PUS1 antibody
Principal name
PUS1 antibody
Alternative names for PUS1 antibody
PP8985, Pseudouridylate synthase 1, tRNA-uridine isomerase I, MLASA1, MGC11268, tRNA pseudouridine synthase A mitochondrial, tRNA pseudouridine(38-40) synthase, tRNA pseudouridylate synthase I
Ncbi ID
9606, NP_001020733, NM_001025562, NP_001002019, NP_001002019.1, NP_079491, NP_001002020, NP_001020733.1, NM_019700, NM_001025561, NM_001025563
Available reactivities
Available hosts
Available applications
Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Enzyme Immunoassay (E), Paraffin Sections (P), Immunoprecipitation (IP)
Background of PUS1 antibody
- Kit Component:
- - KN314259G1, Pus1 gRNA vector 1 in pCas-Guide vector
- KN314259G2, Pus1 gRNA vector 2 in pCas-Guide vector
- KN314259D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Bergmann, A.K., et al. Pediatr Blood Cancer 54(2):273-278 (2010).
Sibert, B.S., et al. RNA 14(9):1895-1906 (2008).
Rikova, K., et al. Cell 131(6):1190-1203 (2007).
Sugiyama, N., et al. Mol. Cell Proteomics 6(6):1103-1109 (2007).
Fernandez-Vizarra, E., et al. J. Med. Genet. 44(3):173-180 (2007).
Mouse SP2/0; WB Suggested Anti-PUS1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: SP2/0 cell lysate; PUS1 antibody - C-terminal region (ARP37261_P050) in Mouse SP2/0 cells using Western Blot
Western Blot analysis of PUS1 expression in transfected 293T cell line (H00080324-T01) by PUS1 MaxPab polyclonal antibody.Lane 1: PUS1 transfected lysate(44 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of PUS1 expression in transfected 293T cell line (H00080324-T02) by PUS1 MaxPab polyclonal antibody.Lane 1: PUS1 transfected lysate(43.89 KDa).Lane 2: Non-transfected lysate.
Mouse Kidney; WB Suggested Anti-Pus1 Antibody Titration: 0.2-1 ug/ml. Positive Control: Mouse Kidney; Pus1 antibody - N-terminal region (ARP37260_P050) in Mouse Kidney cells using Western Blot
Formalin fixed, paraffin embedded human skeletal muscle stained with PUS1 Antibody (C-term) Cat.-No AP53521PU-N followed by peroxidase conjugation of the secondary antibody and DAB staining
Western blot analysis of PUS1 Antibody (C-term) Cat.-No AP53521PU-N in NCI-H460 cell line lysates (35ug/lane). This demonstrates the PUS1 antibody detected the PUS1 protein (arrow).
WB Suggested Anti-Pus1 Antibody Titration: 0.2-1 ug/ml; Positive Control: Mouse Kidney
WB Suggested Anti-PUS1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: SP2/0 cell lysate
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| AP53521PU-N | PUS1 (C-term) antibody |
||||||
 |
Rabbit | Ig | Aff - Purified | Hu | P, WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies GmbH | |||||||
 +1 additional image |
|||||||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP300879 | PUS1 (transcript variant 1) |
||||
| Recombinant protein of human pseudouridylate synthase 1 (PUS1), transcript variant 1 | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
| TP302637 | PUS1 (transcript variant 2) |
||||
| Recombinant protein of human pseudouridylate synthase 1 (PUS1), transcript variant 2 | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
| TP322753 | PUS1 (transcript variant 3) |
||||
| Recombinant protein of human pseudouridylate synthase 1 (PUS1), transcript variant 3 | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR206726L2V | Lenti ORF particles, Pus1 (GFP-tagged) - Mouse pseudouridine synthase 1 (Pus1), transcript variant 3, 200 uL, >10^7 TU/mL |
|
 |
200 µl /
€930.00
|
|
| OriGene Technologies, Inc. | ||
| MR206726L1V | Lenti ORF particles, Pus1 (Myc-DDK-tagged) - Mouse pseudouridine synthase 1 (Pus1), transcript variant 3, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€830.00
|
|
| OriGene Technologies, Inc. | ||
Rat Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RR204099L2V | Lenti ORF particles, Pus1 (GFP-tagged ORF) - Rat pseudouridylate synthase 1 (Pus1), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€930.00
|
|
| OriGene Technologies, Inc. | ||
| RR204099L1V | Lenti ORF particles, Pus1 (Myc-DDK-tagged ORF) - Rat pseudouridylate synthase 1 (Pus1), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€830.00
|
|
| OriGene Technologies, Inc. | ||