PROX-1 antibody
Principal name
PROX-1 antibody
Alternative names for PROX-1 antibody
PROX1, Prospero homeobox protein 1, Homeobox prospero-like protein PROX1
Ncbi ID
NP_002754.2, 9606, NP_002754, NM_002763, NP_032963.1, NM_008937
Available reactivities
Available hosts
Available applications
Frozen Sections (C), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), Enzyme Immunoassay (E)
Background of PROX-1 antibody
The expression pattern of the Prox1 homeo box gene suggests that it has a role in a variety of embryonic tissues, including lens. Analysis of mRNA reveals that Prox mRNA is present in many different human tissues and that lens demonstrated the highest level. Homozygous Prox1-null mice die at midgestation from multiple developmental defects, and a targeted effect on lens development has been reported. Prox1 inactivation caused abnormal cellular proliferation, downregulated expression of the cell cycle inhibitors Cdkn1b and Cdkn1c, misexpression of E-cadherin, and excessive apoptosis. Consequently, mutant lens cells failed to polarize and elongate properly, resulting in a hollow lens. The Prox1 gene is expressed in a subpopulation of endothelial cells that by budding and sprouting give rise to the lymphatic system. Prox1 appears to be a specific and required regulator of the development of the lymphatic system. Prox1 also has been document to be required for hepatocyte migration in the mouse. Loss of Prox1 results in a smaller liver with a reduced population of clustered hepatocytes. The homeodomain protein Prox1 regulates the egress of progenitor cells from the cell cycle in the embryonic mouse retina. Cells lacking Prox1 are less likely to stop dividing, and ectopic expression of Prox1 forces progenitor cells to exit the cell cycle. Prox1 acts as a key participant in progenitor-cell proliferation and cell-fate determination in the vertebrate retina.
General readings
1. Joshua D. Webster, Margaret A. Miller, Dee DuSold, and José Ramos-Vara. Effects of Prolonged Formalin-fixation on Diagnostic Immunohistochemistry in Domestic Animals.
J. Histochem. Cytochem., Apr 2009; 10.1369/jhc.2009.953877.
2. Ohad Shaham, April N. Smith, Michael L. Robinson, Makoto M. Taketo, Richard A. Lang, and Ruth Ashery-Padan. Pax6 is essential for lens fiber cell differentiation. Development, Aug 2009; 136: 2567-2578.
DP3501 PROX-1 Antibody Staining of ParaffinEmbedded Human Mucosa Sections.
DP3501 PROX-1 Antibody Immunostaining of Human Lymphatic Endothelial Cells.
Immunofluorescence with a Frozen Section of a Mouse embryo at day 13.5 with anti-CD31 Antibody [Green] and anti-Human Prox-1 Antibody [red] (Cat.-No DP3516). It shows a large lymphatic vessel (lv), blood vessels (arrows) and a sympathetic ganglion (sg) which is also positiv for Prox-1. The experiment was performed by PD. Dr. Jörg Wilting, Pädiatrie I, Zentrum für Kinderheilkunde und Jugendmedizin in Göttingen, Germany.
Western analysis with anti-human Prox-1 antibody Cat.-No DP3516 (Left) and DP3516P (Right).
Cross reaction of anti-Human Prox-1 with Horse tissue sections: Equine mandibular lymph node,paraffin section, stained for Prox1 antibody (Cat#DP3516P) counterstained with toluidine blue. Sinusoids (S) as well as lymphatic vessels in the hilum of the lymph node (arrows) are lined by positively stained lymphatic endothelial cells. Blood vascular endothelium remains unstained (arrow heads). LC: Cluster of lymphocytes. From: Staszyk,C., K. F. Duesterdieck, H. Gasseu. A. Bienert, Immunohistochemical identification of lymphatic vessels in the periodontium of equine cheek teeth. J. Vet. Dent. 22,227–232,2005
Prox1 protein in E15 chick pancreas tissue detected by immunofluorescence studies (IF) using a polyclonal rabbit anti-human Prox1 antibody (Cat.-No DP3516P). [diluted: 1:200]
Both experiments were performed by Andreia Margarido (de Santa Barbara’s team); INSERM U1046, Montpellier, France
Both experiments were performed by Andreia Margarido (de Santa Barbara’s team); INSERM U1046, Montpellier, France
Immunofluorescence with a Frozen Sections of a Mouse embryo at day 13.5 with anti-CD31 [green] and anti-Human Prox-1 [red] (Cat.-No DP3516S). It shows a large lymphatic vessel (lv), blood vessels (arrows) and a sympathetic ganglion (sg) which is also positiv for Prox-1 (Fig.1). The experiment was performed by PD. Dr. Jörg Wilting, Pädiatrie I, Zentrum für Kinderheilkunde und Jugendmedizin in Göttingen, Germany.
Immunofluorescence staining (green) of cryo-sections of human foreskin (fixed 15 min in 4% PFA) with anti-human Prox-1 (dilution 1:200) (Cat.-No DP3516P) and counter staining of nuclei with Dapi. A) Note the specific green Prox-1 signal in the nuclei of lymphatics (L). Ep, epidermis. Specimen provided by Prof. Dr. J. Wilting, Goettingen. The experiment was performed by PD. Dr. Jörg Wilting, Pädiatrie I, Zentrum für Kinderheilkunde und Jugendmedizin in Göttingen, Germany.
Immunofluorescence staining (green) of primary human dermal lymphatic endothelial cells (HDLEC) with anti-human Prox-1 (1μg/ml) Cat.-No DP3516P and counter staining of nuclei with Dapi. As secondary antibody goat anti-rabbit ALEXA Flour 488 was used 1:400.
Western analysis with anti-human Prox-1 antibody Cat.-No DP3516 (Left) and DP3516P (Right).
Prox1 protein in E15 chick pancreas tissue detected by immunohistochemistry studies (IHC) using a polyclonal Rabbit anti-Human Prox1 antibody Cat.-No DP3516P at 1/400.
Figure 1. Immunofluorescence with a Frozen Section of a Mouse embryo at day 13.5 with anti-CD31 [green] and anti-Human Prox-1 [red] (Cat.-No DP3516S). It shows a large lymphatic vessel (lv), blood vessels (arrows) and a sympathetic ganglion (sg) which is also positiv for Prox-1 (Fig.1). The experiment was performed by PD. Dr. Jörg Wilting, Pädiatrie I, Zentrum für Kinderheilkunde und Jugendmedizin in Göttingen, Germany.
Detection limit for recombinant GST tagged PROX1 is approximately 1ng/ml as a capture antibody.
Detection limit for recombinant GST tagged PROX1 is approximately 3ng/ml as a capture antibody.
PROX1 monoclonal antibody (M09), clone 1H6. Western Blot analysis of PROX1 expression in human skeletal muscle.
PROX1 monoclonal antibody (M09), clone 1H6 Western Blot analysis of PROX1 expression in MCF-7 ( Cat # L046V1 ).
PROX1 monoclonal antibody (M09), clone 1H6. Western Blot analysis of PROX1 expression in SW-13(Cat # L005V1 ).
Detection limit for recombinant GST tagged PROX1 is approximately 3ng/ml as a capture antibody.
The PROX1 polyclonal antibody ( Cat # PAB1918 ) is used in Western blot to detect PROX1 in mouse brain tissue lysate.
Formalin-fixed and paraffin-embedded human hepatocellular cancer tissue reacted with PROX1 polyclonal antibody ( Cat # PAB1919 ) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Formalin-fixed and paraffin-embedded human hepatocellular cancer tissue reacted with PROX1 polyclonal antibody ( Cat # PAB1920 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Western blot analysis of PROX1 ( arrow ) using PROX1 polyclonal antibody ( Cat # PAB1920 ) . 293 cell lysates ( 2 µg/lane ) either nontransfected ( Lane 1 ) or transiently transfected with the PROX1 gene ( Lane 2 ) ( Origene Technologies ) .
Western blot analysis of PROX1 polyclonal antibody ( Cat # PAB1920 ) in HepG2 cell line lysates ( 35 µg/lane ) . PROX1 ( arrow ) was detected using the purified polyclonal antibody ( 1 : 60 dilution ) .
The anti-PROX1 N-term (AP11899PU-N) is used in Western blot to detect PROX1 in mouse brain tissue lysate.
Western blot analysis of extracts from A431 cells using Prospero homeobox protein 1 Antibody AP26049PU-N and the same antibody preincubated with blocking peptide.
SDS-PAGE analysis of recombinant Human Prox-1 Sample was loaded in 15% SDS-polyacrylamide gel under reducing conditions and stained with Coomassie blue.
Western blot analysis of anti-PROX1 Antibody (Center) (Cat.#TA302031) in HepG2 cell line lysates (35ug/lane). PROX1(arrow) was detected using the purified Pab.
Western blot analysis of PROX1 (arrow) using rabbit polyclonal PROX1 Antibody (Center) (Cat.#TA302031). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PROX1 gene (Lane 2) (Origene Technologies).
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with PROX1 antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Immunohistochemical staining of rat dentate gyrus showing specific immunolabeling of the prox1 protein. Photo courtesy of Justin Kievits and Teresa Milner, Weill Cornell Medical College.
Western blot analysis of PROX1 (arrow) using rabbit polyclonal PROX1 Antibody
PROX1 Antibody (K507) (Cat. #TA324702) western blot analysis in A2058 cell line lysates (35ug/lane).This demonstrates the PROX1 antibody detected the PROX1 protein (arrow).
IF image of A2058 cell stained with PROX1 Antibody (C-term)(Cat#TA324702).A2058 cells were incubated with PROX1 primary antibody (1:25, 1 h at 37?). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7 units/ml). Nuclei were counterstained with DAPI (blue) . PROX1 immunoreactivity is localized to Nucleus significantly.
Gel: 6%SDS-PAGE,Lysate: 40 ug,Lane 1-2: Human fetal liver tissue, 231 cells,Primary antibody: (PROX1 Antibody) at dilution 1/300 dilution,Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution,Exposure time: 2 minutes
The image on the left is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using (PROX1 Antibody) at dilution 1/40, on the right is treated with synthetic peptide. (Original magnification: ×200)
- Quick-Filters:
- Cited (2)
- Highly Validated (1)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| DP3501 | PROX-1 (C-term) antibody |
||||||
 |
Rabbit | Serum | Hu, Ms, Rt, Ze | C, P, WB |
0.2 ml /
€400.00
|
||
| OriGene Technologies GmbH | |||||||
 +1 additional image |
Published recently! |
||||||
| DP3516 | PROX-1 (C-term) antibody |
||||||
![Immunofluorescence with a Frozen Section of a Mouse embryo at day 13.5 with anti-CD31 Antibody [Green] and anti-Human Prox-1 Antibody [red] (Cat.-No DP3516). It shows a large lymphatic vessel (lv), blood vessels (arrows) and a sympathetic ganglion (sg) which is also positiv for Prox-1.
The experiment was performed by PD. Dr. Jörg Wilting, Pädiatrie I, Zentrum für Kinderheilkunde und Jugendmedizin in Göttingen, Germany.](https://m1.acris-antibodies.com/catalog/product/cache/1/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/0092801.jpeg) |
Rabbit | Purified | Hu, Ms, Rt | ICC/IF, P, WB |
0.2 mg /
€350.00
|
||
| OriGene Technologies GmbH | |||||||
| +1 additional image |
Published recently! |
||||||
| DP3516P | PROX-1 (C-term) antibody |
||||||
 |
Rabbit | IgG | Aff - Purified | Hu, Ms, Rt | C, E, ICC/IF, P, WB |
50 µg /
€280.00
|
|
| OriGene Technologies GmbH | |||||||
| +6 additional images |
Highly validated antibody! |
||||||
| TA302031 | PROX-1 antibody |
||||||
| Rabbit Polyclonal Antibody against PROX1 (Center) | |||||||
 |
Rabbit | Ig | Aff - Purified | Hu, Ms | P, WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
|||||||
| TA309222 | PROX-1 antibody |
||||||
| mouse Anti-Prox1 Antibody | |||||||
 |
Mouse | IgG | 5G10 | Aff - Purified | Chk, Hu, Ms, Rt | P |
0.1 ml /
€405.00
|
| OriGene Technologies, Inc. | |||||||
| TA324702 | PROX-1 (C-term) antibody |
||||||
| Rabbit polyclonal PROX1 Antibody (C-term) | |||||||
 |
Rabbit | Ig | Purified | Hu, Ms | P, WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
|||||||
| TA351557 | PROX-1 antibody |
||||||
| Rabbit Polyclonal Anti-PROX1 Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms | E, P, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|||||||
| AP11899PU-N | PROX-1 (N-term) antibody |
||||||
 |
Rabbit | Ig | Purified | Hu, Ms | WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies GmbH | |||||||
| AP26049PU-N | PROX-1 (723-727) antibody |
||||||
 |
Rabbit | Aff - Purified | Hu, Ms | WB |
0.1 mg /
€300.00
|
||
| OriGene Technologies GmbH | |||||||
| AP26049PU-S | PROX-1 (723-727) antibody |
||||||
|
Rabbit | Aff - Purified | Hu, Ms | WB |
50 µg /
€230.00
|
||
| OriGene Technologies GmbH | |||||||
| DP3501PS | PROX-1 (C-term) antibody |
||||||
 |
Rabbit | Purified | Fi, Hu, Ms, Rt | C, P, WB |
0.1 mg /
€400.00
|
||
| OriGene Technologies GmbH | |||||||
| DP3516B | PROX-1 (C-term) antibody |
||||||
|
Rabbit | Biotin | Hu, Ms, Rt | ICC/IF |
50 µg /
€350.00
|
||
| OriGene Technologies GmbH | |||||||
| DP3516S | PROX-1 (C-term) antibody |
||||||
![Figure 1. Immunofluorescence with a Frozen Section of a Mouse embryo at day 13.5 with anti-CD31 [green] and anti-Human Prox-1 [red] (Cat.-No DP3516S). It shows a large lymphatic vessel (lv), blood vessels (arrows) and a sympathetic ganglion (sg) which is also positiv for Prox-1 (Fig.1). The experiment was performed by PD. Dr. Jörg Wilting, Pädiatrie I, Zentrum für Kinderheilkunde und Jugendmedizin in Göttingen, Germany.](https://m1.acris-antibodies.com/catalog/product/cache/1/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/0219346.jpeg) |
Rabbit | Purified | Hu, Ms, Rt | C, ICC/IF, WB |
0.1 mg /
€270.00
|
||
| OriGene Technologies GmbH | |||||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY400978 | PROX1 overexpression lysate |
|
|
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. |
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR210370L2V | Lenti ORF particles, Prox1 (GFP-tagged) - Mouse prospero-related homeobox 1 (Prox1), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,530.00
|
|
| OriGene Technologies, Inc. | ||
| MR210370L1V | Lenti ORF particles, Prox1 (Myc-DDK-tagged) - Mouse prospero-related homeobox 1 (Prox1), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,370.00
|
|
| OriGene Technologies, Inc. | ||
