (3.8µg/ml) staining of paraffin embedded Human Thyroid Gland. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

TA311413

Western blot analysis of extracts of various cell lines, using PPP2R1A antibody.

TA332922

Host: Rabbit; Target Name: PPP2R1A; Sample Tissue: HT1080 Whole cell lysates; Antibody Dilution: 1.0 ug/ml

TA344605

WB Suggested Anti-PPP2R1A Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:1562500; Positive Control: THP-1 cell lysate

TA345117

Western Blot showing PP2A Alpha Subunit (6F9) specificity: Lane 1: HeLa cell lysate (10 ug); Lane2: HeLa cell lysate (20 ug); Lane 3: BSA negative control

TA352261

Staining of PP2A Alpha Subunit (5H4) on formalin fixed paraffin embedded human liver at 1:500 dilution.

TA352202

Primary Antibodies

Catalog No.	Host	Iso.	Clone	Pres.	React.	Applications
TA311413	PPP2R1A (C-term) antibody
	Goat Anti-PP2A / PPP2R1A Antibody
	Goat			Aff - Purified	Bov, Can, Hu, Ms, Por, Rt	P, WB	0.1 mg / €360.00
							OriGene Technologies, Inc.
+1 additional image
TA332922	PPP2R1A antibody
	Rabbit anti-PPP2R1A Polyclonal Antibody
	Rabbit	IgG		Purified	Hu, Ms, Rt		0.1 mg / €335.00
							OriGene Technologies, Inc.
AM31921PU-N	PPP2R1A antibody
	Mouse	IgG1	4E6	Purified	Hu	E, P, WB	50 µg / €475.00
							OriGene Technologies GmbH
+2 additional images
AP05039PU-N	PPP2R1A antibody
	Rabbit	IgG		Purified	Bov, Hu, Ms, Rt	C, WB	0.1 mg / €400.00
							OriGene Technologies GmbH
AP05040PU-N	PPP2R1A antibody
	Sheep	IgG		Purified	Bov, Hu, Ms, Rt	C, WB	0.1 mg / €400.00
							OriGene Technologies GmbH

Proteins & Growth Factors

Catalog No.	Species	Purity	Source
TP300056	PPP2R1A
	Recombinant protein of human protein phosphatase 2 (formerly 2A), regulatory subunit A, alpha isoform (PPP2R1A)
	Human	> 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.	HEK293 cells	20 µg / €748.00
				OriGene Technologies, Inc.

Mouse Lenti ORF Particles

Catalog No.
MR218366L2V	Lenti ORF particles, Ppp2r1a (GFP-tagged) - Mouse protein phosphatase 2 (formerly 2A), regulatory subunit A (PR 65), alpha isoform (Ppp2r1a), 200 uL, >10^7 TU/mL
		200 µl / €1,320.00
		OriGene Technologies, Inc.

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