PRPSAP2 antibody
Principal name
PRPSAP2 antibody
Alternative names for PRPSAP2 antibody
PRPP synthetase-associated protein 2, PAP41, 41 kDa phosphoribosypyrophosphate synthetase-associated protein, Phosphoribosyl pyrophosphate synthetase-associated protein 2
SwissProt ID
A2VDS0 (Bovin), O08618 (Rat), O60256 (Human), Q28DH0 (Xentr), Q5RBA8 (Ponab), Q5ZL26 (Chick), Q8R574 (Mouse)
Ncbi ID
NP_002758, NP_002758.1, 9606, NP_001157714.1, NM_001164244, NM_001164243, NM_001164242, NM_002767, NM_057131, NM_144806
Available reactivities
Available hosts
Available applications
Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Flow Cytometry (F), Paraffin Sections (P)
Background of PRPSAP2 antibody
- Kit Component:
- - KN210719G1, PRPSAP2 gRNA vector 1 in pCas-Guide vector
- KN210719G2, PRPSAP2 gRNA vector 2 in pCas-Guide vector
- KN210719D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Katashima,R., et al. (1998) Biochim. Biophys. Acta 1396 (3), 245-250
Human Hela; Host:Rabbit. Target Name:PRPSAP2. Sample Tissue:Hela. Antibody Dilution: 1.0ug/ml. PRPSAP2 is supported by BioGPS gene expression data to be expressed in HeLa.; PRPSAP2 Antibody - N-terminal region (ARP56445_P050) in Human Hela cells using Western Blot
Human HeLa; Host: Rabbit. Target Name: PRPSAP2. Sample Tissue: Hela Whole Cell lysates. Antibody Dilution: 2.0ug/ml. PRPSAP2 is supported by BioGPS gene expression data to be expressed in HeLa.; PRPSAP2 Antibody - N-terminal region (ARP56445_P050) in Human HeLa cells using Western Blot
Human 721_B; Host:Rabbit. Target Name:PRPSAP2. Sample Tissue:721_B. Antibody Dilution: 1.0ug/ml. PRPSAP2 is supported by BioGPS gene expression data to be expressed in 721_B.; PRPSAP2 Antibody - N-terminal region (ARP56445_P050) in Human 721_B cells using Western Blot
Human Jurkat; Host:Rabbit. Target Name:PRPSAP2. Sample Tissue:Jurkat. Antibody Dilution: 1.0ug/ml. PRPSAP2 is supported by BioGPS gene expression data to be expressed in Jurkat.; PRPSAP2 Antibody - N-terminal region (ARP56445_P050) in Human Jurkat cells using Western Blot
Human HepG2; WB Suggested Anti-PRPSAP2 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:100. Positive Control: HepG2 cell lysate; PRPSAP2 Antibody - N-terminal region (ARP56445_P050) in Human HepG2 cells using Western Blot
Human MCF7; Host:Rabbit. Target Name:PRPSAP2. Sample Tissue:MCF7. Antibody Dilution: 1.0ug/ml. PRPSAP2 is supported by BioGPS gene expression data to be expressed in MCF7.; PRPSAP2 Antibody - N-terminal region (ARP56445_P050) in Human MCF7 cells using Western Blot
Hum. Fetal Brain; Host:Rabbit. Target Name:PRPSAP2. Sample Tissue:Human Fetal Brain. Antibody Dilution: 1.0ug/ml.; PRPSAP2 Antibody - N-terminal region (ARP56445_P050) in Hum. Fetal Brain cells using Western Blot
Human Heart; WB Suggested Anti-PRPSAP2 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:12500. Positive Control: Human heart; PRPSAP2 antibody - middle region (ARP56446_P050) in Human Heart cells using Western Blot
Immunofluorescence of purified MaxPab antibody to PRPSAP2 on HeLa cell. [antibody concentration 10 ug/ml]
Western Blot analysis of PRPSAP2 expression in transfected 293T cell line (H00005636-T01) by PRPSAP2 MaxPab polyclonal antibody.Lane 1: PRPSAP2 transfected lysate(40.59 KDa).Lane 2: Non-transfected lysate.
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-PRPSAP2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501563)
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-PRPSAP2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501563)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-PRPSAP2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501563)
Anti-PRPSAP2 mouse monoclonal antibody (TA501563) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PRPSAP2(RC210719).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PRPSAP2 (RC210719, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PRPSAP2.
HEK293T cells transfected with either RC210719 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-PRPSAP2 antibody(TA501563), and then analyzed by flow cytometry.
Sample (50 ug of whole cell lysate). A: mouse liver. 12% SDS PAGE. TA308166 diluted at 1:1000.
Sample (30ug whole cell lysate). A:HeLa S3. 7.5% SDS PAGE. TA308166 diluted at 1:1000
Immunohistochemical analysis of paraffin-embedded A549 xenograft, using PRPSAP2(TA308166) antibody at 1:500 dilution.
Immunofluorescence analysis of methanol-fixed HeLa, using PRPSAP2(TA308166) antibody at 1:500 dilution.
WB Suggested Anti-PRPSAP2 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:100; Positive Control: HepG2 cell lysate
Host:Rabbit; Target Name:PRPSAP2; Sample Tissue:Human Fetal Brain; Antibody Dilution: 1.0 ug/ml
Host:Rabbit; Target Name:PRPSAP2; Sample Tissue:Jurkat; Antibody Dilution: 1.0 ug/mlPRPSAP2 is supported by BioGPS gene expression data to be expressed in Jurkat
Host:Rabbit; Target Name:PRPSAP2; Sample Tissue:MCF7; Antibody Dilution: 1.0 ug/mlPRPSAP2 is supported by BioGPS gene expression data to be expressed in MCF7
Host:Rabbit; Target Name:PRPSAP2; Sample Tissue:721_B; Antibody Dilution: 1.0 ug/mlPRPSAP2 is supported by BioGPS gene expression data to be expressed in 721_B
Host:Rabbit; Target Name:PRPSAP2; Sample Tissue:Hela; Antibody Dilution: 1.0 ug/mlPRPSAP2 is supported by BioGPS gene expression data to be expressed in HeLa
Host: Rabbit; Target Name: PRPSAP2; Sample Tissue: Hela Whole Cell lysates; Antibody Dilution: 2.0 ug/mlPRPSAP2 is supported by BioGPS gene expression data to be expressed in HeLa
WB Suggested Anti-PRPSAP2 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:12500; Positive Control: Human heart
- Quick-Filters:
- Highly Validated (2)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA308166 | PRPSAP2 antibody |
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| Rabbit Polyclonal antibody to PRPSAP2 (phosphoribosyl pyrophosphate synthetase-associated protein 2) | |||||||
 |
Rabbit | IgG | Aff - Purified | Hu, Ms | ICC/IF, P, WB |
0.1 ml /
€415.00
|
|
| OriGene Technologies, Inc. | |||||||
 +3 additional images |
Highly validated antibody! |
||||||
| TA501563 | PRPSAP2 antibody |
||||||
| PRPSAP2 mouse monoclonal antibody, clone OTI1E3 (formerly 1E3) | |||||||
 |
Mouse | IgG1 | OTI1E3 | Purified | Hu | F, ICC/IF, P, WB |
0.1 ml /
€379.00
|
| OriGene Technologies, Inc. | |||||||
| +5 additional images |
Highly validated antibody!
|
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Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP310719 | PRPSAP2 |
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| Recombinant protein of human phosphoribosyl pyrophosphate synthetase-associated protein 2 (PRPSAP2) | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR205678L2V | Lenti ORF particles, Prpsap2 (GFP-tagged) - Mouse phosphoribosyl pyrophosphate synthetase-associated protein 2 (Prpsap2), transcript variant 1, 200 uL, >10^7 TU/mL |
|
 |
200 µl /
€930.00
|
|
| OriGene Technologies, Inc. | ||
| MR205678L1V | Lenti ORF particles, Prpsap2 (Myc-DDK-tagged) - Mouse phosphoribosyl pyrophosphate synthetase-associated protein 2 (Prpsap2), transcript variant 1, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€830.00
|
|
| OriGene Technologies, Inc. | ||
Rat Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RR211579L2V | Lenti ORF particles, Prpsap2 (GFP-tagged ORF) - Rat phosphoribosyl pyrophosphate synthetase-associated protein 2 (Prpsap2), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€930.00
|
|
| OriGene Technologies, Inc. | ||
| RR211579L1V | Lenti ORF particles, Prpsap2 (Myc-DDK-tagged ORF) - Rat phosphoribosyl pyrophosphate synthetase-associated protein 2 (Prpsap2), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€830.00
|
|
| OriGene Technologies, Inc. | ||