PRPF31 antibody
Principal name
PRPF31 antibody
Alternative names for PRPF31 antibody
PRP31,Pre-mRNA-processing factor 31, NY-BR-99, U4/U6 small nuclear ribonucleoprotein Prp31, Serologically defined breast cancer antigen NY-BR-99
SwissProt ID
O42904 (Schpo), P49704 (Yeast), Q5U5C5 (Xenla), Q7SXM7 (Danre), Q8CCF0 (Mouse), Q8WWY3 (Human)
Ncbi ID
9606, NP_056444, NP_056444.3, NM_027328, NM_015629, NP_001153186.1
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection))
Background of PRPF31 antibody
- Kit Component:
- - KN313968G1, Prpf31 gRNA vector 1 in pCas-Guide vector
- KN313968G2, Prpf31 gRNA vector 2 in pCas-Guide vector
- KN313968D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Yuan L, Kawada M, Havlioglu N, Tang H, Wu JY.
Mutations in PRPF31 inhibit pre-mRNA splicing of rhodopsin gene and cause apoptosis of retinal cells.
J Neurosci. 2005 Jan 19;25(3):748-57.
PMID: 15659613
PRPF31 polyclonal antibody (A01), Lot # 060619JCS1 Western Blot analysis of PRPF31 expression in HeLa ( Cat # L013V1 ).
PRPF31 polyclonal antibody ( Cat # PAB7154 ) ( 1 µg/ml ) staining of HeLa cell lysate ( 35 µg protein in RIPA buffer ) with ( B ) and without ( A ) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence.
AP20107PU-N PRPF31 Antibody staining (0.3 µg/ml) of cell line A431 lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
TA302582 (1µg/ml) staining of HeLa cell lysate (35µg protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence.
Western blot analysis of extracts of various cell lines, using PRPF31 antibody.
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| AP20107PU-N | PRPF31 (C-term) antibody |
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 |
Goat | Aff - Purified | Can, Hu | E, WB |
0.1 mg /
€380.00
|
||
| OriGene Technologies GmbH | |||||||
OriGene recommends this product for Western blotting. |
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| TA302582 | PRPF31 antibody |
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| Goat Polyclonal Antibody against PRPF31 | |||||||
 |
Goat | Aff - Purified | Can, Hu, Ms, Rt | E, WB |
0.1 mg /
€360.00
|
||
| OriGene Technologies, Inc. | |||||||
| TA332869 | PRPF31 antibody |
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| Rabbit anti-PRPF31 Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt |
0.1 mg /
€335.00
|
||
| OriGene Technologies, Inc. | |||||||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP318307 | PRPF31 |
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| Recombinant protein of human PRP31 pre-mRNA processing factor 31 homolog (S. cerevisiae) (PRPF31) | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR208002L2V | Lenti ORF particles, Prpf31 (GFP-tagged) - Mouse PRP31 pre-mRNA processing factor 31 homolog (yeast) (Prpf31), transcript variant 1, 200 uL, >10^7 TU/mL |
|
 |
200 µl /
€1,190.00
|
|
| OriGene Technologies, Inc. | ||
| MR208002L1V | Lenti ORF particles, Prpf31 (Myc-DDK-tagged) - Mouse PRP31 pre-mRNA processing factor 31 homolog (yeast) (Prpf31), transcript variant 1, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,060.00
|
|
| OriGene Technologies, Inc. | ||
