Prohibitin-2 antibody
Principal name
Prohibitin-2 antibody
Alternative names for Prohibitin-2 antibody
PHB2, BAP, REA, BAP37, D-prohibitin, B-cell receptor-associated protein BAP37, Repressor of estrogen receptor activity
Ncbi ID
9606, NP_009204, NP_001138303.1, NP_001138303, AAH14766.1, NM_001144831, NP_031557.2, NM_007531, NM_001013035
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Frozen Sections (C), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP)
Background of Prohibitin-2 antibody
- Kit Component:
- - KN313190G1, Phb2 gRNA vector 1 in pCas-Guide vector
- KN313190G2, Phb2 gRNA vector 2 in pCas-Guide vector
- KN313190D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Kategaya,L.S., et al. (2012) PLoS ONE 7 (2), E31987.
Emerson,V., et al. (2010) J. Virol. 84 (3), 1355-1365.
Formalin-fixed and paraffin-embedded human colon carcinoma tissue reacted with PHB2 polyclonal antibody ( Cat # PAB2840 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Western blot analysis of PHB2 ( arrow ) using rabbit PHB2 polyclonal antibody ( Cat # PAB2840 ) . 293 cell lysates ( 2 µg/lane ) either nontransfected ( Lane 1 ) or transiently transfected with the PHB2 gene ( Lane 2 ) ( Origene Technologies ) .
Human HepG2; WB Suggested Anti-PHB2 Antibody Titration: 0.2-1 ug/ml. Positive Control: HepG2 cell lysate. PHB2 is supported by BioGPS gene expression data to be expressed in HepG2.; PHB2 antibody - C-terminal region (ARP48316_P050) in Human HepG2 cells using Western Blot
Formalin-fixed and paraffin-embedded human colon carcinoma tissue reacted with PHB2 Antibody (AP13987PU-N), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Western blot analysis of PHB2 (arrow) using rabbit polyclonal PHB2 Antibody (AP13987PU-N). 293 cell lysates (2 µg/lane) either nontransfected (Lane 1) or transiently transfected with the PHB2 gene (Lane 2) (Origene Technologies).
Western blot analysis of PHB2 (arrow) using PHB2 Antibody (AP13988PU-N). 293 cell lysates (2 µg/lane) either nontransfected (Lane 1) or transiently transfected with the PHB2 gene (Lane 2) (Origene Technologies).
MCF-7 whole cell extract was resolved by electrophoresis, transferred to nitrocellulose and probed with rabbit anti-BAP37 antibody. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system.
Immunofluorescent microscope analysis of Hela cells using anti-BAP37 polyclonal antibody (red). Nuclei were stain with DAPI (blue).
Breast: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human MCF-7; WB Suggested Anti-PHB2 Antibody Titration: 0.2-1 ug/ml. Positive Control: MCF7 cell lysate; PHB2 antibody - N-terminal region (ARP48315_P050) in Human MCF-7 cells using Western Blot
Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Adrenal: Formalin-Fixed, Paraffin-Embedded (FFPE)
Western blot analysis of PHB2 (arrow) using rabbit polyclonal PHB2 Antibody (Human C-term) (Cat.#TA300151).
Formalin-fixed and paraffin-embedded human colon carcinoma tissue reacted with PHB2 Antibody (N-term) (Cat.#TA300151), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Sample (50 ug of whole cell lysate). A: Mouse brain. 12% SDS PAGE. TA308685 diluted at 1:1000.
Sample (30 ug of whole cell lysate). A: H1299. B: Hela. 10% SDS PAGE. TA308685 diluted at 1:1000.
Prohibitin 2 antibody detects PHB2 protein at cytosol on U87 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded U87 xenograft. Prohibitin 2 antibody (TA308685) dilution: 1:500.
Immunofluorescence analysis of paraformaldehyde-fixed A431, using REA (TA308685) antibody at 1:200 dilution.
WB Suggested Anti-PHB2 Antibody Titration: 0.2-1 ug/ml; Positive Control: HepG2 cell lysate.PHB2 is supported by BioGPS gene expression data to be expressed in HepG2
PHB2 antibody in Human Heart using Western blot
WB Suggested Anti-PHB2 Antibody Titration: 0.2-1 ug/ml; Positive Control: MCF7 cell lysate
Lysates
| Catalog No. | ||
|---|---|---|
| LY428526 | PHB2 overexpression lysate |
|
 |
0.1 mg /
€315.00
|
|
| OriGene Technologies, Inc. |