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PRPF3 antibody

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Principal name

PRPF3 antibody

Alternative names for PRPF3 antibody

HPRP3, PRP3, Pre-mRNA-splicing factor 3, U4/U6 snRNP 90 kDa protein, U4/U6 small nuclear ribonucleoprotein Prp3

SwissProt ID

O43395 (Human), Q2KIA6 (Bovin), Q5ZJ85 (Chick), Q922U1 (Mouse)

Gene ID

9129 (PRPF3)

Ncbi ID

9606, NP_004689, NP_004689.1, NM_004698

Available reactivities

Hu (Human), Bov (Bovine), Can (Canine), Chk (Chicken), Ms (Mouse), Por (Porcine), Rt (Rat), African clawed frog, Hst (Hamster), Eq (Equine), GP (Guinea Pig), Rb (Rabbit)

Available hosts

Mouse, Rabbit, Rat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP)

Background of PRPF3 antibody

Retinitis pigmentosa (RP) is an inheritable disease characterized by progressive retinal degeneration and loss of rod photoreceptor cells, leading to total blindness. Recently three genes responsible for autosomal dominant RP (HPRP3, PRPF31, and PRPC8) have been identified as the human orthologues of the yeast genes PRP3, PRP31, and PRP8, respectively. The removal of introns from nuclear pre-mRNAs occurs on spliceosomes, which consist of 4 small nuclear ribonucleoprotein (snRNP) particles and a number of transiently associated splicing factors. All three PRP (pre-mRNA processing factor) genes are involved in the function of the U4/U6,U5 tri-snRNP, the spliceosome component required for the transition to a catalytically active state. In mammals, the 63 kDa HPRP3 is thought to recruit HPRP4 to the U4/U6 snRNP. In yeast, PRP3 and PRP4 genetically interact, and physical interactions between Prp3p and Prp4p proteins are required for association of Prp3p and Prp4p with U4/U6.

General readings

Rual,J.F., (2005) Nature 437 (7062), 1173-1178

H00009129-M01 - PRPF3
PRPF3 monoclonal antibody (M01), clone 3H6 Western Blot analysis of PRPF3 expression in Hela NE ( Cat # L013V3 ).
H00009129-M01 - PRPF3
Detection limit for recombinant GST tagged PRPF3 is approximately 0.03ng/ml as a capture antibody.
ARP40543_P050 - PRPF3
Human HepG2; WB Suggested Anti-PRPF3 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: HepG2 cell lysate; PRPF3 antibody - N-terminal region (ARP40543_P050) in Human HepG2 cells using Western Blot
AM26502AF-N - PRPF3
Immunocytochemical detection of Prp3 on HEp-Ⅱ cells using AM26502AF-N.
AM26502AF-N - PRPF3
Western blot analysis of Prp3 expression in Jurkat cells (1), HeLa cells (2), Raji cells (3), HL60 cells (4), A431 cells (5), L5178Y cells (6), WR19L cells (7), NIH/3T3 cells (8), CHO cells (9), PC12 cells (10) and Rat1 cells (11) using AM26502AF-N. (The clear band was detected in human cell lines at 116KDa. But the epitope is unclear and is thought that it is the crossreactivity of this monoclonal antibody.)
AM26502AF-N - PRPF3
Immunoprecipitation of Prp3 from Jurkat cells with AM26502AF-N (1) or Rat IgG 2a(2) and from L5178Y cells with AM26502AF-N (3) or Rat IgG2a (4). After immunoprecipitated with the antibody, immunocomplex was resolved on SDS-PAGE and immunoblotted with AM26502AF-N.
TA332715 - PRPF3
Western blot analysis of extracts of various cell lines, using PRPF3 antibody.
TA332715 - PRPF3
Immunofluorescence analysis of U20S cell using PRPF3 antibody. Blue: DAPI for nuclear staining.
TA345894 - PRPF3
WB Suggested Anti-PRPF3 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 62500; Positive Control: HepG2 cell lysate

3 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

PRPF3 antibody
  Rabbit anti-PRPF3 Polyclonal Antibody  
Western blot analysis of extracts of various cell lines, using PRPF3 antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €335.00
  OriGene Technologies, Inc.
+1 additional image

PRPF3 antibody
Immunocytochemical detection of Prp3 on HEp-Ⅱ cells using AM26502AF-N. Rat IgG2a 4E3 Azide Free Hst, Hu, Ms, Rt ICC/IF, IP, WB
0.1 mg / €430.00
  OriGene Technologies GmbH
+2 additional images

Lysates

Catalog No.    

PRPF3 overexpression lysate
PRPF3 overexpression lysate  
0.1 mg / €295.00
  OriGene Technologies, Inc.

3 Item(s)

per page

Available Filters

Product Type
  1. Lysate (1)
  2. Primary Antibody (2)
Clonality
  1. Monoclonal (1)
  2. Polyclonal (1)
Target
  1. PRPF3 (2)
Reactivity
  1. Human (Hu) (2)
  2. Mouse (Ms) (2)
  3. Rat (Rt) (2)
  4. Hamster (Hst) (1)
Applications
  1. Immunocytochemistry/​Immunofluorescence (ICC/IF) (1)
  2. Immunoprecipitation (IP) (1)
  3. Western blot / Immunoblot (WB) (1)
Presentation
  1. Azide Free (1)
  2. Purified (1)
Host
  1. Rabbit (1)
  2. Rat (1)
Images
  1. 1 image (2)
  2. 3+ images (1)
Images: Applications
  1. Western blot / Immunoblot (WB) (2)
  2. Immunocytochemistry/​Immunofluorescence (ICC/IF) (1)
  3. Immunofluorescence (IF) (1)
  4. Immunoprecipitation (IP) (1)
Manufacturer
  1. OriGene Technologies (3)
Service
  • OriGene Technologies GmbH
  • Schillerstraße 5
  • D-32052 Herford
  • Germany
  • phone: +49-5221-34606-0
  • free call: 0800-227 47 46 (Germany only)
  • fax: +49-5221-34606-11
  • email: info-de@origene.com
  • orders: ordering-de@origene.com
 
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