Switch to Europe/Worldwide website Switch to German website Switch to French website Switch to OriGene Technologies, Inc. (North America)
Home » Target » PSAP antibody

PSAP antibody

(reset)

Principal name

PSAP antibody

Alternative names for PSAP antibody

Prosaposin, SAP1, GLBA, Proactivator polypeptide

SwissProt ID

O13035 (Chick), P07602 (Human), P10960 (Rat), P20097 (Cavpo), P26779 (Bovin), P81405 (Pig), Q61207 (Mouse), Q6IBQ6 (Human)

Gene ID

5660 (PSAP), 19156 (Psap)

Ncbi ID

9606, NP_001035930, NP_001035930.1, NM_001042465, NM_001042466, NP_001139592.1, NM_001146121, NM_001146122, NM_001146123, NM_001146124

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Immunoprecipitation (IP), Frozen Sections (C), Immunocytochemistry/Immunofluorescence (ICC/IF), Flow Cytometry (F)

Background of PSAP antibody

The PSAP gene encodes prosaposin, a precursor of four small nonenzymatic glycoproteins termed 'sphingolipid activator proteins' (SAPs) that assist in the lysosomal hydrolysis of sphingolipids. After proteolytic processing of the presaposin protein, these 4 released polypeptides are functiol activators. Saposin A is encoded by residues 60 to 143 of PSAP, saposin B by 195 to 275, saposin C by 311 to 390, and saposin D by 405 to 487. They are four 12-14 kDa heatstable glycoproteins. Saposins A-D localize primarily to the lysosomal compartment where they facilitate the catabolism of glycosphingolipids with short oligosaccharide groups. Saposins A-D are required for the hydrolysis of certain sphingolipids by specific lysosomal hydrolases. Defects in PSAP are the cause of Gaucher disease, Tay-Sachs disease, and metachromatic leukodystrophy. This PSAP antibody (10801-1-AP) is expected to recognize both saposin A and B.

H00005660-M01 - PSAP
Immunoperoxidase of monoclonal antibody to PSAP on formalin-fixed paraffin-embedded human spleen tissue. [antibody concentration 5ug/ml]
H00005660-M01 - PSAP
Western Blot analysis of PSAP expression in transfected 293T cell line by PSAP monoclonal antibody (M01), clone 1D1-C12.Lane 1: PSAP transfected lysate(58.1 KDa).Lane 2: Non-transfected lysate.
H00005660-B01 - PSAP
Western Blot analysis of PSAP expression in transfected 293T cell line (H00005660-T01) by PSAP MaxPab polyclonal antibody.Lane 1: PSAP transfected lysate(57.64 KDa).Lane 2: Non-transfected lysate.
H00005660-B01 - PSAP
PSAP MaxPab polyclonal antibody. Western Blot analysis of PSAP expression in HepG2 .
H00005660-D01P - PSAP
Western Blot analysis of PSAP expression in transfected 293T cell line (H00005660-T01) by PSAP MaxPab polyclonal antibody.Lane 1: PSAP transfected lysate(58.10 KDa).Lane 2: Non-transfected lysate.
PAB12733 - PSAP
The whole cell lysate derived from human hepatoma HepG2 was immunoblotted PSAP polyclonal antibody ( Cat # PAB12733 ) at 1 : 500.
AP17729PU-N - PSAP
Western blot analysis of SAP Antibody (Center E300) (AP17729PU-N) in Y79,A2058,CEM cell line lysates(35ug/lane). SAP (arrow) was detected using the purified Pab.
C17862-1 - PSAP
Western blot analysis of extracts from NIH-3T3/RAW264.7 cells, using PSAP Antibody. The lane on the right is treated with the synthesized peptide.
AM31026PU-N - PSAP
Human Adrenal: Formalin-Fixed, Paraffin-Embedded (FFPE)
AM31026PU-N - PSAP
Western Blot analysis of PSAP expression in transfected 293T cell line by PSAP monoclonal antibody clone 1D1-C12.
AM39077PU-S - PSAP
Immunohistochemistry of Paraffin-embedded pancreas tissue using anti-PSAP antibody cat.no. AM39077PU-S with DAB staining.
AM39077PU-S - PSAP
Immunohistochemistry of Formalin-Fixed, Paraffin-Embedded (FFPE)  human kidney tissue using anti-PSAP antibody cat.no. AM39077PU-S
AM39077PU-S - PSAP
Immunofluorescence of HepG2 cells using anti-PSAP antibody cat.no. AM39077PU-S (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have beenlabeled with Alexa Fluor-555 phalloidin.
AM39077PU-S - PSAP
Western blot of anti-PSAP antibody cat.no. AM39077PU-S against HEK293 (lane 1) and PSAP (aa325-524)-hIgGFc transfected HEK293 cell lysate (lane 2).
AM39077PU-S - PSAP
Western blot of anti-PSAP antibody cat. no. AM39077PU-S against human PSAP recombinant protein. (Expected MW is 47.8 kDa).
AM39077PU-S - PSAP
ELISA used with anti-PSAP antibody cat.no. AM39077PU-S:
Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng)
AM39077PU-S - PSAP
Flow cytometry of HeLa cells using anti-PSAP antibody cat.no.AM39077PU-S (green) and negative control (purple).
TA308630 - PSAP
Sample (30 ug of whole cell lysate). A: Molt-4 . 7.5% SDS PAGE. TA308630 diluted at 1:2000.
TA308630 - PSAP
Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft , using PSAP(TA308630) antibody at 1:500 dilution.
TA323294 - PSAP
Predicted band size: 58 kDa . Positive control: HEK-293 cell lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lysate: 40 ug Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute)
TA327188 - PSAP
Western blot analysis of extracts of HEK-293 cell lines , using PSAP antibody.
TA327188 - PSAP
Immunofluorescence analysis of MCF7 cell using PSAP antibody. Blue: DAPI for nuclear staining.
TA327730 - PSAP
Immunohistochemistry staining of Paraffin Prostate tissue by PSAP antibody (dilution: 1:25 - 1:100; visualization of staining: Cytoplasmic)
  1. Quick-Filters:
  2. Highly Validated (1)

10 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

PSAP (325-524) antibody
Immunohistochemistry of Paraffin-embedded pancreas tissue using anti-PSAP antibody cat.no. AM39077PU-S with DAB staining. Mouse IgG1 4D5F4 Aff - Purified Hu E, F, ICC/IF, P, WB
50 µg / €425.00
  OriGene Technologies GmbH
+6 additional images

Highly validated antibody!

PSAP antibody
  Rabbit polyclonal antibody to PSAP (prosaposin)  
Sample (30 ug of whole cell lysate). A: Molt-4 . 7.5% SDS PAGE. TA308630 diluted at 1:2000. Rabbit IgG Aff - Purified Hu P, WB
0.1 ml / €415.00
  OriGene Technologies, Inc.
+1 additional image

PSAP antibody
  Anti-PSAP Rabbit Polyclonal Antibody  
Predicted band size: 58 kDa . Positive control: HEK-293 cell lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lysate: 40 ug Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €335.00
  OriGene Technologies, Inc.

PSAP antibody
  Rabbit anti-PSAP Polyclonal Antibody  
Western blot analysis of extracts of HEK-293 cell lines , using PSAP antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €335.00
  OriGene Technologies, Inc.
+1 additional image

PSAP antibody
  Mouse Monoclonal PSAP Antibody  
Immunohistochemistry staining of Paraffin Prostate tissue by PSAP antibody (dilution: 1:25 - 1:100; visualization of staining: Cytoplasmic) Mouse IgG1 PASE/4LJ Purified Hu P
1 ml / €370.00
  OriGene Technologies, Inc.

PSAP (Center) antibody
Western blot analysis of SAP Antibody (Center E300) (AP17729PU-N) in Y79,A2058,CEM cell line lysates(35ug/lane). SAP (arrow) was detected using the purified Pab. Rabbit Hu WB
0.4 ml / €415.00
  OriGene Technologies GmbH

PSAP antibody
PSAP Rabbit Purified Hu C, E, WB
0.1 mg / €450.00
  OriGene Technologies GmbH

Lysates

Catalog No.    

PSAP overexpression lysate
PSAP overexpression lysate  
0.1 mg / €495.00
  OriGene Technologies, Inc.

PSAP overexpression lysate
PSAP overexpression lysate  
0.1 mg / €495.00
  OriGene Technologies, Inc.

PSAP overexpression lysate
PSAP overexpression lysate  
0.1 mg / €315.00
  OriGene Technologies, Inc.

10 Item(s)

per page

Available Filters

Product Type
  1. Lysate (3)
  2. Primary Antibody (7)
Clonality
  1. Polyclonal (5)
  2. Monoclonal (2)
Target
  1. PSAP (7)
Property
  1. 325-524 (1)
  2. Center (1)
Reactivity
  1. Human (Hu) (7)
  2. Mouse (Ms) (2)
  3. Rat (Rt) (2)
Applications
  1. Western blot / Immunoblot (WB) (5)
  2. Paraffin Sections (P) (3)
  3. Enzyme Immunoassay (E) (2)
  4. Frozen sections (C) (1)
  5. Flow cytometry (F) (1)
  6. Immunocytochemistry/​Immunofluorescence (ICC/IF) (1)
Presentation
  1. Purified (4)
  2. Aff - Purified (2)
Host
  1. Rabbit (5)
  2. Mouse (2)
Images
  1. 1 image (6)
  2. 3+ images (1)
  3. 5+ images (1)
Images: Applications
  1. Western blot / Immunoblot (WB) (5)
  2. IHC (2)
  3. Enzyme Immunoassay (E) (1)
  4. Flow cytometry (F) (1)
  5. Immunocytochemistry/​Immunofluorescence (ICC/IF) (1)
  6. Immunofluorescence (IF) (1)
  7. Paraffin Sections (P) (1)
Manufacturer
  1. OriGene Technologies (10)
Service
  • OriGene Technologies GmbH
  • Schillerstraße 5
  • D-32052 Herford
  • Germany
  • phone: +49-5221-34606-0
  • free call: 0800-227 47 46 (Germany only)
  • fax: +49-5221-34606-11
  • email: info-de@origene.com
  • orders: ordering-de@origene.com
 
© Copyright 1998 - 2018 OriGene Technologies GmbH
  • LinkedIn