RAD51L1 antibody
Principal name
RAD51L1 antibody
Alternative names for RAD51L1 antibody
R51H2, RAD51B, RAD51 homolog B, RAD51-like protein 1, REC2, DNA repair protein RAD51 homolog 2
SwissProt ID
O15315 (Human), O15315-1, O35719 (Mouse), Q86U92 (Human), Q91917 (Xenla), Q9SK02 (Arath)
Ncbi ID
9606, NP_002868, NM_133510, NP_002868.1, NP_598193, NM_133509, NP_033040.2, BC058184, NM_009014, NM_002877
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Flow Cytometry (F), Immunoprecipitation (IP)
Background of RAD51L1 antibody
- Kit Component:
- - KN314417G1, Rad51b gRNA vector 1 in pCas-Guide vector
- KN314417G2, Rad51b gRNA vector 2 in pCas-Guide vector
- KN314417D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Masson J.Y., Tarsounas M.C. et al. (2001). Genes Dev. 15:3296-3307
Immunohistochemistry analysis of RAD51L1 using pAb Cat.No. AP20559PU-N in Paraffin-embedded human liver carcinoma tissue at 1:50, showing cytoplasmic and nuclear staining. Negative control (the right) using PBS instead of primary antibody, secondary antibody is Goat anti-Rabbit IgG-Biotin followed by avidin-peroxidase.
Immunohistochemistry analysis of RAD51L1 using pAb Cat.No. AP20559PU-N in Paraffin-embedded human lung carcinoma tissue at 1:50, showing cytoplasmic and nuclear staining. Negative control (the right) using PBS instead of primary antibody, secondary antibody is Goat anti-Rabbit IgG-Biotin followed by avidin-peroxidase.
Immunohistochemistry analysis of RAD51L1 using pAb Cat.No. AP20559PU-N in Paraffin-embedded human colon carcinoma tissue at 1:50, showing cytoplasmic and nuclear staining. Negative control (the right) using PBS instead of primary antibody, secondary antibody is Goat anti-Rabbit IgG-Biotin followed by avidin-peroxidase.
Western blot (WB) analysis of Rad51L1 (Rad51B) antibody (Cat.-No.: AP20559PU-N) in extracts from COS-7 cells.
Human HepG2; WB Suggested Anti-RAD51L1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: HepG2 cell lysate; RAD51L1 antibody - middle region (ARP57720_P050) in Human HepG2 cells using Western Blot
Hum. Fetal Brain; Host:Rabbit. Target Name:RAD51B. Sample Tissue:Human Fetal Brain. Antibody Dilution: 1.0ug/ml.; RAD51L1 antibody - middle region (ARP57720_P050) in Hum. Fetal Brain cells using Western Blot
Hum. Adult Placenta; Host:Rabbit. Target Name:RAD51B. Sample Tissue:Human Adult Placenta. Antibody Dilution: 1.0ug/ml.; RAD51L1 antibody - middle region (ARP57720_P050) in Hum. Adult Placenta cells using Western Blot
Human COLO205; WB Suggested Anti-RAD51L1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: COLO205 cell lysate. RAD51B is supported by BioGPS gene expression data to be expressed in COLO205.; RAD51L1 antibody - middle region (ARP57721_P050) in Human COLO205 cells using Western Blot
Anti-RAD51L1 mouse monoclonal antibody (TA503550) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY RAD51L1(RC206457).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RAD51L1 (RC206457, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RAD51L1.
Western blot analysis of extracts (10ug) from 2 different cell lines by using anti-RAD51L1 monoclonal antibody at 1:200 dilution.
HEK293T cells transfected with either RC206457 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-RAD51L1 antibody(TA503550), and then analyzed by flow cytometry.
Western blot analysis of extracts from COS-7 cells, using RAD51L1 antibody.The lane on the right is treated with the synthesized peptide.
Immunohistochemistry analysis of paraffin-embedded human pancreas tissue using RAD51L1 antibody.The picture on the right is treated with the synthesized peptide.
Western Blot: Rad51L1 Antibody (1 H3/13) [TA336269] - Western blot analysis of Rad51L1 expression in 1) HeLa, 2) HepG2 and 3) Cos 7 whole cell lysates.
WB Suggested Anti-RAD51L1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: COLO205 cell lysateRAD51B is supported by BioGPS gene expression data to be expressed in COLO205
WB Suggested Anti-RAD51L1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:1562500; Positive Control: HepG2 cell lysate
Host:Rabbit; Target Name:RAD51B; Sample Tissue:Human Fetal Brain; Antibody Dilution: 1.0 ug/ml
Host:Rabbit; Target Name:RAD51B; Sample Tissue:Human Adult Placenta; Antibody Dilution: 1.0 ug/ml
- Quick-Filters:
- Highly Validated (1)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA503550 | RAD51L1 antibody |
||||||
| RAD51L1 mouse monoclonal antibody, clone OTI7H4 (formerly 7H4) | |||||||
 |
Mouse | IgG1 | OTI7H4 | Purified | Hu | F, ICC/IF, WB |
0.1 ml /
€379.00
|
| OriGene Technologies, Inc. | |||||||
 +3 additional images |
Highly validated antibody!
|
||||||
| TA312603 | RAD51L1 antibody |
||||||
| Rabbit polyclonal anti-RAD51L1 antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms | E, P, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|||||||
| TA336269 | RAD51L1 antibody |
||||||
| Mouse Monoclonal Rad51L1 Antibody (1 H3/13) | |||||||
![Western Blot: Rad51L1 Antibody (1 H3/13) [TA336269] - Western blot analysis of Rad51L1 expression in 1) HeLa, 2) HepG2 and 3) Cos 7 whole cell lysates.](https://m1.acris-antibodies.com/catalog/product/cache/1/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/OriGene/NB100-176-1-W.jpg) |
Mouse | IgG2a | 1 H3/13 | Purified | Hu, Ms, Prim | WB |
0.1 ml /
€395.00
|
| OriGene Technologies, Inc. | |||||||
| AP20559PU-N | RAD51L1 antibody |
||||||
 |
Rabbit | Aff - Purified | Hu, Ms | P, WB |
0.1 mg /
€370.00
|
||
| OriGene Technologies GmbH | |||||||
| +3 additional images |
|||||||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP760598 | RAD51L1 (N-term HIS tag, transcript variant 3) |
||||
| Purified recombinant protein of Human RAD51-like 1 (S. cerevisiae) (RAD51L1), transcript variant 3, with N-terminal HIS tag, expressed in E.Coli, 50ug | |||||
 |
Human | > 80 % Preparation: . Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
E. coli |
50 µg /
€205.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY408768 | RAD51B overexpression lysate |
|
 |
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. |
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR220772L2V | Lenti ORF particles, Rad51b (GFP-tagged) - Mouse RAD51-like 1 (S. cerevisiae) (Rad51l1), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€930.00
|
|
| OriGene Technologies, Inc. | ||
| MR220772L1V | Lenti ORF particles, Rad51b (Myc-DDK-tagged) - Mouse RAD51-like 1 (S. cerevisiae) (Rad51l1), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€830.00
|
|
| OriGene Technologies, Inc. | ||
| MR203142L2V | Lenti ORF particles, Rad51l1 (GFP-tagged) - Mouse RAD51-like 1 (S. cerevisiae) (cDNA clone MGC:67754 IMAGE:5322190), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,240.00
|
|
| OriGene Technologies, Inc. | ||
| MR203142L1V | Lenti ORF particles, Rad51l1 (Myc-DDK-tagged) - Mouse RAD51-like 1 (S. cerevisiae) (cDNA clone MGC:67754 IMAGE:5322190), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,110.00
|
|
| OriGene Technologies, Inc. | ||