PRKAR1A antibody
Principal name
PRKAR1A antibody
Alternative names for PRKAR1A antibody
PKR1, PRKAR1, TSE1, CAR, CNC1, cAMP-dependent protein kinase type I-alpha regulatory subunit, Tissue-specific extinguisher 1
SwissProt ID
P00514 (Bovin), P07802 (Pig), P09456 (Rat), P10644 (Human), Q5ZM91 (Chick), Q9DBC7 (Mouse)
Ncbi ID
9606, NP_002725.1, NP_002725, NP_997636, NP_997637, NP_068680.1, NM_021880, NM_013181, NM_212471, NM_212472
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF)
Background of PRKAR1A antibody
The second messenger cyclic AMP (cAMP) activates cAMP-dependent protein kise (PKA or cAPK) in mammalian cells and controls many cellular mechanisms such as gene transcription, ion transport, and protein phosphorylation. Ictive PKA is a heterotetramer composed of a regulatory subunit (R) dimer and a catalytic subunit (C) dimer. In this ictive state, the pseudosubstrate sequences on the R subunits block the active sites on the C subunits. Three C subunit isoforms (C-a, C-ß, and C-?) and two families of regulatory subunits (RI and RII) with distinct cAMP binding properties have been identified. The two R families exist in two isoforms, a and ß (RI-a, RI-ß, RII-a, and RII-ß). Upon binding of cAMP to the R subunits, the autoinhibitory contact is eased and active monomeric C subunits are released. PKA shares substrate specificity with Akt (PKB) and PKC, which are characterized by an arginine at position -3 relative to the phosphorylated serine or threonine residue. Substrates that present this consensus sequence and have been shown to be phosphorylated by PKA are Bad (Ser155), CREB (Ser133), and GSK-3 (GSK-3a Ser21 and GSK-3ß Ser9). In addition, combined knock-down of PKA C-a and -ß blocks cAMP-mediated phosphorylation of Raf (Ser43 and Ser259). Autophosphorylation and phosphorylation by PDK-1 are two known mechanisms responsible for phosphorylation of the C subunit at Thr197.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with PRKAR1A polyclonal antibody ( Cat # PAB3313 ) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. HC = hepatocarcinoma.
Western blot analysis of PRKAR1A polyclonal antibody ( Cat # PAB3313 ) in Jurkat cell lysate. PRKAR1A ( arrow ) was detected using purified polyclonal antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
Western Blot analysis of PRKAR1A expression in transfected 293T cell line (H00005573-T01) by PRKAR1A MaxPab polyclonal antibody.Lane 1: PRKAR1A transfected lysate(43.00 KDa).Lane 2: Non-transfected lysate.
Western blot (WB) analysis of PKR1 antibody (Cat.-No.: AP01335PU-N) in extracts from COLO cells.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining
Western blot analysis of anti-PKR1 Antibody (N-term) in CEM cell line lysates (35ug/lane). PKR1 (arrow) was detected using the purified Pab.
Western Blot analysis of PRKAR1A expression in transfected 293T cell line (H00005573-T02) by PRKAR1A MaxPab polyclonal antibody.Lane 1: PRKAR1A transfected lysate(41.91 KDa).Lane 2: Non-transfected lysate.
Human Liver; WB Suggested Anti-PRKAR1A Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: Human Liver; PRKAR1A antibody - C-terminal region (ARP57832_P050) in Human Liver cells using Western Blot
Immunofluorescent analysis of PRKAR1A staining in HT29 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Western blot analysis of PRKAR1A expression in Jurkat (A); HT29 (B); H1299 (C); A549 (D) whole cell lysates.
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-PRKAR1A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500641, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded Carcinoma of bladder tissue using anti-PRKAR1Amouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500641, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded endometrium tissue within the normal limits using anti-PRKAR1Amouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500641, Dilution 1:50)
Figure from citation: PRKAR1A IHC in a CNC-associated cardiac myxoma. A, A photomicrograph of the specimen at low power exhibits the robust reactivity of the adjacent normal myocardium with antibodies directed against PRKAR1A. B, A high-power photomicrograph exhibits the lack of reactivity seen in the neoplastic (myxoma) cells, although the intratumoral histiocytes are still strongly reactive. Dilution: 1:8000 View Citation
Immunohistochemical staining of paraffin-embedded colon tissue within the normal limits using anti-PRKAR1Amouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500641, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded prostate tissue within the normal limits using anti-PRKAR1Amouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500641, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded Ovary tissue within the normal limits using anti-PRKAR1Amouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500641, Dilution 1:50)
Anti-PRKAR1A mouse monoclonal antibody (TA500641) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PRKAR1A(RC212810).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PRKAR1A (RC212810, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PRKAR1A.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PRKAR1A monoclonal antibody.
Immunohistochemical staining of human testis shows strong cytoplasmic positivity in cells of the seminiferus ducts.This validation was performed by Protein Atlas and the presentation of data is for informational purposes only.
Immunofluorescent staining of human cell line A-431 shows positivity in cytoplasm.This validation was performed by Protein Atlas and the presentation of data is for informational purposes only.
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11; Lane 2: RT-4; Lane 3: U-251 MG; Lane 4: Human Plasma; Lane 5: Liver; Lane 6: TonsilThis validation was performed by Protein Atlas and the presentation of data is for informational purposes only.
ELISA: Protein Kinase A regulatory subunit I alpha Antibody
Western blot analysis of extracts from HepG2 cells, using KAP0 antibody.The lane on the right is treated with the synthesized peptide.
Predicted band size: 43 kDa . Positive control: HeLa, CEM, A549, SW480, PC3, V251, HCTnb and heart tissue lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lane1: HeLa cell lysate Lane2:CEM cell lysate Lane3: A549 cell lysate Lane4: SW480 cell lysate Lane5: PC3 cell lysate Lane6: V251 cell lysate Lane7: HCTnb cell lysate Lane8: Heart tissue lysate Lysates: 40 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution)
Western blot analysis of extracts of various cell lines using PRKAR1A antibody.
Immunohistochemistry of paraffin-embedded human esophageal cancer using PRKAR1A antibody at dilution of 1:100 (200x lens).
Immunohistochemistry of paraffin-embedded human esophagus using PRKAR1A antibody at dilution of 1:100 (200x lens).
Immunofluorescence analysis of HeLa cell using PRKAR1A antibody. Blue: DAPI for nuclear staining.
WB Suggested Anti-PRKAR1A Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:62500; Positive Control: Human Liver
WB Suggested Anti-Prkar1a Antibody; Titration: 1.0 ug/ml; Positive Control: Rat Spleen
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Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA500641 | PRKAR1A antibody |
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| anti-PRKAR1A (PKA regulatory subunit I alpha) mouse monoclonal antibody, clone OTI6C7 (formerly 6C7) | |||||||
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Mouse | IgG1 | OTI6C7 | Purified | Hu | ICC/IF, P, WB |
0.1 ml /
€379.00
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| OriGene Technologies, Inc. | |||||||
 +9 additional images |
Top Selling product! Published recently! Highly validated antibody!
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| TA326861 | PRKAR1A antibody |
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| Rabbit anti-PRKAR1A Polyclonal Antibody | |||||||
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Rabbit | IgG | Purified | Hu, Ms, Rt |
0.1 mg /
€335.00
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| OriGene Technologies, Inc. | |||||||
| +3 additional images |
Highly validated antibody! |
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| TA312524 | PRKAR1A antibody |
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| Rabbit polyclonal anti-KAP0 antibody | |||||||
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Rabbit | IgG | Purified | Hu, Ms, Rt | E, WB |
0.1 ml /
€335.00
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| OriGene Technologies, Inc. | |||||||
| TA324281 | PRKAR1A antibody |
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| Anti-PRKAR1A Rabbit Polyclonal Antibody | |||||||
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Rabbit | IgG | Purified | Hu, Ms, Rt | WB |
0.1 ml /
€335.00
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| OriGene Technologies, Inc. | |||||||
| AP01335PU-N | PRKAR1A antibody |
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Rabbit | Aff - Purified | Hu, Ms, Rt | ICC/IF, WB |
0.1 mg /
€370.00
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| OriGene Technologies GmbH | |||||||
| AP15053PU-N | PRKAR1A (N-term) antibody |
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Rabbit | Ig | Purified | Hu | P, WB |
0.4 ml /
€415.00
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| OriGene Technologies GmbH | |||||||
| +1 additional image |
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Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP303828 | PRKAR1A (transcript variant 1) |
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| Recombinant protein of human protein kinase, cAMP-dependent, regulatory, type I, alpha (tissue specific extinguisher 1) (PRKAR1A), transcript variant 1 | |||||
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Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
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| OriGene Technologies, Inc. | |||||
| TP310623 | PRKAR1A (transcript variant 2) |
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| Recombinant protein of human protein kinase, cAMP-dependent, regulatory, type I, alpha (tissue specific extinguisher 1) (PRKAR1A), transcript variant 2 | |||||
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Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
| TP312810 | PRKAR1A (transcript variant 3) |
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| Recombinant protein of human protein kinase, cAMP-dependent, regulatory, type I, alpha (tissue specific extinguisher 1) (PRKAR1A), transcript variant 3 | |||||
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Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
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| OriGene Technologies, Inc. | |||||
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR205941L2V | Lenti ORF particles, Prkar1a (GFP-tagged) - Mouse protein kinase, cAMP dependent regulatory, type I, alpha (Prkar1a), 200 uL, >10^7 TU/mL |
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200 µl /
€930.00
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| OriGene Technologies, Inc. | ||
| MR205941L1V | Lenti ORF particles, Prkar1a (Myc-DDK-tagged) - Mouse protein kinase, cAMP dependent regulatory, type I, alpha (Prkar1a), 200 uL, >10^7 TU/mL |
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200 µl /
€830.00
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| OriGene Technologies, Inc. | ||
Rat Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RR209845L2V | Lenti ORF particles, Prkar1a (GFP-tagged ORF) - Rat protein kinase, cAMP dependent regulatory, type I, alpha (Prkar1a), 200 uL, >10^7 TU/mL |
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200 µl /
€930.00
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| OriGene Technologies, Inc. | ||
| RR209845L1V | Lenti ORF particles, Prkar1a (Myc-DDK-tagged ORF) - Rat protein kinase, cAMP dependent regulatory, type I, alpha (Prkar1a), 200 uL, >10^7 TU/mL |
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200 µl /
€830.00
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| OriGene Technologies, Inc. | ||