PRUNE antibody
Principal name
PRUNE antibody
Alternative names for PRUNE antibody
Protein prune homolog, hPrune, HTcD37, DRES17, DRES-17, Drosophila-related expressed sequence 17
Ncbi ID
9606, NP_067045, NP_067045.1, NM_021222, NM_001007697, NP_775482.1
Available reactivities
Available hosts
Available applications
ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Flow Cytometry (F), Paraffin Sections (P), Enzyme Immunoassay (E)
Background of PRUNE antibody
- Kit Component:
- - KN314060G1, Prune gRNA vector 1 in pCas-Guide vector
- KN314060G2, Prune gRNA vector 2 in pCas-Guide vector
- KN314060D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Vieira, A.R., et al. Genet. Med. 10(9): 668-674 (2008)
Middelhaufe, S., et al. Biochem. J. 407(2): 199-205 (2007)
Kobayashi, T., et al. Mol. Cell. Biol. 26(3): 898-911 (2006)
Zollo, M., et al. Clin. Cancer Res. 11(1): 199-205 (2005)
Forus, A., et al. Oncogene 20(47): 6881-6890 (2001)
Reymond, A., et al. Oncogene 18(51): 7244-7252 (1999)
Immunofluorescence of purified MaxPab antibody to PRUNE on HeLa cell. [antibody concentration 10 ug/ml]
Western Blot analysis of PRUNE expression in transfected 293T cell line (H00058497-T01) by PRUNE MaxPab polyclonal antibody.Lane 1: PRUNE transfected lysate(49.83 KDa).Lane 2: Non-transfected lysate.
PRUNE MaxPab polyclonal antibody. Western Blot analysis of PRUNE expression in NIH/3T3.
PRUNE MaxPab polyclonal antibody. Western Blot analysis of PRUNE expression in Raw 264.7.
Immunohistochemistry analysis in prostate carcinoma (Formalin-fixed, Paraffin-embedded) using PRUNE antibody Cat.-No. AP53474PU-N (C-term), followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PRUNE Antibody (C-term) for IHC; Clinical relevance has not been evaluated.
Western blot analysis of PRUNE (arrow) in Jurkat cell line lysates (35ug/lane) using PRUNE antibody Cat.-No. AP53474PU-N (C-term).
Flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram) using PRUNE antibody Cat.-No. AP53474PU-N, followed by FITC-conjugated goat-anti-rabbit secondary antibodies.
Human Small Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE)
Western Blot analysis of PRUNE expression in transfected 293T cell line by PRUNE monoclonal antibody, clone 1C11.
Host: Rabbit; Target Name: PRUNE; Sample Tissue: COLO205 Whole Cell lysates; Antibody Dilution: 1.0 ug/ml
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| AM31933PU-N | PRUNE antibody |
||||||
 |
Mouse | IgG2a | 1C11 | Purified | Hu | E, P, WB |
50 µg /
€475.00
|
| OriGene Technologies GmbH | |||||||
 +1 additional image |
|||||||
| AP53474PU-N | PRUNE (C-term) antibody |
||||||
 |
Rabbit | Ig | Aff - Purified | Hu | F, P, WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies GmbH | |||||||
| +2 additional images |
|||||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY402855 | PRUNE overexpression lysate |
|
 |
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. |
Rat Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RR204273L2V | Lenti ORF particles, Prune (GFP-tagged ORF) - Rat prune homolog (Drosophila) (Prune), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,120.00
|
|
| OriGene Technologies, Inc. | ||
| RR204273L1V | Lenti ORF particles, Prune (Myc-DDK-tagged ORF) - Rat prune homolog (Drosophila) (Prune), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,000.00
|
|
| OriGene Technologies, Inc. | ||