PPP4C antibody
Principal name
PPP4C antibody
Alternative names for PPP4C antibody
PPP4, PP4C, PPX, PP-X, Protein phosphatase X, Serine/threonine-protein phosphatase 4 catalytic subunit
SwissProt ID
A6H772 (Bovin), O76932 (Drome), P11084 (Rabit), P32345 (Yeast), P60510 (Human), P97470 (Mouse), Q5BJ92 (Rat), Q6IP91 (Xenla)
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Frozen Sections (C), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF)
Background of PPP4C antibody
- Kit Component:
- - KN200538G1, PPP4C gRNA vector 1 in pCas-Guide vector
- KN200538G2, PPP4C gRNA vector 2 in pCas-Guide vector
- KN200538D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Cohen P T., et al. (1993) Biochem Soc Trans. 21:884-888.Mumby M C., et al. (1993) Phys Rev. 73:673-699.
PP X/C antibody western blot of total bovine testes homogenate. (+) indicates presence of specific blocking peptide of control peptide (-).
PP X/C interior antibody western blot of total bovine testes homogenate. (+) indicates presence of specific blocking peptide of control peptide (-).
Figure 2. Staining PPP4C in Prostate by Immunohistochemistry using Formalin-Fixed Paraffin-Embedded (FFPE) tissue.
Figure 1. Staining PPP4C in Brain, Cortex by Immunohistochemistry using Formalin-Fixed Paraffin-Embedded (FFPE) tissue.
PPP4C polyclonal antibody ( Cat # PAB14146 ) ( 0.3µg/ml ) staining of HEK293 lysate ( 35µg protein in RIPA buffer ). Primary incubation was 1 hour. Detected by chemiluminescence.
Human Muscle; WB Suggested Anti-PPP4C Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: Human Muscle; PPP4C antibody - middle region (ARP58516_P050) in Human Muscle cells using Western Blot
Human Small Intestine (formalin-fixed, paraffin-embedded) stained with PPP4C antibody AP22586PU-N followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.
Human Prostate (formalin-fixed, paraffin-embedded) stained with PPP4C antibody AP22586PU-N followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.
Antibody (0.3 µg/ml) staining of HEK293 lysate (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
TA305920 (0.3 ug/ml) staining of Rat Spleen (A) and Lung (B) lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
TA305920 (2.5 ug/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Western blot analysis of extracts of various cell lines, using PPP4C antibody.
Immunohistochemistry of paraffin-embedded human lung cancer using PPP4C antibody at dilution of 1:200 (400x lens).
Immunohistochemistry of paraffin-embedded human stomach cancer using PPP4C antibody at dilution of 1:200 (400x lens).
Immunohistochemistry of paraffin-embedded rat lung using PPP4C antibody at dilution of 1:200 (400x lens).
Immunohistochemistry of paraffin-embedded rat spleen using PPP4C antibody at dilution of 1:200 (400x lens).
Immunofluorescence analysis of MCF7 cell using PPP4C antibody. Blue: DAPI for nuclear staining.
WB Suggested Anti-PPP4C Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:1562500; Positive Control: Human Muscle
Western blot detection of Protein Phosphatase 4C in Hela and Jurkat cell lysates using Protein Phosphatase 4C mouse mAb (1:200 diluted).Predicted band size: 34KDa.Observed band size: 34KDa.
IHC of paraffin-embedded human breast cancer using anti-Protein Phosphatase 4C mouse mAb diluted 1/500-1/1000.
Immunocytochemistry of HeLa cells using anti-Protein Phosphatase 4C mouse mAb diluted 1:200.
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| AP05056PU-N | PPP4C antibody |
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Rabbit | IgG | Purified | Bov, Hu, Ms, Rt | C, WB |
0.1 mg /
€400.00
|
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| OriGene Technologies GmbH | |||||||
| AP05057PU-N | PPP4C antibody |
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 |
Sheep | IgG | Purified | Bov, Hu, Ms, Rt | C, WB |
0.1 mg /
€400.00
|
|
| OriGene Technologies GmbH | |||||||