PPP2R5D antibody
Principal name
PPP2R5D antibody
Alternative names for PPP2R5D antibody
Protein phosphatase 2A, PP2A B subunit B'delta isoform, PP2A B subunit B56 delta isoform, PP2A B subunit PR61 delta isoform, PP2A B subunit R5 delta isoform, Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit delta isoform
Available reactivities
Available hosts
Available applications
Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P)
Background of PPP2R5D antibody
Protein phosphatase 2A is a heterotrimeric protein serine/threonine phosphatase consisting of a 36-kD catalytic C subunit (alpha and beta isoforms), a 65-kD structural A subunit (alpha and beta isoforms), and a variable regulatory B subunit. When the PP2A catalytic C subunit associates with the A and B subunits several species of holoenzymes are produced with distinct functions and characteristics. Ubiquitously expressed, PP2A is involved in a broad range of cellular processes, including membrane receptor desensitization, signal transduction, intermediary metabolism, transcriptional regulation and control of DNA replication, and mitosis. The PP2A phosphatase family accounts for the majority of Ser/Thr phosphatase activity in the brain and specifically regulates tau phosphorylation, both directly as tau phosphatase and indirectly by regulating the activities of several tau kinases which include CaM Kinase II, PKA, MAP kinase kinase (MEK 1/2), extracellular regulated kinase (ERK 1/2), GSK-3 beta;, and P70S6 kinase, thus regulating tau microtubule binding in the brain. When PP2A B' delta (PP2A 56 kDa regulatory subunit delta isoform) is associated with the A and C subunits, tau protein cannot bind to PP2A and be dephosphorylated, leading to increased tau aggregation.
General readings
McCright B, Virshup DM.
Identification of a new family of protein phosphatase 2A regulatory subunits.
J Biol Chem 1995 Nov 3;270(44):26123-8
PMID: 7592815
PPP2R5D polyclonal antibody ( Cat # PAB6072 ) staining ( 2 µg/ml ) of 293 lysate ( RIPA buffer, 30 µg total protein per lane ) . Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Western Blot analysis of PPP2R5D expression in transfected 293T cell line by PPP2R5D monoclonal antibody (M01), clone 4E12.Lane 1: PPP2R5D transfected lysate(70 KDa).Lane 2: Non-transfected lysate.
PPP2R5D monoclonal antibody (M21), clone 1A3. Western Blot analysis of PPP2R5D expression in Jurkat(Cat # L017V1 ).
Human 721_B; WB Suggested Anti-SERPINA3 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: 721_B cell lysate; SERPINA3 antibody - middle region (ARP51880_P050) in Human 721_B cells using Western Blot
Human COLO205; WB Suggested Anti-PPP2R5D Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: COLO205 cell lysate; PPP2R5D antibody - middle region (ARP57773_P050) in Human COLO205 cells using Western Blot
Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE)
Western blot of extracts from Jurkat/HUVEC cells, using PPP2R5D Antibody. The lane on the right is treated with the synthesized peptide.
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-PPP2R5D mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA803916)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-PPP2R5D mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA803916)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-PPP2R5D mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA803916)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-PPP2R5D mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA803916)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PPP2R5D (RC201945, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PPP2R5D.
Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-PPP2R5D mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA804105)
Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-PPP2R5D mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA804105)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-PPP2R5D mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA804105)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PPP2R5D (RC201945, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PPP2R5D.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PPP2R5D (RC201945, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PPP2R5D.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PPP2R5D (RC201945, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PPP2R5D.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PPP2R5D (RC201945, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PPP2R5D.
Immunohistochemical analysis of PPP2R5D staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugad compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Western blot analysis of PPP2R5D expression in SHSY5Y (A); HuvEc (B); HEK293T (C) whole cell lysates.
TA302740 staining (2µg/ml) of 293 lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Western blot analysis of extracts from HuvEc cells and Jurkat cells, using PPP2R5D antibody.The lane on the right is treated with the synthesized peptide.
WB Suggested Anti-PPP2R5D Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:62500; Positive Control: COLO205 cell lysate
Western blot showing PP2A regulatory, subunit B56 delta. Lane 1: Rat brain lysate (25ug) clone H5-D12 was used at 1 ug/mL.
- Quick-Filters:
- Highly Validated (2)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA803916 | PPP2R5D antibody |
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| PPP2R5D mouse monoclonal antibody, clone OTI5E7 (formerly 5E7) | |||||||
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Mouse | IgG2b | OTI5E7 | Purified | Hu | P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
 +4 additional images |
Highly validated antibody!
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| TA804105 | PPP2R5D antibody |
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| PPP2R5D mouse monoclonal antibody, clone OTI3B3 (formerly 3B3) | |||||||
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Mouse | IgG1 | OTI3B3 | Purified | Hu | P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +3 additional images |
Highly validated antibody!
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| TA302740 | PPP2R5D (C-term) antibody |
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| Goat Polyclonal Antibody against PPP2R5D | |||||||
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Goat | Aff - Purified | Bov, Can, Hu, Ms, Por, Rt | WB |
0.1 mg /
€360.00
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| OriGene Technologies, Inc. | |||||||
| TA312818 | PPP2R5D antibody |
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| Rabbit polyclonal anti-PPP2R5D antibody | |||||||
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Rabbit | IgG | Purified | Hu, Ms | E, WB |
0.1 ml /
€335.00
|
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| OriGene Technologies, Inc. | |||||||
| TA804106 | PPP2R5D antibody |
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| PPP2R5D mouse monoclonal antibody, clone OTI6C12 (formerly 6C12) | |||||||
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Mouse | IgG2a | OTI6C12 | Purified | Hu | WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
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| TA804123 | PPP2R5D antibody |
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| PPP2R5D mouse monoclonal antibody, clone OTI10D11 (formerly 10D11) | |||||||
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Mouse | IgG1 | OTI10D11 | Purified | Hu | WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
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| TA804124 | PPP2R5D antibody |
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| PPP2R5D mouse monoclonal antibody, clone OTI2F2 (formerly 2F2) | |||||||
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Mouse | IgG1 | OTI2F2 | Purified | Hu | WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
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| AP05044PU-N | PPP2R5D antibody |
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Rabbit | IgG | Purified | Bov, Hu, Ms, Rt | WB |
0.1 mg /
€400.00
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| OriGene Technologies GmbH | |||||||
| AP33283PU-N | PPP2R5D (544-593) antibody |
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Rabbit | IgG | Aff - Purified | Hu, Ms, Rt | E, P, WB |
50 µl /
€425.00
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| OriGene Technologies GmbH | |||||||
| +1 additional image |
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Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP300647 | PPP2R5D (transcript variant 2) |
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| Recombinant protein of human protein phosphatase 2, regulatory subunit B', delta isoform (PPP2R5D), transcript variant 2 | |||||
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Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
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| OriGene Technologies, Inc. | |||||
| TP301945 | PPP2R5D (transcript variant 1) |
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| Purified recombinant protein of Homo sapiens protein phosphatase 2, regulatory subunit B', delta isoform (PPP2R5D), transcript variant 1 | |||||
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Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
| TP760026 | PPP2R5D (full length, N-term HIS tag, transcript variant 1) |
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| Recombinant protein of human protein phosphatase 2, regulatory subunit B', delta (PPP2R5D), transcript variant 1, full length, with N-terminal HIS tag, expressed in E.Coli, 50ug | |||||
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Human | > 80 % Preparation: . Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
E. coli |
50 µg /
€205.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY405833 | PPP2R5D overexpression lysate |
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0.1 mg /
€295.00
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| OriGene Technologies, Inc. | ||
| LY405834 | PPP2R5D overexpression lysate |
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0.1 mg /
€495.00
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| OriGene Technologies, Inc. | ||
| LY430509 | PPP2R5D overexpression lysate |
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0.1 mg /
€315.00
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| OriGene Technologies, Inc. |