RANBP1 antibody
Principal name
RANBP1 antibody
Alternative names for RANBP1 antibody
Ran-binding protein 1, Ran-specific GTPase-activating protein
Ncbi ID
9606, NP_002873, NP_002873.1, NM_002882, NP_035369.2, NM_011239
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF)
Background of RANBP1 antibody
- Kit Component:
- - KN314454G1, Ranbp1 gRNA vector 1 in pCas-Guide vector
- KN314454G2, Ranbp1 gRNA vector 2 in pCas-Guide vector
- KN314454D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Hayashi N., et al. (1995) Mol Gen Genet. 247(6):661-9.Ren M., et al. (1995) Mol Cell biol. 15(4):2177-24.
RANBP1 polyclonal antibody ( Cat # PAB6654 ) staining ( 0.1 µg/ml ) of HepG2 lysate ( RIPA buffer, 35 µg total protein per lane ) . Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Human 721_B; WB Suggested Anti-RANBP1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:12500. Positive Control: 721_B cell lysate. RANBP1 is strongly supported by BioGPS gene expression data to be expressed in Human 721_B cells.; RANBP1 antibody - C-terminal region (ARP46083_P050) in Human 721_B cells using Western Blot
Western blot analysis of RANBP1 expression in HeLa (A); mouse brain (B); rat brain (C) whole cell lysates.
TA302686 (0.05µg/ml) staining of HELA lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.chemiluminescence.
TA302686 (3.8µg/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Sample (50 ug of whole cell lysate). A: Mouse brain. 12% SDS PAGE. TA308769 diluted at 1:1000.
Sample (30 ug of whole cell lysate). A: HeLa S3 . 12% SDS PAGE. TA308769 diluted at 1:1000
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Ran BP-1 (TA308769) antibody at 1:200 dilution.
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA302686 | RANBP1 (C-term) antibody |
||||||
| Goat Polyclonal Antibody against RANBP1 | |||||||
 |
Goat | Aff - Purified | Hu | E, P, WB |
0.1 mg /
€360.00
|
||
| OriGene Technologies, Inc. | |||||||
 +1 additional image |
|||||||
| TA308769 | RANBP1 (1-187) antibody |
||||||
| Rabbit Polyclonal antibody to Ran BP1 (RAN binding protein 1) | |||||||
 |
Rabbit | Aff - Purified | Hu, Ms | ICC/IF, WB |
0.1 ml /
€415.00
|
||
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
|||||||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP323305 | RANBP1 |
||||
| Recombinant protein of human RAN binding protein 1 (RANBP1) | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
| TP710020 | RANBP1 |
||||
| Recombinant protein of human v-raf-1 murine leukemia viral oncogene homolog 1 (RAF1),residues 30polyhistidine tag,expressed in sf9 cells. | |||||
 |
Human | > 80 % Preparation: . Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
SF9 cells |
20 µg /
€411.00
|
|
| OriGene Technologies, Inc. | |||||
| TP760657 | RANBP1 (full length, N-term HIS tag) |
||||
| Purified recombinant protein of Human RAN binding protein 1 (RANBP1), full length, with N-terminal HIS tag, expressed in E.Coli, 50ug | |||||
 |
Human | > 80 % Preparation: . Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
E. coli |
50 µg /
€205.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY401015 | RANBP1 overexpression lysate |
|
 |
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. |
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR202097L2V | Lenti ORF particles, Ranbp1 (GFP-tagged) - Mouse RAN binding protein 1 (Ranbp1), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€930.00
|
|
| OriGene Technologies, Inc. | ||
| MR202097L1V | Lenti ORF particles, Ranbp1 (Myc-DDK-tagged) - Mouse RAN binding protein 1 (Ranbp1), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€830.00
|
|
| OriGene Technologies, Inc. | ||