PTPN1 antibody
Principal name
PTPN1 antibody
Alternative names for PTPN1 antibody
Protein Tyrosin Phosphatase , PTP1B, PTP-1B, Tyrosine-protein phosphatase non-receptor type 1, Protein-tyrosine phosphatase 1B
Ncbi ID
NP_002818.1, 9606, NP_002818, NP_035331.3, NM_011201, NM_012637, NM_002827
Available reactivities
Available hosts
Available applications
Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E), ELISA (detection) (E(detection)), Immunoprecipitation (IP), Flow Cytometry (F), Immunocytochemistry/Immunofluorescence (ICC/IF)
Background of PTPN1 antibody
PTPN1 is the founding member of the protein tyrosine phosphatase (PTP) family. PTPs catalyze the hydrolysis of the phosphate monoesters specifically on tyrosine residues. Members of the PTP family share a highly conserved catalytic motif, which is essential for the catalytic activity. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP has been shown to act as a negative regulator of insulin signaling by dephosphorylating the phosphotryosine residues of insulin receptor kinase. This PTP was also reported to dephosphorylate epidermal growth factor receptor kinase, as well as JAK2 and TYK2 kinases, which implicated the role of this PTP in cell growth control, and cell response to interferon stimulation.The protein encoded by this gene is the founding member of the protein tyrosine phosphatase (PTP) family, which was isolated and identified based on its enzymatic activity and amino acid sequence. PTPs catalyze the hydrolysis of the phosphate monoesters specifically on tyrosine residues. Members of the PTP family share a highly conserved catalytic motif, which is essential for the catalytic activity. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP has been shown to act as a negative regulator of insulin signaling by dephosphorylating the phosphotryosine residues of insulin receptor kinase. This PTP was also reported to dephosphorylate epidermal growth factor receptor kinase, as well as JAK2 and TYK2 kinases, which implicated the role of this PTP in cell growth control, and cell response to interferon stimulation. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
General readings
Chernoff, J.,et al. (1990) Proc. Natl. Acad. Sci.
USA 87, 2735-2739.
Immunohistochemistry (IHC) analyzes of PTP1B antibody (Cat.-No.: AP06575PU-N) in paraffin-embedded human brain tissue.
Western blot (WB) analysis of PTP1B antibody (Cat.-No.: AP06575PU-N) in extracts from COS7 cells treated with UV 30'.
Immunoblot analysis of cell lysates. Lane 1 : HepG2 cell lysate and lane 2 : 293T cell lysate.
PTPN1 MaxPab polyclonal antibody. Western Blot analysis of PTPN1 expression in human spleen.
Western Blot analysis of PTPN1 expression in transfected 293T cell line (H00005770-T01) by PTPN1 MaxPab polyclonal antibody.Lane 1: PTPN1 transfected lysate(47.85 KDa).Lane 2: Non-transfected lysate.
Transfected 293T; WB Suggested Anti-PTPN1 Antibody Titration: 0.2-1 ug/ml. Positive Control: Transfected 293T; PTPN1 antibody - middle region (ARP45360_P050) in Transfected 293T cells using Western Blot
Western blot analysis of anti-PTP1B Antibody in Ramos cell line lysates (35ug/lane). PTP1B (arrow) was detected using the purified Pab.
Western Blot analysis of PTPN1 expression in transfected 293T cell line (H00005770-T01) by PTPN1 MaxPab polyclonal antibody.Lane 1: PTPN1 transfected lysate(50.00 KDa).Lane 2: Non-transfected lysate.
Immunoprecipitation of PTPN1 transfected lysate using anti-PTPN1 MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with PTPN1 MaxPab mouse polyclonal antibody (B01) (H00005770-B01).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PTPN1 (RC204902, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PTPN1.
HEK293T cells transfected with either RC204902 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-PTPN1 antibody(TA503037), and then analyzed by flow cytometry.
Flow cytometric Analysis of Jurkat cells, using anti-PTPN1 antibody(TA503037),(Red), compared to a nonspecific negative control antibody,(Blue).
Flow cytometric Analysis of Hela cells, using anti-PTPN1 antibody(TA503037),(Red), compared to a nonspecific negative control antibody,(Blue).
Anti-PTPN1 mouse monoclonal antibody (TA503038) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PTPN1(RC204902).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PTPN1 (RC204902, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PTPN1.
HEK293T cells transfected with either RC204902 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-PTPN1 antibody(TA503038), and then analyzed by flow cytometry.
Flow cytometric Analysis of Jurkat cells, using anti-PTPN1 antibody(TA503038),(Red), compared to a nonspecific negative control antibody,(Blue).
Flow cytometric Analysis of Hela cells, using anti-PTPN1 antibody(TA503038),(Red), compared to a nonspecific negative control antibody,(Blue).
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-PTPN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503188)
Anti-PTPN1 mouse monoclonal antibody (TA503188) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PTPN1(RC204902).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PTPN1 (RC204902, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PTPN1.
HEK293T cells transfected with either RC204902 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-PTPN1 antibody(TA503188), and then analyzed by flow cytometry.
Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-PTPN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503309)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-PTPN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503309)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PTPN1 (RC204902, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PTPN1.
Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-PTPN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503310)
Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-PTPN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503310)
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-PTPN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503310)
Anti-PTPN1 mouse monoclonal antibody (TA503310) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PTPN1(RC204902).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PTPN1 (RC204902, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PTPN1.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PTPN1 monoclonal antibody.
HEK293T cells transfected with either RC204902 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-PTPN1 antibody(TA503310), and then analyzed by flow cytometry.
Immunohistochemical staining of paraffin-embedded Human liver tissue using anti-PTPN1 Rabbit Polyclonal antibody. (TA590672)
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-PTPN1 Rabbit Polyclonal antibody. (TA590672)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue using anti-PTPN1 Rabbit Polyclonal antibody. (TA590672)
Immunohistochemical staining of paraffin-embedded Human lymph node tissue using anti-PTPN1 Rabbit Polyclonal antibody. (TA590672)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PTPN1 (RC204902, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PTPN1.
Western Blot: - Samples: Lane 1, Marker: 250, 130, 95, 72, 55, 36, 28, 17, 11 Lane 2, RT-4 Lane 3, U-251MG sp Lane 4, Human Plasma Lane 5, Liver Lane 6, Tonsil, Target weight 50 kDa.
Immunohistochemical analysis of PTP1B (pS50) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugad compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Western blot analysis of PTP1B (pS50) expression in COLO205 (A); human muscle (B); mouse heart (C) whole cell lysates.
Immunohistochemical analysis of PTP1B staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugad compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of PTP1B staining in SKNSH cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Western blot analysis of PTP1B expression in HeLa (A); Jurkat (B) whole cell lysates.
Western blot analysis of PTP1B expression in UV treated COS7 whole cell lysates,The lane on the left is treated with the antigen-specific peptide.
Western blot analysis of PTP1B phosphorylation expression in UV treated COS7 whole cell lysates,The lane on the left is treated with the antigen-specific peptide.
- Quick-Filters:
- Highly Validated (5)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA503037 | PTPN1 antibody |
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| PTPN1 (PTP1B) mouse monoclonal antibody, clone OTI1B4 (formerly 1B4) | |||||||
 |
Mouse | IgG2a | OTI1B4 | Purified | Hu | F, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
 +3 additional images |
Highly validated antibody!
|
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| TA503038 | PTPN1 antibody |
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| PTPN1 (PTP1B) mouse monoclonal antibody, clone OTI1A2 (formerly 1A2) | |||||||
 |
Mouse | IgG2a | OTI1A2 | Purified | Hu | F, ICC/IF, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +4 additional images |
Highly validated antibody!
|
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| TA503188 | PTPN1 antibody |
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| PTPN1 (PTP1B) mouse monoclonal antibody, clone OTI2G3 (formerly 2G3) | |||||||
 |
Mouse | IgG2a | OTI2G3 | Purified | Hu | F, ICC/IF, P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +3 additional images |
Highly validated antibody!
|
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| TA503310 | PTPN1 antibody |
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| PTPN1 (PTP1B) mouse monoclonal antibody, clone OTI1D10 (formerly 1D10) | |||||||
 |
Mouse | IgG2a | OTI1D10 | Purified | Can, Hu, Mky | F, ICC/IF, P, WB |
0.1 ml /
€379.00
|
| OriGene Technologies, Inc. | |||||||
| +6 additional images |
Highly validated antibody!
|
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| TA590672 | PTPN1 antibody |
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| Rabbit Polyclonal PTPN1 Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu | P, WB |
0.1 mg /
€409.00
|
|
| OriGene Technologies, Inc. | |||||||
| +5 additional images |
Highly validated antibody! |
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| TA327798 | PTPN1 antibody |
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| Rabbit Polyclonal Phospho-PTP1B (Ser50) Antibody (Phospho-specific) | |||||||
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Rabbit | IgG | Purified | Hu, Ms, Rt | WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA327856 | PTPN1 antibody |
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| Rabbit Polyclonal PTP1B Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA503309 | PTPN1 antibody |
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| PTPN1 (PTP1B) mouse monoclonal antibody, clone OTI1C5 (formerly 1C5) | |||||||
 |
Mouse | IgG2a | OTI1C5 | Purified | Hu | P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
|
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| AP06575PU-N | PTPN1 antibody |
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 |
Rabbit | Aff - Purified | Hu, Ms | P, WB |
0.1 mg /
€370.00
|
||
| OriGene Technologies GmbH | |||||||
| +1 additional image |
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| AP15219PU-N | PTPN1 antibody |
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Rabbit | Ig | Purified | Hu, Ms | WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies GmbH | |||||||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP304902 | PTPN1 |
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| Recombinant protein of human protein tyrosine phosphatase, non-receptor type 1 (PTPN1) | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
| TP710202 | PTPN1 (full length, C-term DDK tag) |
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| Purified recombinant protein of Human protein tyrosine phosphatase, non-receptor type 1 (PTPN1), full length, with C-terminal DDK tag, expressed in sf9, 20ug | |||||
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Human | > 80 % Preparation: . Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
SF9 cells |
20 µg /
€411.00
|
|
| OriGene Technologies, Inc. | |||||
| TP750046 | PTPN1 |
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| Recombinant protein of human protein tyrosine phosphatase, non-receptor type 1 (PTPN1) produced in E. coli. | |||||
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Human | > 90 % Preparation: . Purity Detail: > 90% as determined by SDS-PAGE and Coomassie blue staining. |
E. coli |
0.1 mg /
€411.00
|
|
| OriGene Technologies, Inc. | |||||
| SA6014 | PTPN1 (1-321) |
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 |
Human | Purified | > 95 % by SDS-PAGE | E. coli |
0.1 mg /
€250.00
|
| OriGene Technologies GmbH | |||||
| +1 additional image |
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| SA6014X | PTPN1 (1-321) |
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|
Human | Purified | > 95 % by SDS-PAGE | E. coli |
0.5 mg /
€600.00
|
| OriGene Technologies GmbH | |||||
| +1 additional image |
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Rat Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RR205311L2V | Lenti ORF particles, Ptpn1 (GFP-tagged ORF) - Rat protein tyrosine phosphatase, non-receptor type 1 (Ptpn1), 200 uL, >10^7 TU/mL |
|
 |
200 µl /
€930.00
|
|
| OriGene Technologies, Inc. | ||
| RR205311L1V | Lenti ORF particles, Ptpn1 (Myc-DDK-tagged ORF) - Rat protein tyrosine phosphatase, non-receptor type 1 (Ptpn1), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€830.00
|
|
| OriGene Technologies, Inc. | ||
