PTPN20 antibody
Principal name
PTPN20 antibody
Alternative names for PTPN20 antibody
PTPN20A, PTPN20B, RP11-142I17.1, Tyrosine-protein phosphatase non-receptor type 20
Gene ID
105369264 (LOC105369264), 653129 (PTPN20A), 26095 (PTPN20B), 19256 (Ptpn20), 306281 (Ptpn20b)
Ncbi ID
NP_001035816, AAI41461.1, NM_001042358, NP_001035822, NP_001035816.1, NP_033004.1, NM_008978, NM_001080149, NM_001042397, NM_015605, NM_001042392, NM_001042361, NM_001042357, NM_001042391, NM_001042389, NM_001042359, NM_001042394, NM_001042393, NM_001042395, NM_001042390, NM_001042387, NM_001042365
Available reactivities
Available hosts
Available applications
Paraffin Sections (P), Flow Cytometry (F), Western blot / Immunoblot (WB), Enzyme Immunoassay (E)
Background of PTPN20 antibody
- Kit Component:
- - KN314213G1, Ptpn20 gRNA vector 1 in pCas-Guide vector
- KN314213G2, Ptpn20 gRNA vector 2 in pCas-Guide vector
- KN314213D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Matsuoka, S., et al. Science 316(5828): 1160-1166 (2007)
Fodero-Tavoletti, M.T., et al. Biochem. J. 389 (PT 2): 343-354 (2005)
Immunohistochemistry analysis in human stomach tissue (Formalin-fixed, Paraffin-embedded) using PTPN20 antibody Cat.-No. AP53510PU-N, followed by peroxidase conjugated secondary antibody and DAB staining.This data demonstrates the use of PTPN20 antibody for IHC. Clinical relevance has not been evaluated.
Western blot analysis in K562 cell line lysates (35ug/lane) using PTPN20 antibody Cat.-No. AP53510PU-N. This demonstrates the PTPN20A antibody detected the PTPN20A protein (arrow).
Flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram) using PTPN20 antibody Cat.-No. AP53510PU-N, followed by FITC-conjugated goat-anti-rabbit secondary antibodies.
Host: Rabbit; Target Name: PTPN20B; Sample Tissue: THP-1 Whole Cell lysates; Antibody Dilution: 1.0ug/ml
Host: Rabbit; Target Name: PTPN20A; Sample Tissue: MCF7 Whole Cell lysates; Antibody Dilution: 1.0 ug/ml
Gel: 8%SDS-PAGE, Lysate: 40 ug, Lane 1-2: K562 cells, human testis tissue, Primary antibody: (PTPN20B Antibody) at dilution 1/200, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 minute
The image on the left is immunohistochemistry of paraffin-embedded Human cervical cancer tissue using (PTPN20B Antibody) at dilution 1/30, on the right is treated with synthetic peptide. (Original magnification: ×200)
The image on the left is immunohistochemistry of paraffin-embedded Human ovarian cancer tissue using (PTPN20B Antibody) at dilution 1/30, on the right is treated with synthetic peptide. (Original magnification: ×200)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA351565 | PTPN20 antibody |
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| Rabbit Polyclonal Anti-PTPN20B Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu | E, P, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
 +2 additional images |
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| AP53510PU-N | PTPN20 (Center) antibody |
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 |
Rabbit | Ig | Aff - Purified | Hu | F, P, WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies GmbH | |||||||
| +2 additional images |
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Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP312878 | PTPN20 (transcript variant 8) |
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| Purified recombinant protein of Homo sapiens protein tyrosine phosphatase, non-receptor type 20B (PTPN20B), transcript variant 8 | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY420847 | PTPN20 overexpression lysate |
|
 |
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. |
Rat Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RR213028L2V | Lenti ORF particles, Ptpn20b (GFP-tagged ORF) - Rat protein tyrosine phosphatase, non-receptor type 20 (Ptpn20), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€930.00
|
|
| OriGene Technologies, Inc. | ||
| RR213028L1V | Lenti ORF particles, Ptpn20b (Myc-DDK-tagged ORF) - Rat protein tyrosine phosphatase, non-receptor type 20 (Ptpn20), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€830.00
|
|
| OriGene Technologies, Inc. | ||