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PRKCBP1 antibody

Principal name

PRKCBP1 antibody

Alternative names for PRKCBP1 antibody

Protein kinase C-binding protein 1, RACK7, ZMYND8, KIAA1125, Cutaneous T-cell lymphoma-associated antigen se14-3, CTCL-associated antigen se14-3, Zinc finger MYND domain-containing protein 8

SwissProt ID

Q9H1F4 (Human), Q9ULU4 (Human)

Gene ID

23613 (ZMYND8)

Ncbi ID

9606, NP_898868, NP_036540.3, NM_183047, NM_183048, NM_012408

Available reactivities

Hu (Human), Can (Canine), Ms (Mouse), Rt (Rat), Ze (Zebrafish), African clawed frog, Bov (Bovine), Eq (Equine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of PRKCBP1 antibody

ZMYND8 is a receptor for activated C-kise (RACK) protein. ZMYND8 has been shown to bind in vitro to activated protein kise C beta I. In addition, this protein is a cutaneous T-cell lymphoma-associated antigen. Filly, the protein contains a bromodomain and two zinc fingers, and is thought to be a transcriptiol regulator.The protein encoded by this gene is a receptor for activated C-kise (RACK) protein. The encoded protein has been shown to bind in vitro to activated protein kise C beta I. In addition, this protein is a cutaneous T-cell lymphoma-associated antigen. Filly, the protein contains a bromodomain and two zinc fingers, and is thought to be a transcriptiol regulator. Multiple transcript variants encoding several different isoforms have been found for this gene.

Immunoperoxidase of monoclonal antibody to ZMYND8 on formalin-fixed paraffin-embedded human endometrium. [antibody concentration 0.25 ug/ml]

H00023613-M01

PRKCBP1 monoclonal antibody (M01), clone 5B12 Western Blot analysis of PRKCBP1 expression in Hela NE ( Cat # L013V3 ).

H00023613-M01

Detection limit for recombinant GST tagged PRKCBP1 is approximately 0.03ng/ml as a capture antibody.

H00023613-M01

PRKCBP1 monoclonal antibody (M06), clone 6A12. Western Blot analysis of PRKCBP1 expression in Hela NE ( Cat # L013V3 ).

H00023613-M06

Nuclear extracts from HeLa cells ( 40 µg ) were analysed by Western blot using ZMYND8 polyclonal antibody ( Cat # PAB14097 ) diluted 1 : 1,000 in TBS-Tween containing 5% skimmed milk.

PAB14097

ELISA was performed using a serial dilution of ZMYND8 polyclonal antibody ( Cat # PAB14097 ). The plates were coated with the peptide used for immunization of the rabbit. By plotting the absorbance against the antibody dilution, the titer of the crude serum was estimated to be 1 : 36,000.

PAB14097

Human Brain; WB Suggested Anti-ZMYND8 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Human brain; ZMYND8 antibody - N-terminal region (ARP34729_P050) in Human Brain cells using Western Blot

ARP34729_P050

Human HeLa; WB Suggested Anti-ZMYND8 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Hela cell lysate; ZMYND8 antibody - middle region (ARP34902_P050) in Human HeLa cells using Western Blot

ARP34902_P050

Western blot analysis of extracts from 293 cells, using PKCB1 Antibody. The lane on the right is treated with the synthesized peptide.

Immunohistochemical analysis of RACK7 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugad compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Immunofluorescent analysis of RACK7 staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Western blot analysis of RACK7 expression in HeLa (A) whole cell lysates.

Western blot analysis of extracts from 293 cells, using PKCB1 antibody.The lane on the right is treated with the synthesized peptide.

TA315262

WB Suggested Anti-ZMYND8 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: Human brain

TA337717

WB Suggested Anti-ZMYND8 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: Hela cell lysate

TA337718

WB using the antibody against ZMYND8 diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right (expected size: 132 kDa); the marker (in kDa) is shown on the left.

TA347318

Determination of the titer To determine the titer, an ELISA was performed using a serial dilution of the antibody against human ZMYND8. The plates were coated with the peptide used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:36,000.

TA347318

Western blot analysis on 293 cell lysate using PKCB1 Antibody

TA347629

Primary Antibodies

Catalog No.	Host	Iso.	Pres.	React.	Applications
TA315262	PRKCBP1 antibody
	Rabbit polyclonal anti-PKCB1 (PKC Binding Protein 1) antibody
	Rabbit	IgG	Purified	Hu	E, WB	0.1 ml / €335.00
						OriGene Technologies, Inc.
TA347318	PRKCBP1 antibody
	Rabbit Polyclonal ZMYND8 Antibody
	Rabbit	IgG	Purified	Hu	E	0.1 ml / €285.00
						OriGene Technologies, Inc.
+1 additional image
TA347629	PRKCBP1 antibody
	Rabbit Polyclonal Anti-PKCB1 Antibody
	Rabbit	IgG	Purified	Hu	WB	0.1 mg / €335.00
						OriGene Technologies, Inc.

Human Lenti ORF Particles

Catalog No.
RC212828L2V	Lenti ORF particles, ZMYND8 (mGFP-tagged)-Human zinc finger, MYND-type containing 8 (ZMYND8), transcript variant 1, 200 uL, >10^7 TU/mL
		200 µl / €2,480.00
		OriGene Technologies, Inc.

RC221394L2V	Lenti ORF particles, ZMYND8 (mGFP-tagged)-Human zinc finger, MYND-type containing 8 (ZMYND8), transcript variant 2, 200 uL, >10^7 TU/mL
		200 µl / €2,430.00
		OriGene Technologies, Inc.

RC215969L2V	Lenti ORF particles, ZMYND8 (mGFP-tagged)-Human zinc finger, MYND-type containing 8 (ZMYND8), transcript variant 3, 200 uL, >10^7 TU/mL
		200 µl / €2,390.00
		OriGene Technologies, Inc.

RC212828L1V	Lenti ORF particles, ZMYND8 (Myc-DDK-tagged)-Human zinc finger, MYND-type containing 8 (ZMYND8), transcript variant 1, 200 uL, >10^7 TU/mL
		200 µl / €2,240.00
		OriGene Technologies, Inc.

RC221394L1V	Lenti ORF particles, ZMYND8 (Myc-DDK-tagged)-Human zinc finger, MYND-type containing 8 (ZMYND8), transcript variant 2, 200 uL, >10^7 TU/mL
		200 µl / €2,200.00
		OriGene Technologies, Inc.

RC215969L1V	Lenti ORF particles, ZMYND8 (Myc-DDK-tagged)-Human zinc finger, MYND-type containing 8 (ZMYND8), transcript variant 3, 200 uL, >10^7 TU/mL
		200 µl / €2,150.00
		OriGene Technologies, Inc.

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