PROSC antibody
Principal name
PROSC antibody
Alternative names for PROSC antibody
Proline synthase co-transcribed bacterial homolog protein
Ncbi ID
9606, NP_009129, NP_009129.1, NM_007198, NM_001039078, NM_001039077, NM_054057, NP_473398.1
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), ELISA (detection) (E(detection)), Western blot / Immunoblot (WB), Flow Cytometry (F), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF)
Background of PROSC antibody
- Kit Component:
- - KN313958G1, Prosc gRNA vector 1 in pCas-Guide vector
- KN313958G2, Prosc gRNA vector 2 in pCas-Guide vector
- KN313958D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Ikegawa S., et al. (1999) J. Hum. Genet. 44:337-342Burkard T.R., et al. (2011) BMC Syst. Biol. 5:17-17
PROSC MaxPab polyclonal antibody. Western Blot analysis of PROSC expression in human liver.
Western Blot analysis of PROSC expression in transfected 293T cell line (H00011212-T01) by PROSC MaxPab polyclonal antibody.Lane 1: PROSC transfected lysate(30.25 KDa).Lane 2: Non-transfected lysate.
PROSC MaxPab polyclonal antibody. Western Blot analysis of PROSC expression in human liver.
Western Blot analysis of PROSC expression in transfected 293T cell line (H00011212-T01) by PROSC MaxPab polyclonal antibody.Lane 1: PROSC transfected lysate(30.25 KDa).Lane 2: Non-transfected lysate.
PROSC Antibody (C-term) (Cat. #AP53452PU-N) western blot analysis in HepG2 cell line lysates (35µg/lane).This demonstrates the PROSC antibody detected the PROSC protein (arrow).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PROSC (RC200853, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PROSC.
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-PROSC mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA505159)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-PROSC mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA505159)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-PROSC mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA505159)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-PROSC mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA505159)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-PROSC mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA505159)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-PROSC mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA505159)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PROSC (RC200853, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PROSC.
HEK293T cells transfected with either RC200853 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-PROSC antibody(TA505159), and then analyzed by flow cytometry.
Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-PROSC mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505162)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-PROSC mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505162)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-PROSC mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505162)
Anti-PROSC mouse monoclonal antibody (TA505162) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PROSC(RC200853).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PROSC (RC200853, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PROSC.
Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-PROSC monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
HEK293T cells transfected with either RC200853 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-PROSC antibody(TA505162), and then analyzed by flow cytometry.
Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-PROSC mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA505163)
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-PROSC mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA505163)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-PROSC mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA505163)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-PROSC mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA505163)
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-PROSC mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA505163)
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-PROSC mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA505163)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PROSC (RC200853, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PROSC.
HEK293T cells transfected with either RC200853 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-PROSC antibody(TA505163), and then analyzed by flow cytometry.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PROSC (RC200853, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PROSC.
HEK293T cells transfected with either RC200853 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-PROSC antibody(TA505194), and then analyzed by flow cytometry.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PROSC (RC200853, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PROSC.
Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-PROSC Rabbit Polyclonal antibody. (TA590555)
Immunohistochemistry: PROSC Antibody - A majority of the normal cells displayed moderate to strong cytoplasmic positivity. Most of the malignant cells showed moderate cytoplasmic immunoreactivity. A few cases of ovarian cancers were strongly stained while most cases of melanomas and lymphomas were weakly positive. Image and statement courtesy of the Human Protein Atlas (HPA).
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-PROSC Rabbit Polyclonal antibody. (TA590555)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-PROSC Rabbit Polyclonal antibody. (TA590555)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue using anti-PROSC Rabbit Polyclonal antibody. (TA590555)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PROSC (RC200853, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PROSC.
Western Blot: PROSC Antibody - Samples: Lane 1, Marker: 250, 130, 95, 72, 55, 36, 28, 17, 11 Lane 2, RT-4 Lane 3, U-251MG sp Lane 4, Human Plasma Lane 5, Liver Lane 6, Tonsil , Target weight: 30 Validation score: 2 Validation description: Supportive - Band of predicted size in kDa (+/-20%) with additional bands present.
ELISA: PROSC Antibody
- Quick-Filters:
- Highly Validated (4)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA505159 | PROSC antibody |
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| PROSC mouse monoclonal antibody, clone OTI3G7 (formerly 3G7) | |||||||
 |
Mouse | IgG1 | OTI3G7 | Purified | Hu | F, P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
 +7 additional images |
Highly validated antibody!
|
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| TA505162 | PROSC antibody |
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| PROSC mouse monoclonal antibody, clone OTI1G2 (formerly 1G2) | |||||||
 |
Mouse | IgG1 | OTI1G2 | Purified | Hu | F, ICC/IF, P, WB |
0.1 ml /
€379.00
|
| OriGene Technologies, Inc. | |||||||
| +6 additional images |
Highly validated antibody!
|
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| TA505163 | PROSC antibody |
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| PROSC mouse monoclonal antibody, clone OTI3A6 (formerly 3A6) | |||||||
 |
Mouse | IgG1 | OTI3A6 | Purified | Hu | F, P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +7 additional images |
Highly validated antibody!
|
||||||
| TA590555 | PROSC antibody |
||||||
| Rabbit Polyclonal PROSC Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu | E, P, WB |
0.1 mg /
€409.00
|
|
| OriGene Technologies, Inc. | |||||||
| +7 additional images |
Highly validated antibody! |
||||||
| TA505130 | PROSC antibody |
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| PROSC mouse monoclonal antibody, clone OTI3H4 (formerly 3H4) | |||||||
 |
Mouse | IgG1 | OTI3H4 | Purified | Hu | WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
|
|||||||
| TA505194 | PROSC antibody |
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| PROSC mouse monoclonal antibody, clone OTI2H1 (formerly 2H1) | |||||||
 |
Mouse | IgG1 | OTI2H1 | Purified | Hu | F, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|
||||||
| TA505195 | PROSC antibody |
||||||
| PROSC mouse monoclonal antibody, clone OTI2C2 (formerly 2C2) | |||||||
 |
Mouse | IgG1 | OTI2C2 | Purified | Hu | WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
|
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| AP53452PU-N | PROSC (C-term) antibody |
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 |
Rabbit | Ig | Aff - Purified | Hu | WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies GmbH | |||||||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP300853 | PROSC |
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| Recombinant protein of human proline synthetase co-transcribed homolog (bacterial) (PROSC) | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY416132 | PROSC overexpression lysate |
|
 |
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. |
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR220705L2V | Lenti ORF particles, Prosc (GFP-tagged) - Mouse proline synthetase co-transcribed (Prosc), transcript variant 1, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€930.00
|
|
| OriGene Technologies, Inc. | ||
| MR201110L2V | Lenti ORF particles, Prosc (GFP-tagged) - Mouse proline synthetase co-transcribed (Prosc), transcript variant 2, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€930.00
|
|
| OriGene Technologies, Inc. | ||
| MR200908L2V | Lenti ORF particles, Prosc (GFP-tagged) - Mouse proline synthetase co-transcribed (Prosc), transcript variant 3, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€930.00
|
|
| OriGene Technologies, Inc. | ||
| MR201110L1V | Lenti ORF particles, Prosc (Myc-DDK-tagged) - Mouse proline synthetase co-transcribed (Prosc), transcript variant 2, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€830.00
|
|
| OriGene Technologies, Inc. | ||
| MR200908L1V | Lenti ORF particles, Prosc (Myc-DDK-tagged) - Mouse proline synthetase co-transcribed (Prosc), transcript variant 3, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€830.00
|
|
| OriGene Technologies, Inc. | ||
