PSMD14 antibody
Principal name
PSMD14 antibody
Alternative names for PSMD14 antibody
POH1, 26S proteasome non-ATPase regulatory subunit 14, 26S proteasome regulatory subunit RPN11, 26S proteasome-associated PAD1 homolog 1
Ncbi ID
NP_005796, 9606, NP_005796.1, NP_067501.2, NM_021526, NM_005805
Available reactivities
Available hosts
Available applications
Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), ELISA (detection) (E(detection)), Paraffin Sections (P)
Background of PSMD14 antibody
- Kit Component:
- - KN314120G1, Psmd14 gRNA vector 1 in pCas-Guide vector
- KN314120G2, Psmd14 gRNA vector 2 in pCas-Guide vector
- KN314120D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Spataro,V., et al., (1997) J. Biol. Chem. 272 (48), 30470-30475
Human HeLa, Mouse liver; . Lanes: 1. 100 ug HeLa cell lysate. 2. 100 ug mouse liver cytosolic extract 3. 1.5 ug human 26S Proteasome. Primary Antibody Dilution: 1:1000. Secondary Antibody: Anti-Rabbit AP. Secondary Antibody Dilution: 1:10000. Gene Name: PSMD14. Submitted by: Anonymous.; PSMD14 antibody - C-terminal region (ARP32449_P050) in Human HeLa, Mouse liver cells using Western Blot
Human HepG2; WB Suggested Anti-PSMD14 Antibody Titration: 0.2-1 ug/ml. Positive Control: HepG2 cell lysate. PSMD14 is supported by BioGPS gene expression data to be expressed in HepG2.; PSMD14 antibody - C-terminal region (ARP32449_P050) in Human HepG2 cells using Western Blot
Immunofluorescence of monoclonal antibody to PSMD14 on A-431 cell. [antibody concentration 25 ug/ml]
PSMD14 monoclonal antibody (M01), clone 4A10-E8 Western Blot analysis of PSMD14 expression in A-431 ( Cat # L015V1 ).
Detection limit for recombinant GST tagged PSMD14 is approximately 0.03ng/ml as a capture antibody.
Human Lung; . Rabbit Anti-PSMD14 antibody. . Catalog Number:. ARP32450_T100 . Paraffin Embedded Tissue: Human Lung cell . Cellular Data: Epithelial cells of renal tubule. Antibody Concentration: 4.0-8.0 ug/ml. Magnification: 400X; PSMD14 antibody (ARP32450_T100) in Human Lung cells using Immunohistochemistry
Human HepG2; WB Suggested Anti-PSMD14 Antibody Titration: 1.25ug/ml. ELISA Titer: 1:12500. Positive Control: HepG2 cell lysate. PSMD14 is supported by BioGPS gene expression data to be expressed in HepG2.; PSMD14 antibody - N-terminal region (ARP32450_T100) in Human HepG2 cells using Western Blot
Immunofluorescence of purified MaxPab antibody to PSMD14 on HeLa cell. [antibody concentration 10 ug/ml]
Western Blot analysis of PSMD14 expression in transfected 293T cell line (H00010213-T02) by PSMD14 MaxPab polyclonal antibody.Lane 1: PSMD14 transfected lysate(34.1 KDa).Lane 2: Non-transfected lysate.
Predicted band size: 35 kDa . Positive control: NIH/3T3 and 231 cell lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lane1: NIH/3T3 cell lysate Lane2: 231 cell lysate Lysates: 50 ug per lane Primary antibody: 1/300 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute)
Predicted cell location: Cytoplasm,Nucleus. Positive control: Human liver cancer tissue . Recommended dilution: 1/25-100 The image on the left is immunohistochemistry of paraffin-embedded human liver cancer tissue using PSMD14 antibody at dilution 1/25, on the right is treated with the fusion protein. (Original magnification:x200)
Predicted band size: 35 kDa . Positive control: 293T, SKOV3, NIH/3T3 and 231 cell lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lane1: 293T cell lysate Lane2: SKOV3 cell lysate Lane3: NIH/3T3 cell lysate Lane4: 231 cell lysate Lysates: 50 ug per lane Primary antibody: 1/200 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute)
Predicted cell location: Cytoplasm,Nucleus. Positive control: Human liver cancer tissue . Recommended dilution: 1/25-100 The image on the left is immunohistochemistry of paraffin-embedded human liver cancer tissue using PSMD14 antibody at dilution 1/20, on the right is treated with the fusion protein. (Original magnification:x200)
Lanes: ; 1. 100 ug HeLa cell lysate; 2. 100 ug mouse liver cytosolic extract 3. 1.5 ug human 26S Proteasome; Primary Antibody Dilution: ; 1:1000; Secondary Antibody: ; Anti-Rabbit AP; Secondary Antibody Dilution: ; 1:10000; Gene Name: ; PSMD14; Submitted
WB Suggested Anti-PSMD14 Antibody Titration: 0.2-1 ug/ml; Positive Control: HepG2 cell lysatePSMD14 is supported by BioGPS gene expression data to be expressed in HepG2
WB Suggested Anti-PSMD14 Antibody Titration: 1.25ug/ml; ELISA Titer: 1:12500; Positive Control: HepG2 cell lysatePSMD14 is supported by BioGPS gene expression data to be expressed in HepG2
Rabbit Anti-PSMD14 antibody; Paraffin Embedded Tissue: Human Lung cell; Cellular Data: Epithelial cells of renal tubule; Antibody Concentration: 4.0-8.0 ug/ml; Magnification: 400X
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA321727 | PSMD14 antibody |
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| Anti-PSMD14 Rabbit Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, P, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
 +1 additional image |
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| TA321728 | PSMD14 antibody |
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| Anti-PSMD14 Rabbit Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, P, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|||||||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP309829 | PSMD14 |
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| Recombinant protein of human proteasome (prosome, macropain) 26S subunit, non-ATPase, 14 (PSMD14) | |||||
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Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR204368L2V | Lenti ORF particles, Psmd14 (GFP-tagged) - Mouse proteasome (prosome, macropain) 26S subunit, non-ATPase, 14 (Psmd14), 200 uL, >10^7 TU/mL |
|
 |
200 µl /
€930.00
|
|
| OriGene Technologies, Inc. | ||
| MR204368L1V | Lenti ORF particles, Psmd14 (Myc-DDK-tagged) - Mouse proteasome (prosome, macropain) 26S subunit, non-ATPase, 14 (Psmd14), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€830.00
|
|
| OriGene Technologies, Inc. | ||