RASGRP2 antibody
Principal name
RASGRP2 antibody
Alternative names for RASGRP2 antibody
CDC25L, MCG7, CalDAG-GEFI, RAS guanyl-releasing protein 2, Cdc25-like protein, Calcium and DAG-regulated guanine nucleotide exchange factor I
Ncbi ID
9606, NP_722541, NP_001092140.1, NM_153819, NM_001098670, NM_001098671, NP_001092140, NP_001092141, NP_035372.2, NM_011242
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF)
Background of RASGRP2 antibody
- Kit Component:
- - KN314504G1, Rasgrp2 gRNA vector 1 in pCas-Guide vector
- KN314504G2, Rasgrp2 gRNA vector 2 in pCas-Guide vector
- KN314504D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
Detection limit for recombinant GST tagged RASGRP2 is approximately 0.3ng/ml as a capture antibody.
RASGRP2 polyclonal antibody (A01), Lot # NIH47060208QCS1 Western Blot analysis of RASGRP2 expression in A-431 ( Cat # L015V1 ).
Human Brain; WB Suggested Anti-RASGRP2 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Human brain; RASGRP2 antibody - N-terminal region (ARP58820_P050) in Human Brain cells using Western Blot
RASGRP2 Antibody (N-term) (Cat. #TA324479) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the RASGRP2 antibody detected the RASGRP2 protein (arrow).
RASGRP2 Antibody (N-term) (Cat. #TA324479)immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of RASGRP2 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Confocal immunofluorescent analysis of RASGRP2 Antibody (N-term)(Cat#TA324479) with U-251MG cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).
WB Suggested Anti-RASGRP2 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: Human brain
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Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA324479 | RASGRP2 (N-term) antibody |
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| Rabbit polyclonal RASGRP2 Antibody (N-term) | |||||||
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Rabbit | Ig | Aff - Purified | Hu | ICC/IF, P, WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies, Inc. | |||||||
 +2 additional images |
Published recently! |
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Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP312672 | RASGRP2 (transcript variant 3) |
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| Recombinant protein of human RAS guanyl releasing protein 2 (calcium and DAG-regulated) (RASGRP2), transcript variant 3 | |||||
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Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
| TP312719 | RASGRP2 (transcript variant 4) |
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| Recombinant protein of human RAS guanyl releasing protein 2 (calcium and DAG-regulated) (RASGRP2), transcript variant 4 | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
| TP317525 | RASGRP2 (transcript variant 2) |
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| Recombinant protein of human RAS guanyl releasing protein 2 (calcium and DAG-regulated) (RASGRP2), transcript variant 2 | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY406966 | RASGRP2 overexpression lysate |
|
 |
0.1 mg /
€495.00
|
|
| OriGene Technologies, Inc. | ||
| LY420662 | RASGRP2 overexpression lysate |
|
|
0.1 mg /
€495.00
|
|
| OriGene Technologies, Inc. | ||
| LY420663 | RASGRP2 overexpression lysate |
|
|
0.1 mg /
€495.00
|
|
| OriGene Technologies, Inc. | ||
| LY426039 | RASGRP2 overexpression lysate |
|
|
0.1 mg /
€315.00
|
|
| OriGene Technologies, Inc. | ||
| LY426040 | RASGRP2 overexpression lysate |
|
|
0.1 mg /
€315.00
|
|
| OriGene Technologies, Inc. |