PSMA4 antibody
Principal name
PSMA4 antibody
Alternative names for PSMA4 antibody
Proteasome subunit alpha type-4, Macropain subunit C9, Proteasome component C9, HC9, PSC9, Proteasome subunit L, Multicatalytic endopeptidase complex subunit C9
SwissProt ID
O81148 (Arath), P18053 (Drome), P21670 (Rat), P23638 (Yeast), P25789 (Human), Q09682 (Schpo), Q3ZCK9 (Bovin), Q9N599 (Caeel), Q9R1P0 (Mouse)
Ncbi ID
9606, NP_001096137, NP_001096137.1, NM_001102667, NM_001102668, NP_002780, NP_036096.1, NM_002789
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Frozen Sections (C), Paraffin Sections (P), Flow Cytometry (F)
Background of PSMA4 antibody
- Kit Component:
- - KN314092G1, Psma4 gRNA vector 1 in pCas-Guide vector
- KN314092G2, Psma4 gRNA vector 2 in pCas-Guide vector
- KN314092D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Kristensen P. et al. (1995) Biochem. Biophys. Res.Commun. 205: 1785-1789.Morimoto Y. et al. (1995) J. Biochem. 117: 471-474
Immunofluorescence of monoclonal antibody to PSMA4 on HeLa cell. [antibody concentration 10 ug/ml]
PSMA4 monoclonal antibody (M01), clone 2A10-E4 Western Blot analysis of PSMA4 expression in Hela ( Cat # L013V1 ).
Detection limit for recombinant GST tagged PSMA4 is approximately 3ng/ml as a capture antibody.
Human Heart; WB Suggested Anti-PSMA4 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Human heart; PSMA4 antibody - N-terminal region (ARP56312_P050) in Human Heart cells using Western Blot
AP21301PU-N PSMA4 antibody staining of K562 cell lysate at 1µg/ml (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
AP21301PU-N PSMA4 antibody staining of NIH3T3 (A), Mouse Spleen (B), Rat Spleen (C)) lysates at 0.3 µg/ml (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-PSMA4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504023)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-PSMA4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504023)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-PSMA4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504023)
Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-PSMA4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504023)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-PSMA4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504023)
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PSMA4 monoclonal antibody.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PSMA4 (RC202786, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PSMA4.
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-PSMA4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504058)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-PSMA4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504058)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-PSMA4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504058)
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-PSMA4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504058)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-PSMA4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504058)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PSMA4 (RC202786, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PSMA4.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PSMA4 monoclonal antibody.
Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-PSMA4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504059)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-PSMA4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504059)
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-PSMA4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504059)
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-PSMA4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504059)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-PSMA4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504059)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-PSMA4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504059)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PSMA4 (RC202786, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PSMA4.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PSMA4 monoclonal antibody.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PSMA4 (RC202786, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PSMA4.
Flow cytometric Analysis of Jurkat cells, using anti-PSMA4 antibody(TA504163),(Red), compared to a nonspecific negative control antibody,(Blue).
Western blot analysis of extracts of various cell lines, using PSMA4 antibody.
Immunohistochemistry of paraffin-embedded human thyroid cancer using PSMA4 antibody at dilution of 1:100 (400x lens).
Immunohistochemistry of paraffin-embedded rat lung using PSMA4 antibody at dilution of 1:100 (200x lens).
Immunofluorescence analysis of U2OS cell using PSMA4 antibody. Blue: DAPI for nuclear staining
WB Suggested Anti-PSMA4 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: Human heart
- Quick-Filters:
- Highly Validated (4)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA332532 | PSMA4 antibody |
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| Rabbit anti-PSMA4 Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt |
0.1 mg /
€335.00
|
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| OriGene Technologies, Inc. | |||||||
 +3 additional images |
Highly validated antibody! |
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| TA504023 | PSMA4 antibody |
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| PSMA4 mouse monoclonal antibody, clone OTI1H10 (formerly 1H10) | |||||||
 |
Mouse | IgG1 | OTI1H10 | Purified | Can, Hu, Mky, Ms, Rt | P, WB |
0.1 ml /
€379.00
|
| OriGene Technologies, Inc. | |||||||
| +6 additional images |
Highly validated antibody!
|
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| TA504058 | PSMA4 antibody |
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| PSMA4 mouse monoclonal antibody, clone OTI5D8 (formerly 5D8) | |||||||
 |
Mouse | IgG2b | OTI5D8 | Purified | Can, Hu, Mky, Ms, Rt | P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +6 additional images |
Highly validated antibody!
|
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| TA504059 | PSMA4 antibody |
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| PSMA4 mouse monoclonal antibody, clone OTI4B8 (formerly 4B8) | |||||||
 |
Mouse | IgG1 | OTI4B8 | Purified | Hu, Mky, Ms, Rt | P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +7 additional images |
Highly validated antibody!
|
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| AP21301PU-N | PSMA4 antibody |
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 |
Goat | Aff - Purified | Can, Hu, Ms, Rt | E, WB |
0.1 mg /
€380.00
|
||
| OriGene Technologies GmbH | |||||||
| +1 additional image |
OriGene recommends this product for Western blotting. |
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| TA504163 | PSMA4 antibody |
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| PSMA4 mouse monoclonal antibody, clone OTI2D12 (formerly 2D12) | |||||||
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Mouse | IgG1 | OTI2D12 | Purified | Hu | F, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|
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| AP55207SU-N | PSMA4 antibody |
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Rabbit | Serum | Hu | C, E, WB |
0.2 ml /
€430.00
|
||
| OriGene Technologies GmbH | |||||||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP302786 | PSMA4 (transcript variant 1) |
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| Recombinant protein of human proteasome (prosome, macropain) subunit, alpha type, 4 (PSMA4), transcript variant 1 | |||||
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Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
| TP320385 | PSMA4 (transcript variant 2) |
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| Recombinant protein of human proteasome (prosome, macropain) subunit, alpha type, 4 (PSMA4), transcript variant 2 | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY420186 | PSMA4 overexpression lysate |
|
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0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. | ||
| LY420187 | PSMA4 overexpression lysate |
|
|
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. | ||
| LY426193 | PSMA4 overexpression lysate |
|
|
0.1 mg /
€315.00
|
|
| OriGene Technologies, Inc. | ||
| LY426194 | PSMA4 overexpression lysate |
|
|
0.1 mg /
€315.00
|
|
| OriGene Technologies, Inc. |
