RAD51C antibody
Principal name
RAD51C antibody
Alternative names for RAD51C antibody
RAD51L2, R51H3, RAD51 homolog C, RAD51-like protein 2, DNA repair protein RAD51 homolog 3
Gene ID
5889 (RAD51C)
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Paraffin Sections (P), Immunoprecipitation (IP)
Background of RAD51C antibody
- Kit Component:
- - KN210462G1, RAD51C gRNA vector 1 in pCas-Guide vector
- KN210462G2, RAD51C gRNA vector 2 in pCas-Guide vector
- KN210462D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Dosanjh MK, Collins DW, Fan W, Lennon GG, Albala JS, Shen Z, Schild D.
Isolation and characterization of RAD51C, a new human member of the RAD51 family of related genes.
Nucleic Acids Res. 1998 Mar 1;26(5):1179-84.
PMID: 9469824
Immunofluorescence of monoclonal antibody to RAD51C on HeLa cell. [antibody concentration 10 ug/ml]
Western blot analysis of RAD51C over-expressed 293 cell line, cotransfected with RAD51C Validated Chimera RNAi ( Cat # H00005889-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with RAD51C monoclonal antibody (M01), clone 3F3-5C6 (Cat # H00005889-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Western Blot analysis of RAD51C expression in transfected 293T cell line by RAD51C monoclonal antibody (M01), clone 3F3-5C6.Lane 1: RAD51C transfected lysate(42.2 KDa).Lane 2: Non-transfected lysate.
RAD51C monoclonal antibody (M01), clone 3F3-5C6 Western Blot analysis of RAD51C expression in HeLa ( Cat # L013V1 ).
RAD51C monoclonal antibody (M01), clone 3F3-5C6. Western Blot analysis of RAD51C expression in 293 ( Cat # L026V1 ).
Detection limit for recombinant GST tagged RAD51C is approximately 0.1ng/ml as a capture antibody.
RAD51C polyclonal antibody ( Cat # PAB6271 ) staining ( 2 µg/ml ) of HeLa lysate ( RIPA buffer, 30 µg total protein per lane ) . Primary incubated for 12 hour. Detected by western blot using chemiluminescence.
Western Blot analysis of RAD51C expression in transfected 293T cell line (H00005889-T01) by RAD51C MaxPab polyclonal antibody.Lane 1: RAD51C transfected lysate(41.36 KDa).Lane 2: Non-transfected lysate.
RAD51C detected in HEK293 lysate using RAD51C monoclonal antibody, clone 2H11/6 ( Cat # MAB2387 ). Photo courtesy of B.T. Bennett & K. Knight, University of Massachusetts Medical School.
Western Blot analysis of RAD51C expression in transfected 293T cell line (H00005889-T02) by RAD51C MaxPab polyclonal antibody.Lane 1: RAD51C transfected lysate(42.20 KDa).Lane 2: Non-transfected lysate.
Western blot (WB) analysis of Rad51C antibody (Cat.-No.: AP20492PU-N) in extracts from COS-7 cells.
Immunohistochemical analysis of RAD51C staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugad compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of RAD51C staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Western blot analysis of RAD51C expression in HeLa (A); COS7 (B) whole cell lysates.
TA302837 staining (2µg/ml) of HeLa lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 12 hour. Detected by western blot using chemiluminescence.
Western Blot: Rad51C Antibody (2H11/6) - Rad51C detected in HEK293 lysate using anti-Rad51C. Photo courtesy of B.T. Bennett & K. Knight, University of Massachusetts Medical School.
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA302837 | RAD51C (N-term) antibody |
||||||
| Goat Polyclonal Antibody against RAD51C | |||||||
 |
Goat | Aff - Purified | Hu | WB |
0.1 mg /
€360.00
|
||
| OriGene Technologies, Inc. | |||||||
| TA309826 | RAD51C antibody |
||||||
| Mouse Monoclonal Rad51C Antibody (2H11/6) | |||||||
 |
Mouse | IgG1 | 2H11/6 | Purified | Hu, Ms | WB |
0.1 ml /
€415.00
|
| OriGene Technologies, Inc. | |||||||
| AP20492PU-N | RAD51C antibody |
||||||
 |
Rabbit | Aff - Purified | Hu | ICC/IF, P, WB |
0.1 mg /
€370.00
|
||
| OriGene Technologies GmbH | |||||||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP760472 | RAD51C (full length, N-term HIS tag, transcript variant 2) |
||||
| Purified recombinant protein of Human RAD51 homolog C (S. cerevisiae) (RAD51C), transcript variant 2, full length, with N-terminal HIS tag, expressed in E.Coli, 50ug | |||||
 |
Human | > 80 % Preparation: . Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
E. coli |
50 µg /
€205.00
|
|
| OriGene Technologies, Inc. | |||||