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RAPTOR antibody

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Principal name

RAPTOR antibody

Alternative names for RAPTOR antibody

RPTOR, KIAA1303, Regulatory-associated protein of mTOR, P150 target of rapamycin TOR-scaffold protein

SwissProt ID

Q55BR7 (Dicdi), Q8K4Q0 (Mouse), Q8N122 (Human)

Gene ID

57521 (RPTOR)

Ncbi ID

NP_065812.1, 9606, 10090, NP_065812, Q8K4Q0, Q8N122, NM_020761, NM_001163034

Available reactivities

Hu (Human), Ms (Mouse), Can (Canine), Rt (Rat)

Available hosts

Rabbit, Mouse

Available applications

Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), Dot blot (Dot), Enzyme Immunoassay (E), ELISA (detection) (E(detection)), Immunoprecipitation (IP)

Background of RAPTOR antibody

The mammalian Target of Rapamycin (TOR, also known as mTOR) is an evolutionarily conserved serine/threonine kinase that regulates cell growth and cell cycle through its ability to integrate signals from nutrient levels and growth factors (reviewed in 1). Rapamycin inhibits TOR activity resulting in reduced cell growth and reduced rates of cell cycle and cell proliferation (reviewed in 2). Raptor (regulatory associated protein of TOR) is a TOR-binding protein essential for TOR signaling in vivo. It acts as a TOR scaffold protein whose binding by TOR substrates is necessary for effective TOR-catalyzed phosphorylation (3). These substrates include the ribosomal protein S6 kinase (RP S6K) and the eukaryotic initiation factor 4E binding protein 4EBP1, proteins necessary for cell growth and proliferation and responsive to nutrient and mitogen levels (4). Raptor binds these proteins through a common 5 amino acid TOR-signaling (TOS) motif; mutation of this motif prevents the TOR-dependent phosphorylation of these proteins (5).

General readings

Cell lysate from untreated HeLa cells, epitheloid cervical carcinoma (human)

AP07462PU-N - RAPTOR
Kidney: Formalin-Fixed Paraffin-Embedded (FFPE)
AP07764PU-N - RAPTOR
AP07764PU-N Raptor antibody staining of Formalin-Fixed Paraffin-Embedded Human Brain, Cortex.
AP07764PU-N - RAPTOR
AP07764PU-N Raptor antibody staining of Formalin-Fixed Paraffin-Embedded Human Spleen.
AP07764PU-N - RAPTOR
Western blot analysis of Raptor in L1210 cell lysate with AP07764PU-N Raptor antibody 2 µg/ml (A) and 4 µg/ml (B).
PAB1647 - RAPTOR
Dot blot analysis of KIAA1303 (phospho S863) polyclonal antibody ( Cat # PAB1647 ) on nitrocellulose membrane. 50 ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5 µg/ml.
AP12910PU-N - RAPTOR
Dot blot analysis of anti-raptor-pSer863 Pab (Cat#AP12910PU-N) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.
H00057521-B01P - RAPTOR
Immunofluorescence of purified MaxPab antibody to RPTOR on HeLa cell. [antibody concentration 10 ug/ml]
H00057521-B01P - RAPTOR
Western Blot analysis of KIAA1303 expression in transfected 293T cell line (H00057521-T04) by KIAA1303 MaxPab polyclonal antibody.Lane 1: RPTOR transfected lysate(41.69 KDa).Lane 2: Non-transfected lysate.
PAB13417 - RAPTOR
Immunocytochemistry of Rptor in L1210 cells with Rptor polyclonal antibody ( Cat # PAB13417 ) at 10 µg/ml.
PAB13417 - RAPTOR
Western blot analysis of Rptor in L1210 cell lysate with Rptor polyclonal antibody ( Cat # PAB13417 ) at ( A ) 2 and ( B ) 4 µg/ml.
PAB13418 - RAPTOR
Immunocytochemistry of RPTOR in L1210 cells with RPTOR polyclonal antibody ( Cat # PAB13418 ) at 10 µg/ml.
PAB13418 - RAPTOR
Western blot analysis of RPTOR in L1210 cell lysate with RPTOR polyclonal antibody ( Cat # PAB13418 ) at ( A ) 2 and ( B ) 4 µg/ml.
PAB13419 - RAPTOR
Immunocytochemistry staining of L1210 cells using RPTOR polyclonal antibody ( Cat # PAB13419 ) at 1µg/ml
PAB13419 - RAPTOR
Western blot analysis of RPTOR in L1210 cell lysate with RPTOR polyclonal antibody ( Cat # PAB13419 ) at ( A ) 2 and ( B ) 4 µg/ml.
H00057521-D01 - RAPTOR
Immunoprecipitation of RPTOR transfected lysate using anti-RPTOR MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with RPTOR MaxPab mouse polyclonal antibody (B01) (H00057521-B01).
AM20221PU-N - RAPTOR
Detection of endogenous Raptor: Whole cell lysates of serum starved tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to PVDF membranes. Immunoblots were probed with AM20221PU-N Raptor antibody (Clone 10E10) at 0.5 µg/ml for 1h at RT and developed by ECL (exp. time: 30 sec).Lane 1: HeLa Lane 2: HepG2 Lane 3: HEK293Lane 4: SH-SY5Y Lane 5: MDCK Lane 6: PC12Lane 7: CMT-93 Lane 8: Neuro 2A Lane 9: 3T3
AP22943PU-N - RAPTOR
Human Skeletal Muscle: Formalin-Fixed, Paraffin-Embedded (FFPE)
AP22943PU-N - RAPTOR
Immunocytochemistry in L1210 cells with Raptor antibody (AP22943PU-N) at 10 µg/ml.
AP22943PU-N - RAPTOR
Western blot analysis of Raptor in L1210 cell lysate with Raptor antibody (AP22943PU-N) at (A) 2 and (B) 4 µg/ml
TA306211 - RAPTOR
Western blot analysis of Raptor in L1210 cell lysate with Raptor antibody at (A) 2 and (B) 4 ug/ml.
TA306211 - RAPTOR
Immunohistochemistry of Raptor in L1210 cells with Raptor antibody at 10 ug/ml.
TA319977 - RAPTOR
Western blot analysis of Raptor in L1210 cell lysate with Raptor antibody at (A) 2 and (B) 4 ug/mL.
TA319977 - RAPTOR
Immunocytochemistry of RAPTOR in L1210 cells with RAPTOR antibody at 10 ug/mL.
TA319977 - RAPTOR
Immunofluorescence of Raptor in L1210 cells with Raptor antibody at 10 ug/mL.
TA319978 - RAPTOR
Western blot analysis of Raptor in L1210 cell lysate with Raptor (IN) antibody at (A) 2 and (B) 4 ug/mL.
TA319978 - RAPTOR
Immunocytochemistry staining of L1210 cells using RAPTOR antibody at 1ug/mL
TA319978 - RAPTOR
Immunofluorescence of Raptor in L1210 cells with Raptor antibody at 10 ug/mL.
TA328101 - RAPTOR
293T cell extract was resolved by electrophoresis, transferred to nitrocellulose, and probed with rabbit anti-Raptor antibody. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system.
TA328101 - RAPTOR
Formalin-fixed paraffin-embedded human kidney tissue was stained with Poly6232 and developed with an alkaline phosphatase chromogen substate (red color). Tissue was counterstained with H&E (blue/pink). Magnification, 40X.
TA337071 - RAPTOR
Western Blot: Raptor Antibody [TA337071] - Western blot analysis of Raptor in A) human 293 and B) mouse NIH 3T3 lysate using Raptor antibody at 1:2000.
TA337071 - RAPTOR
Immunohistochemistry-Paraffin: Raptor Antibody [TA337071] - IHC analysis of human small intestine using Raptor antibody at 1:100.

10 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

RAPTOR (C-term) antibody
  Rabbit Polyclonal Raptor Antibody  
Western blot analysis of Raptor in L1210 cell lysate with Raptor antibody at (A) 2 and (B) 4 ug/ml. Rabbit IgG Aff - Purified Ms E, ICC/IF, WB
0.1 mg / €415.00
  OriGene Technologies, Inc.
+1 additional image

RAPTOR (N-term) antibody
  Rabbit Polyclonal Raptor Antibody  
Western blot analysis of Raptor in L1210 cell lysate with Raptor antibody at (A) 2 and (B) 4 ug/mL. Rabbit IgG Aff - Purified Hu, Ms E, ICC/IF, WB
0.1 mg / €415.00
  OriGene Technologies, Inc.
+2 additional images

RAPTOR antibody
  Rabbit Polyclonal anti-Raptor Antibody  
293T cell extract was resolved by electrophoresis, transferred to nitrocellulose, and probed with rabbit anti-Raptor antibody. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system. Rabbit IgG Poly6232 Purified Hu P, WB
0.2 ml / €275.00
  OriGene Technologies, Inc.
+1 additional image

RAPTOR antibody
  Rabbit Polyclonal RPTOR Antibody  
Western Blot: Raptor Antibody [TA337071] - Western blot analysis of Raptor in A) human 293 and B) mouse NIH 3T3 lysate using Raptor antibody at 1:2000. Rabbit Purified Hu, Ms P, WB
0.2 ml / €395.00
  OriGene Technologies, Inc.
+1 additional image

RAPTOR antibody
Kidney: Formalin-Fixed Paraffin-Embedded (FFPE) Rabbit IgG Aff - Purified Hu P, WB
50 µl / €425.00
  OriGene Technologies GmbH

RAPTOR antibody
AP07764PU-N Raptor antibody staining of Formalin-Fixed Paraffin-Embedded Human Brain, Cortex. Rabbit Aff - Purified Hu, Ms ICC/IF, P, WB
50 µg / €425.00
  OriGene Technologies GmbH
+2 additional images

RAPTOR pSer863 antibody
Dot blot analysis of anti-raptor-pSer863 Pab (Cat#AP12910PU-N) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml. Rabbit Ig Aff - Purified Hu
0.4 ml / €415.00
  OriGene Technologies GmbH

RAPTOR (N-term) antibody
Human Skeletal Muscle: Formalin-Fixed, Paraffin-Embedded (FFPE) Rabbit Purified Hu, Ms ICC/IF, P, WB
50 µg / €425.00
  OriGene Technologies GmbH
+2 additional images

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, RPTOR (mGFP-tagged)-Human regulatory associated protein of MTOR, complex 1 (RPTOR), transcript variant 2, 200 uL, >10^7 TU/mL
Lenti ORF particles, RPTOR (mGFP-tagged)-Human regulatory associated protein of MTOR, complex 1 (RPTOR), transcript variant 2, 200 uL, >10^7 TU/mL  
200 µl / €2,470.00
  OriGene Technologies, Inc.
 

Lenti ORF particles, RPTOR (Myc-DDK-tagged)-Human regulatory associated protein of MTOR, complex 1 (RPTOR), transcript variant 2, 200 uL, >10^7 TU/mL
Lenti ORF particles, RPTOR (Myc-DDK-tagged)-Human regulatory associated protein of MTOR, complex 1 (RPTOR), transcript variant 2, 200 uL, >10^7 TU/mL  
200 µl / €2,230.00
  OriGene Technologies, Inc.
 

10 Item(s)

per page

Available Filters

Product Type
  1. Human Lenti ORF Particle (2)
  2. Primary Antibody (8)
Clonality
  1. Polyclonal (8)
Target
  1. RAPTOR (8)
Property
  1. C-term (1)
  2. N-term (2)
  3. pSer863 (1)
Reactivity
  1. Human (Hu) (7)
  2. Mouse (Ms) (5)
Applications
  1. Western blot / Immunoblot (WB) (7)
  2. Paraffin Sections (P) (5)
  3. Immunocytochemistry/​Immunofluorescence (ICC/IF) (4)
  4. Enzyme Immunoassay (E) (2)
Presentation
  1. Aff - Purified (5)
  2. Purified (3)
Host
  1. Rabbit (8)
Images
  1. 1 image (8)
  2. 3+ images (3)
Images: Applications
  1. Western blot / Immunoblot (WB) (6)
  2. IHC (3)
  3. Paraffin Sections (P) (3)
  4. Dot blot (Dot) (1)
  5. Immunocytochemistry/​Immunofluorescence (ICC/IF) (1)
  6. Immunofluorescence (IF) (1)
Manufacturer
  1. OriGene Technologies (10)
Service
  • OriGene Technologies GmbH
  • Schillerstraße 5
  • D-32052 Herford
  • Germany
  • phone: +49-5221-34606-0
  • free call: 0800-227 47 46 (Germany only)
  • fax: +49-5221-34606-11
  • email: info-de@origene.com
  • orders: ordering-de@origene.com
 
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