PSCA antibody
Principal name
PSCA antibody
Alternative names for PSCA antibody
Prostate stem cell antigen
Gene ID
8000 (PSCA)
Ncbi ID
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection))
Background of PSCA antibody
Prostate stem cell antigen (PSCA) is a 123 amino acid glycosylated protein that shares homology with the Thy-1/Ly-6 family of glycosyl-phosphatidylinositol GPI)-anchored cell surface antigens. The PSCA gene maps to chromosome 8q24.2 and transcripts are most prevalent in prostate and placenta. The gene encoding c-myc is also located on chromosome 8q and like PSCA, is overexpressed in a large number of prostate cancers. Transcripts for PSCA are also abundant in urothelial tumors, and levels of PSCA transcripts increase in confluent RT112 bladder carcinomas, suggesting that PSCA is a marker for urothelial and gastric tissue carcinogenesis. Among prostate cancer cell surface antigens, PSCA is expressed in over 80% of prostate carcinomas and correlates well to certain prostate cancer phenotypes such as prostate cancer bone metastates.
GTX15168 staining PSCA in human breast carcinoma by Immunohistochemistry (FFPE-sections).
Human Prostate Carcinoma stained with anti-Prostate Stem Cell Antigen (PSCA) antibody (GTX15168)
Human Prostate Carcinoma stained with anti-Prostate Stem Cell Antigen (PSCA) antibody (GTX15168)
GTX15169 staining PSCA in human breast carcinoma by Immunohistochemistry (FFPE-sections)
Human Prostate Carcinoma stained with anti-Prostate Stem Cell Antigen (PSCA) antibody (GTX73982)
Western blot analysis of Prostate Stem Cell Antigen antibody (Cat.-No.: AP06293PU-N) in extracts from HepG2 cells.
Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with PSCA polyclonal antibody ( Cat # PAB1868 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with PSCA antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
AP15749PU PSCA antibody staining of Human Breast Carcinoma.
AP15749PU PSCA antibody staining of Human Prostate Adenocarcinoma.
Western Blot analysis of PSCA expression in transfected 293T cell line (H00008000-T01) by PSCA MaxPab polyclonal antibody.Lane 1: PSCA transfected lysate(13.53 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of PSCA expression in transfected 293T cell line (H00008000-T02) by PSCA MaxPab polyclonal antibody.Lane 1: PSCA transfected lysate(12.90 KDa).Lane 2: Non-transfected lysate.
Immunohistochemical staining of PSCA on formalin fixed, paraffin embedded human breast cancer with PSCA polyclonal antibody.
Western Blot: PSCA Lysate [NBL1-14856] - Western Blot experiments. Left-Control; Right -Over-expression Lysate for PSCA.
PC-3 cells were subjected to SDS PAGE followed by western blot with 17171-1-AP(PSCA antibody) at dilution of 1:500
ELISA: PSCA Antibody [32500002]
Human 721_B; WB Suggested Anti-PSCA Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: 721_B cell lysate; PSCA antibody - C-terminal region (ARP42250_P050) in Human 721_B cells using Western Blot
IHC Image for AP22700PU-NHuman Prostate (formalin-fixed, paraffin-embedded) stained with PSCA antibody AP22700PU-N at 10 µg/ml followed by biotinylated goat anti-rabbit IgG secondary antibody LS-D1, alkaline phosphatase-streptavidin and chromogen.
IHC Image for AP22700PU-NHuman Placenta (formalin-fixed, paraffin-embedded) stained with PSCA antibody AP22700PU-N at 10 µg/ml followed by biotinylated goat anti-rabbit IgG secondary antibody LS-D1, alkaline phosphatase-streptavidin and chromogen.
IHC Image for AP22700PU-NHuman breast ca. stained with Anti-PSCA antibody
Formalin-fixed and paraffin embedded rat testis labeled with Rabbit Anti PSCA Polyclonal Antibody, Unconjugated (bs-0669R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti PSCA Polyclonal Antibody, Unconjugated (bs-0669R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Western blot analysis of PSCA expression in DU145 (A); human prostate (B) whole cell lysates.
ELISA: PSCA Antibody
Western blot analysis of extracts from mouse prostate, using Prostate Stem Cell Antigen antibody (#TA314711).The lane on the right is treated with the synthesized peptide.
Predicted band size: 13 kDa . Positive control: Mouse bladder tissue lysate. Recommended dilution: 1/500-2000. (Gel: 12%SDS-PAGE Lysate: 40 ug per lane Primary antibody: 1/450 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 40 seconds)
Predicted cell location: Cytoplasm. Positive control: Human colon cancer tissue. Recommended dilution: 1/25-100 The image on the left is immunohistochemistry of paraffin-embedded human colon cancer tissue using PSCA antibody at dilution 1/30, on the right is treated with the synthetic peptide. (Original magnification:x200)
Western blot analysis of extracts of various cell lines, using PSCA antibody.
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA314711 | PSCA antibody |
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| Rabbit polyclonal Prostate Stem Cell Antigen antibody | |||||||
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Rabbit | IgG | Purified | Hu, Ms | E, WB |
0.1 ml /
€335.00
|
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| OriGene Technologies, Inc. | |||||||
| TA332789 | PSCA antibody |
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| Rabbit anti-PSCA Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms |
0.1 mg /
€335.00
|
||
| OriGene Technologies, Inc. | |||||||
| TA590594 | PSCA antibody |
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| Rabbit Polyclonal PSCA Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu | E |
0.1 mg /
€409.00
|
|
| OriGene Technologies, Inc. | |||||||
| AP06293PU-N | PSCA antibody |
||||||
 |
Rabbit | Aff - Purified | Hu, Ms | E, WB |
0.1 mg /
€370.00
|
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| OriGene Technologies GmbH | |||||||
| AP11380PU-N | PSCA (Center) antibody |
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 |
Rabbit | Ig | Purified | Hu | E, P, WB |
0.4 ml /
€415.00
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|
| OriGene Technologies GmbH | |||||||
 +1 additional image |
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