RALGDS antibody
Principal name
RALGDS antibody
Alternative names for RALGDS antibody
KIAA1308, RGF, RalGEF, Ral guanine nucleotide dissociation stimulator, Ral guanine nucleotide exchange factor
Gene ID
5900 (RALGDS)
Ncbi ID
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Immunoprecipitation (IP)
Background of RALGDS antibody
- Kit Component:
- - KN216933G1, RALGDS gRNA vector 1 in pCas-Guide vector
- KN216933G2, RALGDS gRNA vector 2 in pCas-Guide vector
- KN216933D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
Proximity Ligation Analysis (PLA) of protein-protein interactions between MAPK3 and RALGDS HeLa cells were stained with anti-MAPK3 rabbit purified polyclonal 1:1200 and anti-RALGDS mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.
Detection limit for recombinant GST tagged RALGDS is approximately 0.1ng/ml as a capture antibody.
Western Blot analysis of RALGDS expression in transfected 293T cell line (H00005900-T01) by RALGDS MaxPab polyclonal antibody.Lane 1: RALGDS transfected lysate(99.22 KDa).Lane 2: Non-transfected lysate.
Proximity Ligation Analysis (PLA) of protein-protein interactions between RALGDS and HRAS HeLa cells were stained with anti-RALGDS rabbit purified polyclonal 1:1200 and anti-HRAS mouse purified polyclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.
Western Blot analysis of RALGDS expression in transfected 293T cell line (H00005900-T01) by RALGDS MaxPab polyclonal antibody.Lane 1: RALGDS transfected lysate(99.30 KDa).Lane 2: Non-transfected lysate.
Human HepG2; WB Suggested Anti-RALGDS Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: HepG2 cell lysate; RALGDS antibody - N-terminal region (ARP56275_P050) in Human HepG2 cells using Western Blot
WB Suggested Anti-RALGDS Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: HepG2 cell lysate
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP316933 | RALGDS (transcript variant 1) |
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| Recombinant protein of human ral guanine nucleotide dissociation stimulator (RALGDS), transcript variant 1 | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€788.00
|
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| OriGene Technologies, Inc. | |||||