RAD1 antibody
Principal name
RAD1 antibody
Alternative names for RAD1 antibody
REC1, Cell cycle checkpoint protein RAD1, DNA repair exonuclease rad1 homolog, Rad1-like DNA damage checkpoint protein
Ncbi ID
NP_002844.1, 9606, NP_002844, NM_002853, AAH06837, NP_035362.2, NM_133377
Available reactivities
Available hosts
Available applications
Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), ELISA (detection) (E(detection))
Background of RAD1 antibody
- Kit Component:
- - KN314406G1, Rad1 gRNA vector 1 in pCas-Guide vector
- KN314406G2, Rad1 gRNA vector 2 in pCas-Guide vector
- KN314406D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Liu Y., et al. (2010) J Biol Chem. 285(8):5974-82.
Yazinski SA., et al. (2009) Proc Natl Acad Sci U S A. 106(50):21282-7.
Testis: Formalin-Fixed Paraffin-Embedded (FFPE)
The RAD1 polyclonal antibody ( Cat # PAB0166 ) was diluted at 2 µg/ml ( lane 1 ) and 1 µg/ml ( lane 2 ) and tested against 10 ug of 293 cell lysate by western blotting. A single band at 66 kDa is detected.
Immunoperoxidase of monoclonal antibody to RAD1 on formalin-fixed paraffin-embedded human lateral ventricle wall. [antibody concentration 3 ug/ml]
Western Blot analysis of RAD1 expression in transfected 293T cell line by RAD1 monoclonal antibody (M01), clone 1G2.Lane 1: RAD1 transfected lysate(32.43 KDa).Lane 2: Non-transfected lysate.
Western blot analysis of RAD1 over-expressed 293 cell line, cotransfected with RAD1 Validated Chimera RNAi ( Cat # H00005810-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with RAD1 monoclonal antibody (M01), clone 1G2 (Cat # H00005810-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Detection limit for recombinant GST tagged RAD1 is approximately 0.3ng/ml as a capture antibody.
Detection limit for recombinant GST tagged RAD1 is approximately 1ng/ml as a capture antibody.
Immunofluorescence of purified MaxPab antibody to RAD1 on HeLa cell. [antibody concentration 10 ug/ml]
Western Blot analysis of RAD1 expression in transfected 293T cell line (H00005810-T01) by RAD1 MaxPab polyclonal antibody.Lane 1: RAD1 transfected lysate(31.02 KDa).Lane 2: Non-transfected lysate.
Recombinant human RAD1, 1-282 aa, His-tagged
Human COLO205; WB Suggested Anti-RAD1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: COLO205 cell lysate. RAD1 is strongly supported by BioGPS gene expression data to be expressed in Human COLO205 cells.; RAD1 antibody - middle region (ARP57717_P050) in Human COLO205 cells using Western Blot
Western blot analysis of RAD1 Antibody Cat.-No AP53557PU-N in K562 cell line lysates (35ug/lane). This demonstrates the RAD1 antibody detected the RAD1 protein (arrow).
Western blot analysis of RAD1 expression in A549 (A); Raw264.7 (B); H9C2 (C) whole cell lysates.
WB Suggested Anti-RAD1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:1562500; Positive Control: COLO205 cell lysateRAD1 is strongly supported by BioGPS gene expression data to be expressed in Human COLO205 cells
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA308831 | RAD1 antibody |
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| Rabbit polyclonal antibody to RAD1 (RAD1 homolog (S. pombe)) | |||||||
 |
Rabbit | IgG | Aff - Purified | Hu, Ms | P, WB |
0.1 ml /
€240.00
|
|
| OriGene Technologies, Inc. | |||||||
| AM06054PU-N | RAD1 (N-term) antibody |
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 |
Mouse | IgG | 4126 | Purified | Hu | P, WB |
50 µl /
€425.00
|
| OriGene Technologies GmbH | |||||||
| AP53557PU-N | RAD1 (Center) antibody |
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 |
Rabbit | Ig | Aff - Purified | Hu | WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies GmbH | |||||||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP316420 | RAD1 (transcript variant 1) |
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| Recombinant protein of human RAD1 homolog (S. pombe) (RAD1), transcript variant 1 | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
