PUS10 antibody
Principal name
PUS10 antibody
Alternative names for PUS10 antibody
CCDC139, DOBI, Pseudouridylate synthase 10, tRNA-uridine isomerase, Psi55 synthase, FLJ3231, Putative tRNA pseudouridine synthase Pus10, tRNA pseudouridylate synthase
Ncbi ID
Available reactivities
Available hosts
Available applications
Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P)
Background of PUS10 antibody
Pseudouridition, the isomerization of uridine to pseudouridine, is the most common posttranscriptiol nucleotide modification found in R and is essential for biologic functions such as spliceosome biogenesis. Pseudouridylate synthases, such as PUS10, catalyze pseudouridition of structural Rs, including transfer, ribosomal, and splicing Rs. These enzymes also act as R chaperones, facilitating the correct folding and assembly of tRs (McCleverty et al., 2007 [PubMed 17900615]).
Human HCT15; WB Suggested Anti-PUS10 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: HCT15 cell lysate; PUS10 antibody - middle region (ARP52750_P050) in Human HCT15 cells using Western Blot
WB Suggested Anti-PUS10 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 312500; Positive Control: HCT15 cell lysate
Gel: 8%SDS-PAGE, Lysate: 40 ug, Lane: Mouse intestinum tenue tissue, Primary antibody: (PUS10 Antibody) at dilution 1/200, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 3 seconds
The image on the left is immunohistochemistry of paraffin-embedded Human breast cancer tissue using (PUS10 Antibody) at dilution 1/45, on the right is treated with synthetic peptide. (Original magnification: ×200)
The image on the left is immunohistochemistry of paraffin-embedded Human brain tissue using (PUS10 Antibody) at dilution 1/45, on the right is treated with synthetic peptide. (Original magnification: ×200)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA351567 | PUS10 antibody |
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| Rabbit Polyclonal Anti-PUS10 Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms | E, P, WB |
0.1 ml /
€335.00
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|
| OriGene Technologies, Inc. | |||||||
 +2 additional images |
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Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP310979 | PUS10 |
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| Recombinant protein of human pseudouridylate synthase 10 (PUS10) | |||||
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Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY408160 | PUS10 overexpression lysate |
|
 |
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. |
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR208455L2V | Lenti ORF particles, Pus10 (GFP-tagged) - Mouse pseudouridylate synthase 10 (Pus10), transcript variant 3, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,220.00
|
|
| OriGene Technologies, Inc. | ||
| MR208455L1V | Lenti ORF particles, Pus10 (Myc-DDK-tagged) - Mouse pseudouridylate synthase 10 (Pus10), transcript variant 3, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,090.00
|
|
| OriGene Technologies, Inc. | ||