PRKCG antibody
Principal name
PRKCG antibody
Alternative names for PRKCG antibody
PKC-gamma, PKC gamma, PKCG, PKCC, Protein kinase C gamma type
Ncbi ID
9606, NP_002730, NP_002730.1, 10116, NM_002739, NM_002739.3, NP_036760.1, NP_035232.1, NM_011102, NM_012628
Available reactivities
Available hosts
Available applications
Paraffin Sections (P), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Immunoprecipitation (IP), Immunocytochemistry/Immunofluorescence (ICC/IF), Frozen Sections (C), Flow Cytometry (F)
Background of PRKCG antibody
Protein kise C alpha (PKCα), also known as PRKCA, refers to both a human gene and the protein that is encoded by it. Protein kise C (PKC) is a family of serine- and threonine-specific protein kises that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular sigling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This kise has been reported to play roles in many different cellular processes, such as cell adhesion, cell transformation, cell cycle checkpoint, and cell volume control. Knockout studies in mice suggest that this kise may be a fundamental regulator of cardiac contractility and Ca2+ handling in myocytes. The purified anti-PKCα Phospho (Thr638) antibody detects PKCα only when phosphorylated at threonine 638.
Formalin-fixed and paraffin-embedded human brain tissue reacted with PKC gamma antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Western blot analysis of anti-PKC gamma C-term Pab in mouse brain lysate. PKC gamma (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
Western blot with PKC γ Polyclonal Antibody
Human Placenta; WB Suggested Anti-PRKCG Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: Human Placenta; PRKCG antibody - N-terminal region (ARP56425_P050) in Human Placenta cells using Western Blot
HCT116; Quiescent human colon carcinoma HCT116 cultures were treated with 10% FBS for three time points (0, 15, 30min) or (0, 30, 60min) were used in Matrix-ChIP and real-time PCR assays at EGR1 gene (Exon1) and 15kb upstream site.; PRKCG antibody - N-terminal region (ARP56425_P050) in HCT116 using CHIP
Human 293T; WB Suggested Anti-PRKCG Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: 293T cell lysate; PRKCG antibody - middle region (ARP56426_P050) in Human 293T cells using Western Blot
Anti-PKC-g (DB 008). Western blot of PKC-g in mouse brain crude lysate (200μg of protein loaded.
Anti-PKC-g (DB 008). Western blot of PKC-g in mouse brain crude lysate (200μg of protein loaded.
PRKCG polyclonal antibody (A01), Lot # 051122JC01 Western Blot analysis of PRKCG expression in Jurkat ( Cat # L017V1 ).
Western blot analysis of PRKCG isoforms in neonatal rat brain lysate. The rat brain blot was probed with PRKCG monoclonal antibody, clone M110 ( Cat # MAB1367 ) at decreasing dilutions : Lane 1 = 1:250. Lane 2 = 1:500. Lane 3 = 1:1000.
PRKCG monoclonal antibody, clone A12-H ( Cat # MAB1632 ) . Western blot of PRKCG in mouse brain crude lysate ( 200 µg of protein loaded ) .
Western Blot analysis of PRKCG expression from cell extracts with PRKCG polyclonal antibody ( Cat # PAB12321 ).Lane 1 : rat brain tissue lysate.Lane 2 : rat brain tissue lysate.
Formalin-fixed and paraffin-embedded human brain tissue reacted with PRKCG polyclonal antibody ( Cat # PAB4539 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining . This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated .
Western blot analysis of PRKCG polyclonal antibody ( Cat # PAB4539 ) in mouse brain lysate . PRKCG ( arrow ) was detected using purified PRKCG polyclonal antibody ( Cat # PAB4539 ) . Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence .
Western blot analysis of PKC gamma expression in HeLa (A); A431 (B); THP1 (C); mouse brain (D); rat brain (E) whole cell lysates.
Western blot analysis of extracts from A549 cells, using PRKCG antibody.The lane on the right is treated with the synthesized peptide.
Western blot analysis of extracts from Rat brain cells, using PRKCG(Ab-655) antibody.
Jurkat total cell lysates were resloved by electrophoresis, transferred to nitrocellulose and probed either with anti-phosphor-PKA (T638) antibody solely (Lane 1), anti-phosphor-PKA (T638) antibody plus phosphor-peptide (Lane 2), or with the anti-phosphor-PKA (T638) antibody plus non-phosphor-peptide (Lane 3). Proteins were visualized using an HRP donkey anti-rabbit secondary antibody and a chemiluminescence detection system.
WB Suggested Anti-PRKCG Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:1562500; Positive Control: Human Placenta
Quiescent human colon carcinoma HCT116 cultures were treated with 10% FBS for three time points (0, 15, 30min) or (0, 30, 60min) were used in Matrix-ChIP and real-time PCR assays at EGR1 gene (Exon1) and 15kb upstream site.
WB Suggested Anti-PRKCG Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: 293T cell lysate
Gel: 10%SDS-PAGE, Lysate: 40 ug, Lane: Mouse brain tissue, Primary antibody: (PRKCG Antibody) at dilution 1/500, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 20 seconds
Jurkat total cell lysates were resloved by electrophoresis, transferred to nitrocellulose and probed either with anti-phosphor-PKA (T638) antibody solely (Lane 1), anti-phosphor-PKA (T638) antibody plus phosphor-peptide (Lane 2), or with the anti-phosphor-PKA (T638) antibody plus non-phosphor-peptide (Lane 3). Proteins were visualized using an HRP donkey anti-rabbit secondary antibody and a chemiluminescence detection system.
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA313070 | PRKCG antibody |
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| Rabbit polyclonal anti-PRKCG antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms | E, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA326206 | PRKCG antibody |
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| Rabbit polyclonal PRKCG (Ab-655) antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms | WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA350905 | PRKCG antibody |
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| Rabbit Polyclonal Anti-PRKCG Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| AP13550PU-N | PRKCG (C-term) antibody |
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 |
Rabbit | Ig | Purified | Hu, Mky, Ms | P, WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies GmbH | |||||||
 +1 additional image |
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| AP23311PU-N | PRKCG (C-term) antibody |
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 |
Rabbit | IgG | Aff - Purified | Hu, Ms, Rt | WB |
0.1 mg /
€390.00
|
|
| OriGene Technologies GmbH | |||||||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP308502 | PRKCG |
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| Recombinant protein of human protein kinase C, gamma (PRKCG) | |||||
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Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY419134 | PRKCG overexpression lysate |
|
 |
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. |
Rat Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RR211929L2V | Lenti ORF particles, Prkcg (GFP-tagged ORF) - Rat protein kinase C, gamma (Prkcg), 200 uL, >10^7 TU/mL |
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200 µl /
€1,460.00
|
|
| OriGene Technologies, Inc. | ||
| RR211929L1V | Lenti ORF particles, Prkcg (Myc-DDK-tagged ORF) - Rat protein kinase C, gamma (Prkcg), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,310.00
|
|
| OriGene Technologies, Inc. | ||
