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PSMD12 antibody

Principal name

PSMD12 antibody

Alternative names for PSMD12 antibody

26S proteasome regulatory subunit p55, RPN5, 26S proteasome non-ATPase regulatory subunit 12, 26S proteasome regulatory subunit RPN5

SwissProt ID

O00232 (Human), Q2KJ25 (Bovin), Q9D8W5 (Mouse)

Gene ID

5718 (PSMD12)

Ncbi ID

9606, NP_002807.1, NM_002816, NM_174871

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Rb (Rabbit), Ze (Zebrafish)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P)

Background of PSMD12 antibody

The 26S proteasome is a multicatalytic proteise complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a non-ATPase subunit of the 19S regulator. A pseudogene has been identified on chromosome 3.

Western blot analysis of extracts from HepG2 cells, using PSMD12 Antibody. The lane on the right is treated with the synthesized peptide.

Human HCT15; WB Suggested Anti-PSMD12 Antibody Titration: 0.2-1 ug/ml. Positive Control: HCT15 cell lysate. PSMD12 is supported by BioGPS gene expression data to be expressed in HCT15.; PSMD12 antibody - N-terminal region (ARP57839_P050) in Human HCT15 cells using Western Blot

ARP57839_P050

Immunohistochemical analysis of PSMD12 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Western blot analysis of PSMD12 expression in A431 (A); COLO320 (B); HeLa (C) whole cell lysates.

WB Suggested Anti-PSMD12 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:62500; Positive Control: Hela cell lysatePSMD12 is supported by BioGPS gene expression data to be expressed in HeLa

TA344574

WB Suggested Anti-PSMD12 Antibody Titration: 0.2-1 ug/ml; Positive Control: HCT15 cell lysatePSMD12 is supported by BioGPS gene expression data to be expressed in HCT15

TA344575

Lysates

Catalog No.
LY430412	PSMD12 overexpression lysate
		0.1 mg / €315.00
		OriGene Technologies, Inc.

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