RBMS1 antibody
Principal name
RBMS1 antibody
Alternative names for RBMS1 antibody
C2orf12, MSSP, MSSP1, SCR2, RNA-binding motif single-stranded-interacting protein 1, Single-stranded DNA-binding protein MSSP-1, Suppressor of CDC2 with RNA-binding motif 2
Ncbi ID
9606, NP_002888, NP_058520, NP_002888.1, NM_016836, NM_002897, NP_001135403.1, NM_001141932, NM_001141931, NM_001012184, NM_016839
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP)
Background of RBMS1 antibody
RBMS1 is a member of a small family of proteins which bind single stranded DNA/RNA. These proteins are characterized by the presence of two sets of ribonucleoprotein consensus sequence (RNP-CS) that contain conserved motifs, RNP1 and RNP2, originally described in RNA binding proteins, and required for DNA binding. These proteins have been implicated in such diverse functions as DNA replication, gene transcription, cell cycle progression and apoptosis.This gene encodes a member of a small family of proteins which bind single stranded DNA/RNA. These proteins are characterized by the presence of two sets of ribonucleoprotein consensus sequence (RNP-CS) that contain conserved motifs, RNP1 and RNP2, originally described in RNA binding proteins, and required for DNA binding. These proteins have been implicated in such diverse functions as DNA replication, gene transcription, cell cycle progression and apoptosis. Multiple transcript variants, resulting from alternative splicing and encoding different isoforms, have been described. Several of these were isolated by virtue of their binding to either strand of an upstream element of c-myc (MSSPs), or by phenotypic complementation of cdc2 and cdc13 mutants of yeast (scr2), or as a potential human repressor of HIV-1 and ILR-2 alpha promoter transcription (YC1). A pseudogene for this locus is found on chromosome 12.
General readings
Niki,T., (2000) FEBS Lett. 475 (3), 209-212
RBMS1 monoclonal antibody (M02), clone M1. Western Blot analysis of RBMS1 expression in SJCRH30 ( Cat # L027V1 ).
RBMS1 monoclonal antibody (M02), clone M1. Western Blot analysis of RBMS1 expression in MES-SA/Dx5 ( Cat # L021V1 ).
RBMS1 monoclonal antibody (M02), clone M1 Western Blot analysis of RBMS1 expression in HeLa ( Cat # L013V1 ).
Human Skin; Rabbit Anti-RBMS1 Antibody. Catalog Number: ARP40427. Paraffin Embedded Tissue: Human Skin. Cellular Data: Squamous epithelial cells. Antibody Concentration: 4.0-8.0 ug/ml. Magnification: 400X; RBMS1 antibody - C-terminal region (ARP40427_T100) in Human Skin cells using Immunohistochemistry
Human Jurkat; WB Suggested Anti-RBMS1 Antibody Titration: 2.5ug/ml. Positive Control: Jurkat cell lysate; RBMS1 antibody - C-terminal region (ARP40427_T100) in Human Jurkat cells using Western Blot
Human HepG2; WB Suggested Anti-RBMS1 Antibody Titration: 1.25ug/ml. Positive Control: HepG2 cell lysate. RBMS1 is strongly supported by BioGPS gene expression data to be expressed in Human HepG2 cells.; RBMS1 antibody - middle region (ARP40428_T100) in Human HepG2 cells using Western Blot
Immunofluorescence of purified MaxPab antibody to RBMS1 on HeLa cell. [antibody concentration 10 ug/ml]
Western Blot analysis of RBMS1 expression in transfected 293T cell line (H00005937-T01) by RBMS1 MaxPab polyclonal antibody.Lane 1: RBMS1 transfected lysate(44.66 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of RBMS1 expression in transfected 293T cell line (H00005937-T03) by RBMS1 MaxPab polyclonal antibody.Lane 1: RBMS1 transfected lysate(44.50 KDa).Lane 2: Non-transfected lysate.
Immunoprecipitation of RBMS1 transfected lysate using anti-RBMS1 MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with RBMS1 MaxPab mouse polyclonal antibody (B01) (H00005937-B01).
Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-RBMS1 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808580)(1:150)
Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-RBMS1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808580)(1:150)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-RBMS1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808580)(1:150)
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-RBMS1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808580)(1:150)
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-RBMS1 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808580)(1:150)
Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-RBMS1 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808580)(1:150)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-RBMS1 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808580)(1:150)
Immunohistochemical staining of paraffin-embedded Human lung tissue within the normal limits using anti-RBMS1 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808580)(1:150)
Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-RBMS1 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808580)(1:150)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-RBMS1 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808580)(1:150)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-RBMS1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808580)(1:150)
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-RBMS1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808580)(1:150)
Immunohistochemical staining of paraffin-embedded Human thyroid tissue within the normal limits using anti-RBMS1 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808580)(1:150)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-RBMS1 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808580)(1:150)
Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-RBMS1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808580)(1:150)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-RBMS1 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808580)(1:150)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-RBMS1 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808580)(1:150)
Immunohistochemical staining of paraffin-embedded Human bladder tissue within the normal limits using anti-RBMS1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808580)(1:150)
Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-RBMS1 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808580)(1:150)
Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-RBMS1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808580)(1:150)
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-RBMS1 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808580)(1:150)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RBMS1 (RC203437, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RBMS1.(1:2000)
Predicted band size: 45 kDa . Positive control: Human liver cancer tissue lysate. Recommended dilution: 1/200-1000. (Gel: 12%SDS-PAGE Lysate: 30 ug per lane Primary antibody: 1/300 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 20 seconds)
Predicted cell location: Nucleus. Positive control: Human colon and ovarian cancer tissue. Recommended dilution: 1/25-100 The image on the left is immunohistochemistry of paraffin-embedded human colon cancer tissue using RBMS1 antibody at dilution 1/30, on the right is treated with the synthetic peptide. (Original magnification:x 200)
Predicted cell location: Nucleus. Positive control: Human colon and ovarian cancer tissue. Recommended dilution: 1/25-100 The image on the left is immunohistochemistry of paraffin-embedded human ovarian cancer tissue using RBMS1 antibody at dilution 1/30, on the right is treated with the synthetic peptide. (Original magnification:x200)
WB Suggested Anti-RBMS1 Antibody Titration: 2.5 ug/ml; Positive Control: Jurkat cell lysate
Rabbit Anti-RBMS1 Antibody; Paraffin Embedded Tissue: Human Skin; Cellular Data: Squamous epithelial cells; Antibody Concentration: 4.0-8.0 ug/ml; Magnification: 400X
WB Suggested Anti-RBMS1 Antibody Titration: 1.25 ug/ml; Positive Control: HepG2 cell lysate; RBMS1 is strongly supported by BioGPS gene expression data to be expressed in Human HepG2 cells
- Quick-Filters:
- Highly Validated (1)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA808580 | RBMS1 antibody |
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| RBMS1 mouse monoclonal antibody, clone OTI2H1 (formerly 2H1) | |||||||
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Mouse | IgG1 | OTI2H1 | Purified | Hu | P, WB |
0.1 ml /
€360.00
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| OriGene Technologies, Inc. | |||||||
 +21 additional images |
Highly validated antibody!
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| TA322935 | RBMS1 antibody |
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| Anti-RBMS1 Rabbit Polyclonal Antibody | |||||||
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Rabbit | IgG | Purified | Hu, Ms, Rt | E, WB |
0.1 ml /
€335.00
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| OriGene Technologies, Inc. | |||||||
| TA322936 | RBMS1 antibody |
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| Anti-RBMS1 Rabbit Polyclonal Antibody | |||||||
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Rabbit | IgG | Purified | Hu, Ms, Rt | E, P |
0.1 ml /
€335.00
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|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP301015 | RBMS1 (transcript variant 3) |
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| Recombinant protein of human RNA binding motif, single stranded interacting protein 1 (RBMS1), transcript variant 3 | |||||
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Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
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| OriGene Technologies, Inc. | |||||
| TP760059 | RBMS1 (full length, N-term HIS tag, transcript variant 1) |
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| Recombinant protein of human RNA binding motif, single stranded interacting protein 1 (RBMS1), transcript variant 1, full length, with N-terminal HIS tag, expressed in E.Coli, 50ug | |||||
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Human | > 80 % Preparation: . Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
E. coli |
50 µg /
€205.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY413821 | RBMS1 overexpression lysate |
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0.1 mg /
€295.00
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| OriGene Technologies, Inc. | ||
| LY419042 | RBMS1 overexpression lysate |
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0.1 mg /
€295.00
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| OriGene Technologies, Inc. |
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR206036L2V | Lenti ORF particles, Rbms1 (GFP-tagged) - Mouse RNA binding motif, single stranded interacting protein 1 (Rbms1), transcript variant 1, 200 uL, >10^7 TU/mL |
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200 µl /
€930.00
|
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| OriGene Technologies, Inc. | ||
| MR216431L2V | Lenti ORF particles, Rbms1 (GFP-tagged) - Mouse RNA binding motif, single stranded interacting protein 1 (Rbms1), transcript variant 2, 200 uL, >10^7 TU/mL |
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200 µl /
€930.00
|
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| OriGene Technologies, Inc. | ||
| MR206036L1V | Lenti ORF particles, Rbms1 (Myc-DDK-tagged) - Mouse RNA binding motif, single stranded interacting protein 1 (Rbms1), transcript variant 1, 200 uL, >10^7 TU/mL |
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200 µl /
€830.00
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| OriGene Technologies, Inc. | ||
| MR216431L1V | Lenti ORF particles, Rbms1 (Myc-DDK-tagged) - Mouse RNA binding motif, single stranded interacting protein 1 (Rbms1), transcript variant 2, 200 uL, >10^7 TU/mL |
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200 µl /
€830.00
|
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| OriGene Technologies, Inc. | ||
Rat Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RR212095L2V | Lenti ORF particles, Rbms1 (GFP-tagged ORF) - Rat RNA binding motif, single stranded interacting protein 1 (Rbms1), 200 uL, >10^7 TU/mL |
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200 µl /
€930.00
|
|
| OriGene Technologies, Inc. | ||
| RR212095L1V | Lenti ORF particles, Rbms1 (Myc-DDK-tagged ORF) - Rat RNA binding motif, single stranded interacting protein 1 (Rbms1), 200 uL, >10^7 TU/mL |
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200 µl /
€830.00
|
|
| OriGene Technologies, Inc. | ||