PYGO1 antibody
Principal name
PYGO1 antibody
Alternative names for PYGO1 antibody
Pygopus homolog 1
Ncbi ID
9606, NP_056432, NM_015617, NP_056432.1, NP_082392.1, NM_028116
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P)
Background of PYGO1 antibody
- Kit Component:
- - KN314292G1, Pygo1 gRNA vector 1 in pCas-Guide vector
- KN314292G2, Pygo1 gRNA vector 2 in pCas-Guide vector
- KN314292D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Frank, B., et al. Carcinogenesis (2010) In press.
Fiedler, M., et al. Mol. Cell 30(4):507-518 (2008).
Townsley, F.M., et al. J. Biol. Chem. 279(7):5177-5183 (2004).
Thompson, B., et al. Nat. Cell Biol. 4(5):367-373 (2002).
Kramps, T., et al. Cell 109(1):47-60 (2002).
Western Blot analysis of PYGO1 expression in transfected 293T cell line (H00026108-T01) by PYGO1 MaxPab polyclonal antibody.Lane 1: PYGO1 transfected lysate(46.09 KDa).Lane 2: Non-transfected lysate.
Human Brain; WB Suggested Anti-PYGO1 Antibody Titration: 0.2-1 ug/ml. Positive Control: Human brain; PYGO1 antibody - N-terminal region (ARP50601_P050) in Human Brain cells using Western Blot
Human NCI-H226; WB Suggested Anti-PYGO1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: NCI-H226 cell lysate.. PYGO1 is strongly supported by BioGPS gene expression data to be expressed in NCI-H226.; PYGO1 antibody - middle region (ARP50602_P050) in Human NCI-H226 cells using Western Blot
PYGO1 Antibody (C-term) (Cat. #TA324480) western blot analysis in ZR-75-1 cell line lysates (35ug/lane).This demonstrates the PYGO1 antibody detected the PYGO1 protein (arrow).
PYGO1 Antibody (C-term) (Cat. #TA324480)immunohistochemistry analysis in formalin fixed and paraffin embedded human placenta tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PYGO1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Confocal immunofluorescent analysis of PYGO1 Antibody (C-term)(Cat#TA324480) with ZR-75-1 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).
WB Suggested Anti-PYGO1 Antibody Titration: 0.2-1 ug/ml; Positive Control: Human brain
WB Suggested Anti-PYGO1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 62500; Positive Control: NCI-H226 cell lysate.PYGO1 is strongly supported by BioGPS gene expression data to be expressed in NCI-H226
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA324480 | PYGO1 (C-term) antibody |
||||||
| Rabbit polyclonal PYGO1 Antibody (C-term) | |||||||
 |
Rabbit | Ig | Aff - Purified | Hu | ICC/IF, P, WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies, Inc. | |||||||
 +2 additional images |
|||||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY414458 | PYGO1 overexpression lysate |
|
 |
0.1 mg /
€495.00
|
|
| OriGene Technologies, Inc. |