PURB antibody
Principal name
PURB antibody
Alternative names for PURB antibody
Pur-beta, Transcriptional activator protein Pur-beta, Purine-rich element-binding protein B
Ncbi ID
NP_150093, NM_033224, NP_150093.1, NP_035351.1, NM_011221, NM_001017503
Available reactivities
Available hosts
Available applications
Western blot / Immunoblot (WB), Flow Cytometry (F)
Background of PURB antibody
- Kit Component:
- - KN314257G1, Purb gRNA vector 1 in pCas-Guide vector
- KN314257G2, Purb gRNA vector 2 in pCas-Guide vector
- KN314257D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Joslyn, G., et al. Alcohol. Clin. Exp. Res. 34(5):800-812 (2010).
Ramsey, J.E., et al. Biochemistry 48(27):6348-6360 (2009).
Sugiyama, N., et al. Mol. Cell Proteomics 6(6):1103-1109 (2007).
Olsen, J.V., et al. Cell 127(3):635-648 (2006).
Olsen, J.V., et al. Cell 127(3):635-648 (2006).
Human THP-1; WB Suggested Anti-PURB Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: THP-1 cell lysate; PURB antibody - N-terminal region (ARP41073_P050) in Human THP-1 cells using Western Blot
PURB Antibody (C-term) (Cat. #TA324468) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the PURB antibody detected the PURB protein (arrow).
IF image of A549 cell stained with PURB Antibody (C-term)(Cat#TA324468).A549 cells were incubated with PURB primary antibody (1:25, 1 h at 37?). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7 units/ml).PURB immunoreactivity is localized to Nucleus significantly.
PURB Antibody (C-term) (Cat. #TA324468) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
WB Suggested Anti-PURB Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 62500; Positive Control: THP-1 cell lysate
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA324468 | PURB (C-term) antibody |
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| Rabbit polyclonal PURB Antibody (C-term) | |||||||
 |
Rabbit | Ig | Aff - Purified | Hu | F, WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies, Inc. | |||||||
 +2 additional images |
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Lysates
| Catalog No. | ||
|---|---|---|
| LY409642 | PURB overexpression lysate |
|
 |
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. |
Rat Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RR204335L2V | Lenti ORF particles, Purb (GFP-tagged ORF) - Rat purine rich element binding protein B (Purb), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€930.00
|
|
| OriGene Technologies, Inc. | ||
| RR204335L1V | Lenti ORF particles, Purb (Myc-DDK-tagged ORF) - Rat purine rich element binding protein B (Purb), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€830.00
|
|
| OriGene Technologies, Inc. | ||
