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RAC2 antibody

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Principal name

RAC2 antibody

Alternative names for RAC2 antibody

p21-Rac2, Ras-related C3 botulinum toxin substrate 2, Small G protein

SwissProt ID

O88931 (Cavpo), P15153 (Human), Q05144 (Mouse), Q9TU25 (Bovin)

Gene ID

5880 (RAC2), 19354 (Rac2)

Ncbi ID

NP_002863, 9606, NP_002863.1, NP_033034.1, NM_002872, NM_009008

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat)

Available hosts

Mouse, Goat, Rabbit

Available applications

Flow Cytometry (F), Immunocytochemistry/Immunofluorescence (ICC/IF), Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Frozen Sections (C)

Background of RAC2 antibody

Rac and Cdc42 are members of the Rho-GTPase family. In mammals, Rac exists as three isoforms, Rac1, Rac2 and Rac3, which are highly similar in sequence. Rac1 and Cdc42, the most widely studied of this group, are ubiquitously expressed. Rac2 is expressed in cells of hematopoietic origin, and Rac3, while highly expressed in brain, is also found in many other tissues. Rac and Cdc42 play key sigling roles in cytoskeletal reorganization, membrane trafficking, transcriptiol regulation, cell growth and development. GTP binding stimulates the activity of Rac/Cdc42, and the hydrolysis of GTP to GDP through the protein's intrinsic GTPase activity, rendering it ictive. GTP hydrolysis is aided by GTPase activating proteins (GAPs), while exchange of GDP for GTP is facilitated by guanine nucleotide exchange factors (GEFs). Another level of regulation is achieved through the binding of RhoGDI, a guanine nucleotide dissociation inhibitor, which retains Rho family GTPases, including Rac and Cdc42, in their ictive GDP-bound state.

General readings

Reibel L, Dorseuil O, Stancou R, Bertoglio J, Gacon G.
A hemopoietic specific gene encoding a small GTP binding protein is overexpressed during T cell activation.
Biochem Biophys Res Commun. 1991 Mar 15;175(2):451-8.
PMID: 1902092

AM39043PU-N - RAC2
ICC/IF analysis of RAC2 in Jurkat cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti human RAC2 antibody (1: 100) with goat anti mouse IgG - Alexa fluor 488 conjugate (Green).
AM39043PU-N - RAC2
Flow Cytometry analysis of RAC2 in Jurkat cell line, staining at 2-5ug for 1x106 cells (red line). The secondary antibody used goat anti mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).
AM39043PU-N - RAC2
Western blot analysis:The extracts of Jurkat were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human RAC2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
AR09246PU-L - RAC2
AR09246PU-L - RAC2
Recombinant human RAC2, 1-189 aa, His-tagged: 15% SDS-PAGE (3 µg)
H00005880-M08 - RAC2
Western Blot analysis of RAC2 expression in transfected 293T cell line by RAC2 monoclonal antibody (M08), clone 3B8.Lane 1: RAC2 transfected lysate(21.4 KDa).Lane 2: Non-transfected lysate.
PAB6111 - RAC2
RAC2 polyclonal antibody ( Cat # PAB6111 ) staining ( 0.3 µg/ml ) of paraffin embedded human tonsil. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.A) Strong staining in the interfollicular area and mantle zone.B) Positive cells in the interfollicular area.
PAB6111 - RAC2
RAC2 polyclonal antibody ( Cat # PAB6111 ) staining ( 0.3 µg/ml ) of human tonsil lysate ( RIPA buffer, 35 µg total protein per lane ). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
CPA3002-100ul - RAC2
Western blot analysis of RAC2 expression in Jurkat (A); SP2/0 (B); H9C2 (C) whole cell lysates.
TA322940 - RAC2
Predicted band size: 21 kDa . Positive control: Jurkat and HL-60 cell lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lane1: Jurkat cell lysate Lane2: HL-60 cell lysate Lysates: 40 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute)
TA337190 - RAC2
Western Blot: RAC2 Antibody [TA337190] - Analysis of RAC2 in Human Tonsil lysate (RIPA buffer, 35ug total protein per lane) using at 0.3ug/ml. Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
TA337190 - RAC2
Immunohistochemistry-Paraffin: RAC2 Antibody [TA337190] - Staining of human tonsil using this antibody. A) Strong staining in the interfollicular area and mantle zone but the majority of cells in germinal centre are negative. B) Higher magnification of in
TA302763 - RAC2
TA302763 staining (2µg/ml) of MOLT-4 lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
TA302763 - RAC2
TA302763retrieval with Tris/EDTA buffer pH9, HRP-staining. A) Strong staining in the interfollicular area and mantle zone. B) Positive cells in the interfollicular area.

6 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

RAC2 (C-term) antibody
  Goat Polyclonal Antibody against RAC2  
TA302763 staining (2µg/ml) of MOLT-4 lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence. Goat Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €360.00
  OriGene Technologies, Inc.
+1 additional image

RAC2 antibody
  Anti-RAC2 Rabbit Polyclonal Antibody  
Predicted band size: 21 kDa . Positive control: Jurkat and HL-60 cell lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lane1: Jurkat cell lysate Lane2: HL-60 cell lysate Lysates: 40 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €335.00
  OriGene Technologies, Inc.

RAC2 (1-189) antibody
ICC/IF analysis of RAC2 in Jurkat cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti human RAC2 antibody (1: 100) with goat anti mouse IgG - Alexa fluor 488 conjugate (Green). Mouse IgG2b AT2G11 Purified Hu E, F, ICC/IF, WB
0.1 ml / €330.00
  OriGene Technologies GmbH
+2 additional images

RAC2 (1-189) antibody
ICC/IF analysis of RAC2 in Jurkat cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti human RAC2 antibody (1: 100) with goat anti mouse IgG - Alexa fluor 488 conjugate (Green). Mouse IgG2b AT2G11 Purified Hu E, F, ICC/IF, WB
50 µl / €220.00
  OriGene Technologies GmbH
+2 additional images

Mouse Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Rac2 (GFP-tagged) - Mouse RAS-related C3 botulinum substrate 2 (Rac2), 200 uL, >10^7 TU/mL
Lenti ORF particles, Rac2 (GFP-tagged) - Mouse RAS-related C3 botulinum substrate 2 (Rac2), 200 uL, >10^7 TU/mL  
200 µl / €930.00
  OriGene Technologies, Inc.
 

Lenti ORF particles, Rac2 (Myc-DDK-tagged) - Mouse RAS-related C3 botulinum substrate 2 (Rac2), 200 uL, >10^7 TU/mL
Lenti ORF particles, Rac2 (Myc-DDK-tagged) - Mouse RAS-related C3 botulinum substrate 2 (Rac2), 200 uL, >10^7 TU/mL  
200 µl / €830.00
  OriGene Technologies, Inc.
 

6 Item(s)

per page

Available Filters

Product Type
  1. Mouse Lenti ORF Particle (2)
  2. Primary Antibody (4)
Clonality
  1. Monoclonal (2)
  2. Polyclonal (2)
Target
  1. RAC2 (4)
Property
  1. 1-189 (2)
  2. C-term (1)
Reactivity
  1. Human (Hu) (4)
  2. Mouse (Ms) (2)
  3. Rat (Rt) (2)
Applications
  1. Western blot / Immunoblot (WB) (4)
  2. Enzyme Immunoassay (E) (2)
  3. Flow cytometry (F) (2)
  4. Immunocytochemistry/​Immunofluorescence (ICC/IF) (2)
  5. Paraffin Sections (P) (1)
Presentation
  1. Purified (3)
  2. Aff - Purified (1)
Host
  1. Mouse (2)
  2. Goat (1)
  3. Rabbit (1)
Images
  1. 1 image (4)
  2. 3+ images (2)
Images: Applications
  1. Western blot / Immunoblot (WB) (4)
  2. Flow cytometry (F) (2)
  3. Immunocytochemistry/​Immunofluorescence (ICC/IF) (2)
  4. IHC (1)
Manufacturer
  1. OriGene Technologies (6)
Service
  • OriGene Technologies GmbH
  • Schillerstraße 5
  • D-32052 Herford
  • Germany
  • phone: +49-5221-34606-0
  • free call: 0800-227 47 46 (Germany only)
  • fax: +49-5221-34606-11
  • email: info-de@origene.com
  • orders: ordering-de@origene.com
 
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