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PRMT3 antibody

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Principal name

PRMT3 antibody

Alternative names for PRMT3 antibody

HRMT1L3, Protein arginine N-methyltransferase 3, Heterogeneous nuclear ribonucleoprotein methyltransferase-like protein

SwissProt ID

O13648 (Schpo), O60678 (Human), O70467 (Rat), Q0WVD6 (Arath), Q922H1 (Mouse)

Gene ID

10196 (PRMT3), 71974 (Prmt3), 89820

Ncbi ID

NP_005779, 9606, ACE87112.1, NM_005788, NP_005779.1, NP_598501.1, BC050775, NM_133740, NM_053557, NM_001145166

Available reactivities

Bov (Bovine), Can (Canine), Hu (Human), Ms (Mouse), Rt (Rat), African clawed frog, Eq (Equine), GP (Guinea Pig), Rb (Rabbit)

Available hosts

Rabbit, Mouse, Goat

Available applications

Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P)

Background of PRMT3 antibody

Kit Component:
- KN313936G1, Prmt3 gRNA vector 1 in pCas-Guide vector
- KN313936G2, Prmt3 gRNA vector 2 in pCas-Guide vector
- KN313936D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector
CRISPR Manual:
CRISPR/CAS9 Genome Editing Manual

General readings

Tang J, Gary JD, et al. (1998). J Biol Chem. 273(27):16935-45.Choi S, Jung CR, et al. (2008). Biochim Biophys Acta. 1780(9):1062-9.

ARP32141_P050 - PRMT3
Human HepG2; WB Suggested Anti-PRMT3 Antibody Titration: 0.2-1 ug/ml. Positive Control: HepG2 cell lysate; PRMT3 antibody - middle region (ARP32141_P050) in Human HepG2 cells using Western Blot
H00010196-A01 - PRMT3
HRMT1L3 polyclonal antibody (A01), Lot # 061025JCS1 Western Blot analysis of HRMT1L3 expression in 293 ( Cat # L026V1 ).
PAB2171 - PRMT3
Formalin-fixed and paraffin-embedded human prostate carcinoma tissue reacted with PRMT3 polyclonal antibody ( Cat # PAB2171 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
PAB2171 - PRMT3
Western blot analysis of PRMT3 polyclonal antibody ( Cat # PAB2171 ) in whole HL-60 cell lysate : PRMT3 ( Arrow ) was detected using purified polyclonal antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
PAB2172 - PRMT3
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma.
PAB2172 - PRMT3
Western blot analysis of PRMT3 polyclonal antibody ( Cat # PAB2172 ) in whole HL-60 cell lysate : PRMT3 ( Arrow ) was detected using purified polyclonal antibody ( Lane B ) but not pre-immune serum ( lane A ) . Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
AP11007PU-N - PRMT3
Figure 2. Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with PRMT3 antibody (N-term) (Cat. #AP11007PU-N), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. Clinical relevance has not been evaluated.
AP11007PU-N - PRMT3
Figure 1. Western blot analysis of anti-PRMT3 Pab (Cat. #AP11007PU-N)in whole HL60 cell lysate: PRMT3 (Arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
AP11008PU-N - PRMT3
Figure 3. Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with PRMT3 Antibody (C-term) (Cat.#AP11008PU-N), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. Clinical relevance has not been evaluated.
AP11008PU-N - PRMT3
Figure 2. Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. Clinical relevance has not been evaluated.
AP11008PU-N - PRMT3
Figure 1. Western blot analysis of anti-PRMT3 Pab (Cat. #AP11008PU-N)in whole HL60 cell lysate: PRMT3 (Arrow) was detected using purified Pab (Lane B). Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
H00010196-M16 - PRMT3
PRMT3 monoclonal antibody (M16), clone 3G4. Western Blot analysis of PRMT3 expression in HepG2(Cat # L019V1 ).
ARP40183_P050 - PRMT3
Human Muscle; WB Suggested Anti-PRMT3 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Human Muscle; PRMT3 antibody - middle region (ARP40183_P050) in Human Muscle cells using Western Blot
ARP40183_P050 - PRMT3
HCT116; Quiescent human colon carcinoma HCT116 cultures were treated with 10% FBS for three time points (0, 15, 30min) or (0, 30, 60min) were used in Matrix-ChIP and real-time PCR assays at EGR1 gene (Exon1) and 15kb upstream site.; PRMT3 antibody - middle region (ARP40183_P050) in HCT116 using CHIP
AR51778PU-N - PRMT3
TP309382 - PRMT3
TA311343 - PRMT3
(1ug/ml) staining of MCF7 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
TA322557 - PRMT3
Predicted cell location: Cytoplasm,Nucleus. Positive control: Human colon and cervical cancer tissue. Recommended dilution: 1/50-200 The image on the left is immunohistochemistry of paraffin-embedded human colon cancer tissue using PRMT3 antibody at dilution 1/30, on the right is treated with the synthetic peptide. (Original magnification:x 200)
TA322557 - PRMT3
Predicted cell location: Cytoplasm,Nucleus. Positive control: Human colon and cervical cancer tissue. Recommended dilution: 1/50-200 The image on the left is immunohistochemistry of paraffin-embedded human cervical cancer tissue using PRMT3 antibody at dilution 1/30, on the right is treated with the synthetic peptide. (Original magnification:x200)
TA329912 - PRMT3
WB Suggested Anti-PRMT3 Antibody Titration: 0.2-1 ug/ml; Positive Control: HepG2 cell lysate
TA345674 - PRMT3
WB Suggested Anti-PRMT3 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 312500; Positive Control: Human Muscle
TA345674 - PRMT3
Quiescent human colon carcinoma HCT116 cultures were treated with 10% FBS for three time points (0, 15, 30min) or (0, 30, 60min) were used in Matrix-ChIP and real-time PCR assays at EGR1 gene (Exon1) and 15kb upstream site.

12 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

PRMT3 (468-421) antibody
  Goat Anti-PRMT3 (aa468-421) Antibody  
(1ug/ml) staining of MCF7 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Hu E, WB
0.1 mg / €360.00
  OriGene Technologies, Inc.

PRMT3 antibody
  Anti-PRMT3 Rabbit Polyclonal Antibody  
Predicted cell location: Cytoplasm,Nucleus. Positive control: Human colon and cervical cancer tissue. Recommended dilution: 1/50-200 The image on the left is immunohistochemistry of paraffin-embedded human colon cancer tissue using PRMT3 antibody at dilution 1/30, on the right is treated with the synthetic peptide. (Original magnification:x 200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €335.00
  OriGene Technologies, Inc.
+1 additional image

PRMT3 (N-term) antibody
Figure 2. Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with PRMT3 antibody (N-term) (Cat. #AP11007PU-N), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. Clinical relevance has not been evaluated. Rabbit Ig Purified Hu P, WB
0.4 ml / €415.00
  OriGene Technologies GmbH
+1 additional image

PRMT3 (C-term) antibody
Figure 3. Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with PRMT3 Antibody (C-term) (Cat.#AP11008PU-N), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. Clinical relevance has not been evaluated. Rabbit Ig Purified Hu P, WB
0.4 ml / €415.00
  OriGene Technologies GmbH
+2 additional images

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

PRMT3 (transcript variant 1)
  Recombinant protein of human protein arginine methyltransferase 3 (PRMT3), transcript variant 1  
PRMT3 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.		 HEK293 cells
20 µg / €748.00
  OriGene Technologies, Inc.
 

Lysates

Catalog No.    

PRMT3 overexpression lysate
PRMT3 overexpression lysate  
0.1 mg / €295.00
  OriGene Technologies, Inc.

Mouse Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Prmt3 (GFP-tagged) - Mouse protein arginine N-methyltransferase 3 (cDNA clone MGC:58346 IMAGE:6534023), 200 uL, >10^7 TU/mL
Lenti ORF particles, Prmt3 (GFP-tagged) - Mouse protein arginine N-methyltransferase 3 (cDNA clone MGC:58346 IMAGE:6534023), 200 uL, >10^7 TU/mL  
200 µl / €1,240.00
  OriGene Technologies, Inc.
 

Lenti ORF particles, Prmt3 (GFP-tagged) - Mouse protein arginine N-methyltransferase 3 (Prmt3), 200 uL, >10^7 TU/mL
Lenti ORF particles, Prmt3 (GFP-tagged) - Mouse protein arginine N-methyltransferase 3 (Prmt3), 200 uL, >10^7 TU/mL  
200 µl / €1,220.00
  OriGene Technologies, Inc.
 

Lenti ORF particles, Prmt3 (Myc-DDK-tagged) - Mouse protein arginine N-methyltransferase 3 (cDNA clone MGC:58346 IMAGE:6534023), 200 uL, >10^7 TU/mL
Lenti ORF particles, Prmt3 (Myc-DDK-tagged) - Mouse protein arginine N-methyltransferase 3 (cDNA clone MGC:58346 IMAGE:6534023), 200 uL, >10^7 TU/mL  
200 µl / €1,110.00
  OriGene Technologies, Inc.
 

Lenti ORF particles, Prmt3 (Myc-DDK-tagged) - Mouse protein arginine N-methyltransferase 3 (Prmt3), 200 uL, >10^7 TU/mL
Lenti ORF particles, Prmt3 (Myc-DDK-tagged) - Mouse protein arginine N-methyltransferase 3 (Prmt3), 200 uL, >10^7 TU/mL  
200 µl / €1,090.00
  OriGene Technologies, Inc.
 

Rat Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Prmt3 (GFP-tagged ORF) - Rat protein arginine methyltransferase 3 (Prmt3), 200 uL, >10^7 TU/mL
Lenti ORF particles, Prmt3 (GFP-tagged ORF) - Rat protein arginine methyltransferase 3 (Prmt3), 200 uL, >10^7 TU/mL  
200 µl / €1,230.00
  OriGene Technologies, Inc.
 

Lenti ORF particles, Prmt3 (Myc-DDK-tagged ORF) - Rat protein arginine methyltransferase 3 (Prmt3), 200 uL, >10^7 TU/mL
Lenti ORF particles, Prmt3 (Myc-DDK-tagged ORF) - Rat protein arginine methyltransferase 3 (Prmt3), 200 uL, >10^7 TU/mL  
200 µl / €1,100.00
  OriGene Technologies, Inc.
 

12 Item(s)

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12 products - FILTER YOUR RESULTS!

Available Filters

Product Type
  1. Lysate (1)
  2. Mouse Lenti ORF Particle (4)
  3. Primary Antibody (4)
  4. Protein | Growth Factor (1)
  5. Rat Lenti ORF Particle (2)
Clonality
  1. Polyclonal (4)
Target
  1. PRMT3 (5)
Property
  1. 468-421 (1)
  2. C-term (1)
  3. N-term (1)
Reactivity
  1. Human (Hu) (4)
  2. Mouse (Ms) (1)
  3. Rat (Rt) (1)
Applications
  1. Paraffin Sections (P) (3)
  2. Western blot / Immunoblot (WB) (3)
  3. Enzyme Immunoassay (E) (2)
Presentation
  1. Purified (3)
  2. Aff - Purified (1)
Host
  1. Rabbit (3)
  2. Goat (1)
Images
  1. 1 image (5)
  2. 3+ images (1)
Images: Applications
  1. Western blot / Immunoblot (WB) (3)
  2. Paraffin Sections (P) (2)
  3. IHC (1)
Species (Protein)
  1. Human (1)
Source
  1. HEK293 cells (1)
Manufacturer
  1. OriGene Technologies (12)
Service
  • OriGene Technologies GmbH
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  • D-32052 Herford
  • Germany
  • phone: +49-5221-34606-0
  • free call: 0800-227 47 46 (Germany only)
  • fax: +49-5221-34606-11
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  • orders: ordering-de@origene.com
 
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