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Home » Target » Rat IgM antibody

Rat IgM antibody

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Principal name

Rat IgM antibody

Alternative names for Rat IgM antibody

Rat Immunoglobulin M

Available reactivities

Rt (Rat)

Available hosts

Rabbit, Goat, Mouse

Available applications

Enzyme Immunoassay (E), Flow Cytometry (F), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Functional assay (FN), Paraffin Sections (P), Frozen Sections (C)

Background of Rat IgM antibody

IgM normally constitutes about 10% of serum immunoglobulins. IgM antibody is prominent in early immune responses to most antigens and predominates in certain antibody responses such as 'natural' blood group antibodies. IgM (with IgD) is the major immunoglobulin expressed on the surface of B cells. The gene for the mu constant region contains four domains separated by short intervening sequences.
Class specific anti immunoglobulin antibodies are useful for: The characterization of malignant B cell proliferations. All but acute lymphocytic leukemias share either surface or intra cytoplasmic Ig with an isotypic restriction, which suggest the monoclonal nature of the cell population. Most of the chronic lymphocytic leukemias, non Hodgkin lymphomas and Burkitt's lymphoma bear surface IgM, whereas plasmocytes from Waldenström's disease bear intracytoplasmic IgM. The other isotypes are less frequently found. On the other hand multiple myelomas are usually of the IgG or IgA type.
Characterization of plasma cells in inflammatory conditions: Plasma cell typing can be of use for the classification of intestinal inflammatory conditions such as inflammatory bowel disease and allergic conditions. In the latter a specific increase in the number of IgE plasma cells can be demonstrated.

AM08095RP-N - Rat IgM
Immunofluorescent Staining: BALB/c splenocytes were incubated with Rat IgM Isotype Control, Rat anti-Mouse CD22 (2D6, Rat IgG1), Rat anti-Mouse CD8 (53-6.7, Rat IgG2a), Rat anti-Mouse CD45 (LCA) (I3/2.3, Rat IgG2b), Rat anti-Mouse CD3(C363.29B, Rat IgG2c), and Rat anti-Mouse CD45RC (GL24, rat IgM). After washing, the cells were stained with PE-labeled Mouse Anti-Rat IgM. Small Lymphocytes were gated and analyzed on a FACScan(TM) flow cytometer (BDIS, San Jose, CA). Amount Used: < / = 0.02 µg/10e6 cells.
AM08096PU-N - Rat IgM
B Cell Stimulation: 5 x 10e5 SD Rat splenocytes were incubated with the indicated concentrations of purified Mouse F(ab')2 Anti-Rat IgM, clone M2A1, for 48 hours. B cell proliferation was determined by 3H-thymidineincorporation assay. Amount Used: < / = 10 μg/mL

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Secondary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Rat IgM (Mu chain specific)
Rat IgM Mouse IgG1 Biotin Rt E, F, ICC/IF
0.5 mg / €330.00
  OriGene Technologies GmbH

Rat IgM (Mu chain specific)
Rat IgM Mouse IgG1 M 2A1 Cy5 Rt F, ICC/IF
0.1 mg / €450.00
  OriGene Technologies GmbH

Rat IgM (Mu chain specific)
Rat IgM Mouse IgG1 M 2A1 FITC Rt F, ICC/IF
0.5 mg / €360.00
  OriGene Technologies GmbH

Rat IgM (Mu chain specific)
Rat IgM Mouse IgG1 M 2A1 HRP Rt E
1 ml / €430.00
  OriGene Technologies GmbH

Rat IgM (Mu chain specific)
Rat IgM Mouse IgG1 M 2A1 Purified E, F, ICC/IF
0.5 mg / €300.00
  OriGene Technologies GmbH

Rat IgM (Mu chain specific)
Immunofluorescent Staining: BALB/c splenocytes were incubated with Rat IgM Isotype Control, Rat anti-Mouse CD22 (2D6, Rat IgG1), Rat anti-Mouse CD8 (53-6.7, Rat IgG2a), Rat anti-Mouse CD45 (LCA) (I3/2.3, Rat IgG2b), Rat anti-Mouse CD3(C363.29B, Rat IgG2c), and Rat anti-Mouse CD45RC (GL24, rat IgM). After washing, the cells were stained with PE-labeled Mouse Anti-Rat IgM. Small Lymphocytes were gated and analyzed on a FACScan(TM) flow cytometer (BDIS, San Jose, CA). Amount Used: < / = 0.02 µg/10e6 cells. Mouse IgG1 M 2A1 PE Rt F, ICC/IF
0.1 mg / €340.00
  OriGene Technologies GmbH

Rat IgM Mu chain specific F(ab’)2 Fragment
Rat IgM Mouse IgG1 M 2A1 Low Endotoxin Rt FN
0.5 mg / €450.00
  OriGene Technologies GmbH

Rat IgM Mu chain specific F(ab’)2 Fragment
B Cell Stimulation: 5 x 10e5 SD Rat splenocytes were incubated with the indicated concentrations of purified Mouse F(ab')2 Anti-Rat IgM, clone M2A1, for 48 hours. B cell proliferation was determined by 3H-thymidine incorporation assay. Amount Used: < / = 10 μg/mL Mouse IgG1 M 2A1 Purified Rt FN
0.5 mg / €400.00
  OriGene Technologies GmbH

Rat IgM (Mu chain specific)
Rat IgM Mouse IgG1 HIS40 Supernatant Rt C, ICC/IF
1 ml / €280.00
  OriGene Technologies GmbH

Rat IgM
Rat IgM Goat IgG HRP Rt E, WB
0.5 mg / €300.00
  OriGene Technologies GmbH

Rat IgM
Rat IgM Goat IgG Purified Rt E, F, WB
0.5 mg / €240.00
  OriGene Technologies GmbH

Rat IgM (Mu chain specific)
Rat IgM Rabbit AP
1 mg / €370.00
  OriGene Technologies GmbH

Rat IgM (Mu chain specific)
Rat IgM Rabbit Biotin
1.5 mg / €305.00
  OriGene Technologies GmbH

Rat IgM (Mu chain specific)
Rat IgM Rabbit FITC
1.5 mg / €245.00
  OriGene Technologies GmbH

Rat IgM (Mu chain specific)
Rat IgM Rabbit HRP
1.5 mg / €330.00
  OriGene Technologies GmbH

Rat IgM (Mu chain specific)
Rat IgM Rabbit Aff - Purified
2 mg / €245.00
  OriGene Technologies GmbH

Rat IgM (Mu chain specific)
Rat IgM Rabbit TRITC
1.5 mg / €250.00
  OriGene Technologies GmbH

Rat IgM (Mu chain specific)
Rat IgM Rabbit Texas Red
1.5 mg / €305.00
  OriGene Technologies GmbH

Rat IgM (Mu chain specific)
Rat IgM Goat PE
1 ml / €415.00
  OriGene Technologies GmbH

Rat IgM (heavy chain)
Rat IgM Mouse IgG1 MARM-4 Biotin Rt E
0.5 mg / €610.00
  OriGene Technologies GmbH

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25 products - FILTER YOUR RESULTS!

Available Filters

Product Type
  1. Protein | Growth Factor (2)
  2. Secondary Antibody (23)
Clonality
  1. Monoclonal (13)
  2. Polyclonal (10)
Target
  1. Rat IgM (25)
Reactivity
  1. Rat (Rt) (14)
Applications
  1. Enzyme Immunoassay (E) (8)
  2. Flow cytometry (F) (7)
  3. Immunocytochemistry/​Immunofluorescence (ICC/IF) (6)
  4. Functional assay (FN) (2)
  5. Western blot / Immunoblot (WB) (2)
  6. Frozen sections (C) (1)
Presentation
  1. HRP (4)
  2. Purified (4)
  3. Biotin (3)
  4. FITC (3)
  5. PE (2)
  6. AP (1)
  7. Aff - Purified (1)
  8. Cy5 (1)
  9. Low Endotoxin (1)
  10. Supernatant (1)
  11. TRITC (1)
  12. Texas Red (1)
Host
  1. Mouse (13)
  2. Rabbit (7)
  3. Goat (3)
Images
  1. 1 image (2)
Images: Applications
  1. Flow cytometry (F) (1)
  2. Functional assay (FN) (1)
Species (Protein)
  1. Rat (2)
Source
  1. Myeloma (1)
  2. Serum (1)
Manufacturer
  1. OriGene Technologies (25)
Service
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