PSMA6 antibody
Principal name
PSMA6 antibody
Alternative names for PSMA6 antibody
Proteasome iota chain, Macropain iota chain, PROS27, p27K, Multicatalytic endopeptidase complex iota chain, 27 kDa prosomal protein, Proteasome subunit alpha type-6
Ncbi ID
9606, NP_002782, NP_002782.1, NM_002791, NP_036098.1, NM_011968
Available reactivities
Available hosts
Available applications
Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Flow Cytometry (F), Paraffin Sections (P)
Background of PSMA6 antibody
The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. PSMA6 is a member of the peptidase T1A family, which is a 20S core alpha subunit.The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the peptidase T1A family, that is a 20S core alpha subunit. A pseudogene has been identified on the Y chromosome. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
General readings
Nandi D., et al. (2006) J Biosci. 31:137-155.Kristensen P., et al. (1995) Biochem Biophys Res Commun. 205:1785-1789.
Human Hela; Host:Rabbit. Target Name:PSMA6. Sample Tissue:Human Hela. Antibody Dilution: 1.0ug/ml. PSMA6 is supported by BioGPS gene expression data to be expressed in HeLa.; PSMA6 antibody - N-terminal region (ARP40419_P050) in Human Hela cells using Western Blot
Human Jurkat; WB Suggested Anti-PSMA6 Antibody Titration: 0.2-1 ug/ml. Positive Control: Jurkat cell lysate; PSMA6 antibody - N-terminal region (ARP40419_P050) in Human Jurkat cells using Western Blot
Immunofluorescence of purified MaxPab antibody to PSMA6 on HeLa cell. [antibody concentration 10 ug/ml]
PSMA6 MaxPab polyclonal antibody. Western Blot analysis of PSMA6 expression in human liver.
Western Blot analysis of PSMA6 expression in transfected 293T cell line (H00005687-T01) by PSMA6 MaxPab polyclonal antibody.Lane 1: PSMA6 transfected lysate(27.06 KDa).Lane 2: Non-transfected lysate.
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-PSMA6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800101)
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-PSMA6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800101)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-PSMA6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800101)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-PSMA6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800101)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-PSMA6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800101)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-PSMA6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800101)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-PSMA6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800101)
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-PSMA6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800101)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-PSMA6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800101)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-PSMA6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800101)
Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-PSMA6 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PSMA6 (RC209976, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PSMA6.
HEK293T cells transfected with either RC209976 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-PSMA6 antibody(TA800101), and then analyzed by flow cytometry.
Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-PSMA6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800102)
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-PSMA6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800102)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-PSMA6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800102)
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-PSMA6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800102)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-PSMA6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800102)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-PSMA6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800102)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-PSMA6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800102)
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-PSMA6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800102)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-PSMA6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800102)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-PSMA6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800102)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-PSMA6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800102)
Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-PSMA6 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PSMA6 (RC209976, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PSMA6.
HEK293T cells transfected with either RC209976 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-PSMA6 antibody(TA800102), and then analyzed by flow cytometry.
Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-PSMA6 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PSMA6 (RC209976, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PSMA6.
HEK293T cells transfected with either RC209976 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-PSMA6 antibody(TA800103), and then analyzed by flow cytometry.
Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-PSMA6 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PSMA6 (RC209976, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PSMA6.
HEK293T cells transfected with either RC209976 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-PSMA6 antibody(TA800104), and then analyzed by flow cytometry.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PSMA6 (RC209976, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PSMA6.
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-PSMA6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800107)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-PSMA6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800107)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-PSMA6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800107)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-PSMA6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800107)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-PSMA6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800107)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PSMA6 (RC209976, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PSMA6.
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-PSMA6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA800108)
Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-PSMA6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA800108)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PSMA6 (RC209976, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PSMA6.
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-PSMA6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800109)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PSMA6 (RC209976, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PSMA6.
Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-PSMA6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800122)
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-PSMA6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800122)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-PSMA6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800122)
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-PSMA6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800122)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-PSMA6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800122)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-PSMA6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800122)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-PSMA6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800122)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-PSMA6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800122)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-PSMA6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800122)
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-PSMA6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800122)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-PSMA6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800122)
Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-PSMA6 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PSMA6 (RC209976, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PSMA6.
HEK293T cells transfected with either RC209976 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-PSMA6 antibody(TA800122), and then analyzed by flow cytometry.
Sample(30 ug whole cell lysate). A:Hep G2 . 7.5% SDS PAGE. TA308942 diluted at 1:500
Sample (50 ug of whole cell lysate). A: Mouse brain. 12% SDS PAGE. TA308942 diluted at 1:1000.
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using 20S Proteasome alpha 6(TA308942) antibody at 1:200 dilution.
Sample (30 ug of whole cell lysate). A: 293T. B: A431 . C: H1299. D: Hela. 12% SDS PAGE. TA308943 diluted at 1:1000.
Proteasome 20S alpha 6 antibody detects PSMA6 protein by Western blot analysis. A. 50 ug mouse liver lysate/extract. 12 % SDS-PAGE. Proteasome 20S alpha 6 antibody (TA308943) dilution: 1:1000
Proteasome 20S alpha 6 antibody detects PSMA6 protein by Western blot analysis. A. 50 ug rat liver lysate/extract. 12 % SDS-PAGE. Proteasome 20S alpha 6 antibody (TA308943) dilution: 1:1000
Immunohistochemical analysis of paraffin-embedded H1299 xenograft , using 20S Proteasome alpha6 (TA308943) antibody at 1:100 dilution.
Proteasome 20S alpha 6 antibody detects Proteasome 20S alpha 6 protein at cytosol and nucleus on mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. Proteasome 20S alpha 6 antibody (TA308943) dilution: 1:500.
Proteasome 20S alpha 6 antibody detects Proteasome 20S alpha 6 protein at cytosol and nucleus on mouse urinary bladder by immunohistochemical analysis. Sample: Paraffin-embedded mouse urinary bladder. Proteasome 20S alpha 6 antibody (TA308943) dilution: 1
Proteasome 20S alpha 6 antibody detects Proteasome 20S alpha 6 protein at cytosol and nucleus on mouse lung by immunohistochemical analysis. Sample: Paraffin-embedded mouse lung. Proteasome 20S alpha 6 antibody (TA308943) dilution: 1:500.
Immunofluorescence analysis of paraformaldehyde-fixed A431, using 20S Proteasome alpha6 (TA308943) antibody at 1:200 dilution.
Predicted band size: 27 kDa . Positive control: Human brain tissue lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lysate: 40 ug Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute)
Western blot analysis of extracts of various cell lines, using PSMA6 antibody.
Immunohistochemistry of paraffin-embedded human rectal cancer using PSMA6 antibody at dilution of 1:200 (400x lens).
Immunohistochemistry of paraffin-embedded human stomach cancer using PSMA6 antibody at dilution of 1:200 (400x lens).
Immunofluorescence analysis of U2OS cell using PSMA6 antibody. Blue: DAPI for nuclear staining.
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- Highly Validated (2)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA308943 | PSMA6 antibody |
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| Rabbit Polyclonal antibody to Proteasome 20S alpha 6 (proteasome (prosome, macropain) subunit, alpha type, 6) | |||||||
 |
Rabbit | IgG | Aff - Purified | Hu, Ms, Rt | ICC/IF, P, WB |
0.1 ml /
€415.00
|
|
| OriGene Technologies, Inc. | |||||||
 +7 additional images |
Highly validated antibody! |
||||||
| TA327325 | PSMA6 antibody |
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| PSMA6 Rabbit Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt |
0.1 mg /
€335.00
|
||
| OriGene Technologies, Inc. | |||||||
| +3 additional images |
Highly validated antibody! |
||||||
| TA308942 | PSMA6 antibody |
||||||
| Rabbit Polyclonal antibody to Proteasome 20S alpha 6 (proteasome (prosome, macropain) subunit, alpha type, 6) | |||||||
 |
Rabbit | IgG | Aff - Purified | Hu, Ms | ICC/IF, WB |
0.1 ml /
€415.00
|
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
|||||||
| TA322959 | PSMA6 antibody |
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| Anti-PSMA6 Rabbit Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||