RanGAP1 antibody
Principal name
RanGAP1 antibody
Alternative names for RanGAP1 antibody
Ran GTPase-activating protein 1, KIAA1835, segregation distorter homolog, SD
Gene ID
5905 (RANGAP1)
Ncbi ID
Available reactivities
Available hosts
Available applications
ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E), Flow Cytometry (F)
Background of RanGAP1 antibody
- Kit Component:
- - KN204223G1, RANGAP1 gRNA vector 1 in pCas-Guide vector
- KN204223G2, RANGAP1 gRNA vector 2 in pCas-Guide vector
- KN204223D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Bischoff FR, Krebber H, Kempf T, Hermes I, Ponstingl H.
Human RanGTPase-activating protein RanGAP1 is a homologue of yeast Rna1p involved in mRNA processing and transport.
Proc Natl Acad Sci U S A. 1995 Feb 28;92(5):1749-53.
Immunoperoxidase of purified MaxPab antibody to RANGAP1 on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3 ug/ml]
Immunofluorescence of purified MaxPab antibody to RANGAP1 on HeLa cell. [antibody concentration 10 ug/ml]
Western Blot analysis of RANGAP1 expression in transfected 293T cell line (H00005905-T01) by RANGAP1 MaxPab polyclonal antibody.Lane 1: RANGAP1 transfected lysate(64.57 KDa).Lane 2: Non-transfected lysate.
RANGAP1 MaxPab polyclonal antibody. Western Blot analysis of RANGAP1 expression in Jurkat.
RANGAP1 polyclonal antibody ( Cat # PAB6571 ) staining ( 0.2 µg/ml ) of NIH/3T3 lysate ( RIPA buffer, 35 µg total protein per lane ) . Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Immunoperoxidase of purified MaxPab antibody to RANGAP1 on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3 ug/ml]
Immunofluorescence of purified MaxPab antibody to RANGAP1 on HeLa cell. [antibody concentration 10 ug/ml]
Western Blot analysis of RANGAP1 expression in transfected 293T cell line (H00005905-T01) by RANGAP1 MaxPab polyclonal antibody.Lane 1: RANGAP1 transfected lysate(64.57 KDa).Lane 2: Non-transfected lysate.
RANGAP1 MaxPab polyclonal antibody. Western Blot analysis of RANGAP1 expression in Jurkat.
Western blot of RCC1 polyclonal antibody ( Cat # PAB12339 ), RanGAP polyclonal antibody ( Cat # PAB12338 ) and RAN polyclonal antibody ( Cat # PAB12357 ) on Hela total cell and Xenopus egg extract.
Human Muscle; WB Suggested Anti-RANGAP1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Human Muscle; RANGAP1 antibody - N-terminal region (ARP56509_P050) in Human Muscle cells using Western Blot
Human Testis (formalin-fixed, paraffin-embedded) stained with RANGAP1 antibody AP22630SU-N at 1/100 followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
Human Skin (formalin-fixed, paraffin-embedded) stained with RANGAP1 antibody AP22630SU-N at 1/100 followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-RANGAP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501265, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-RANGAP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501265, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-RANGAP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501265, Dilution 1:50)
Anti-RANGAP1 mouse monoclonal antibody (TA501265) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY RANGAP1(RC204223).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RANGAP1 (RC204223, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RANGAP1.
Western blot analysis of extracts (10ug) from 10 Human tissue by using anti-RANGAP1 monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon;10: spleen).
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-RANGAP1 monoclonal antibody.
Flow cytometric Analysis of Jurkat cells, using anti-RANGAP1 antibody(TA501265),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
Flow cytometric Analysis of Hela cells, using anti-RANGAP1 antibody(TA501265),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
HEK293T cells transfected with either RC204223 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-RANGAP1 antibody(TA501265), and then analyzed by flow cytometry.
TA302687 staining (0.2µg/ml) of 3T3 lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Western blot analysis of extracts of various cell lines, using RANGAP1 antibody.
Immunohistochemistry of paraffin-embedded human liver cancer using RANGAP1 antibody at dilution of 1:200 (400x lens).
Immunohistochemistry of paraffin-embedded human lung using RANGAP1 antibody at dilution of 1:200 (400x lens).
Immunofluorescence analysis of U20S cell using RANGAP1 antibody. Blue: DAPI for nuclear staining.
WB Suggested Anti-RANGAP1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: Human Muscle
- Quick-Filters:
- Highly Validated (2)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA501265 | RanGAP1 antibody |
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| anti-RANGAP1 mouse monoclonal antibody, clone OTI1B4 (formerly 1B4) | |||||||
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Mouse | IgG2a | OTI1B4 | Purified | Can, Hu, Rt | F, ICC/IF, P, WB |
0.1 ml /
€379.00
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| OriGene Technologies, Inc. | |||||||
 +9 additional images |
Top Selling product! Highly validated antibody!
|
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| TA332656 | RanGAP1 antibody |
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| Rabbit anti-RANGAP1 Polyclonal Antibody | |||||||
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Rabbit | IgG | Purified | Hu, Ms, Rt |
0.1 mg /
€335.00
|
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| OriGene Technologies, Inc. | |||||||
| +3 additional images |
Highly validated antibody! |
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| TA302687 | RanGAP1 (N-term) antibody |
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| Goat Polyclonal Antibody against RANGAP1 | |||||||
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Goat | Aff - Purified | Bov, Can, Hu, Ms, Rt | E, P, WB |
0.1 mg /
€360.00
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| OriGene Technologies, Inc. | |||||||
| AP22630SU-N | RanGAP1 antibody |
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Rabbit | Serum | Hu, Xen | ICC/IF, P, WB |
50 µl /
€425.00
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| OriGene Technologies GmbH | |||||||
| +1 additional image |
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Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP304223 | RanGAP1 |
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| Recombinant protein of human Ran GTPase activating protein 1 (RANGAP1) | |||||
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Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
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| OriGene Technologies, Inc. | |||||