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RAP1GAP antibody

Principal name

RAP1GAP antibody

Alternative names for RAP1GAP antibody

Rap1GAP1, RAP1GA1, KIAA0474

SwissProt ID

P47736 (Human)

Gene ID

5909 (RAP1GAP)

Ncbi ID

9606, NP_002876, NP_001139129.1, NM_001145657, NM_001145658

Available reactivities

Hu (Human), Bov (Bovine), Ms (Mouse), Rt (Rat), Ze (Zebrafish), African clawed frog, Eq (Equine), GP (Guinea Pig), Rb (Rabbit)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Flow Cytometry (F), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of RAP1GAP antibody

This gene encodes a type of GTPase-activating-protein (GAP) that down-regulatesThe activity ofThe ras-related RAP1 protein. RAP1 acts as a molecular switch by cycling between an ictive GDP-bound form and an active GTP-bound form.The product ofThis gene, RAP1GAP, promotesThe hydrolysis of bound GTP and hence returns RAP1 toThe ictive state whereas other proteins, guanine nucleotide exchange factors (GEFs), act as RAP1 activators by facilitatingThe conversion of RAP1 fromThe GDP- toThe GTP-bound form. In general, ras subfamily proteins, such as RAP1, play key roles in receptor-linked sigling pathways that control cell growth and differentiation. RAP1 plays a role in diverse processes such as cell proliferation, adhesion, differentiation, and embryogenesis. Altertive splicing results in multiple transcript variants encoding distinct proteins. [provided by RefSeq, Aug 2011].

Detection limit for recombinant GST tagged RAP1GA1 is approximately 1ng/ml as a capture antibody.

H00005909-M02

Human 721_B; Host:Rabbit. Target Name:RAP1GAP. Sample Tissue:Human 721_B. Antibody Dilution: 1.0ug/ml. RAP1GAP is supported by BioGPS gene expression data to be expressed in 721_B.; RAP1GAP antibody - middle region (ARP56511_P050) in Human 721_B cells using Western Blot

ARP56511_P050

Human Hela; Host:Rabbit. Target Name:RAP1GAP. Sample Tissue:Human Hela. Antibody Dilution: 1.0ug/ml. RAP1GAP is supported by BioGPS gene expression data to be expressed in HeLa.; RAP1GAP antibody - middle region (ARP56511_P050) in Human Hela cells using Western Blot

ARP56511_P050

Human 293T; WB Suggested Anti-RAP1GAP Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: 293T cell lysate. RAP1GAP is supported by BioGPS gene expression data to be expressed in HEK293T.; RAP1GAP antibody - middle region (ARP56511_P050) in Human 293T cells using Western Blot

ARP56511_P050

Transfected 293T; WB Suggested Anti-RAP1GAP Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: Transfected 293T; RAP1GAP antibody - middle region (ARP56512_P050) in Transfected 293T cells using Western Blot

ARP56512_P050

Immunohistochemical analysis of RAP1GAP staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Western blot analysis of RAP1GAP expression in Jurkat (A); HeLa (B); mouse brain (C) whole cell lysates.

TP308271

Western blot analysis of extracts from COLO205 cells, using RAP1GDS1 antibody.The lane on the right is treated with the synthesized peptide.

TA313043

WB Suggested Anti-RAP1GAP Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: 293T cell lysateRAP1GAP is supported by BioGPS gene expression data to be expressed in HEK293T

TA342187

Host:Rabbit; Target Name:RAP1GAP; Sample Tissue:Human 721_B; Antibody Dilution: 1.0 ug/mlRAP1GAP is supported by BioGPS gene expression data to be expressed in 721_B

TA342187

Host:Rabbit; Target Name:RAP1GAP; Sample Tissue:Human Hela; Antibody Dilution: 1.0 ug/mlRAP1GAP is supported by BioGPS gene expression data to be expressed in HeLa

TA342187

WB Suggested Anti-RAP1GAP Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:1562500; Positive Control: Transfected 293T

TA342188

Immunofluorescence staining of MEFs with anti-RAP1 [RAP1 4C8/1] antibody.

TA353430

Primary Antibodies

Catalog No.	Host	Iso.	Pres.	React.	Applications
TA313043	RAP1GAP antibody
	Rabbit polyclonal anti-RAP1GDS1 antibody
	Rabbit	IgG	Purified	Hu, Ms	E, WB	0.1 ml / €335.00
						OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No.	Species	Purity	Source
TP308271	RAP1GAP (transcript variant 3)
	Recombinant protein of human RAP1 GTPase activating protein (RAP1GAP), transcript variant 3
	Human	> 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.	HEK293 cells	20 µg / €748.00
				OriGene Technologies, Inc.

Lysates

Catalog No.
LY428957	RAP1GAP overexpression lysate
		0.1 mg / €495.00
		OriGene Technologies, Inc.

Human Lenti ORF Particles

Catalog No.
RC226868L2V	Lenti ORF particles, RAP1GAP (mGFP-tagged)-Human RAP1 GTPase activating protein (RAP1GAP), transcript variant 1, 200 uL, >10^7 TU/mL
		200 µl / €1,710.00
		OriGene Technologies, Inc.

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