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RFP-Tag antibody

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Principal name

RFP-Tag antibody

Alternative names for RFP-Tag antibody

Red fluorescent protein Tag, DsRed Tag

SwissProt ID

Q9U6Y8 (Dissp)

Ncbi ID

86600

Available reactivities

Hu (Human), Ms (Mouse)

Available hosts

Rabbit, Mouse

Available applications

Frozen Sections (C), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Immunoprecipitation (IP), Paraffin Sections (P), Flow Cytometry (F), Dot blot (Dot)

Background of RFP-Tag antibody

Epitope tagging is a powerful and versatile strategy for detecting and purifying proteins expressed by cloned genes. To utilize this feature, protein expression vectors are typically engineered with a nucleotide sequence that encodes the peptide epitope tag. The gene of interest is cloned in-frame relative to the tag and, upon expression, the protein of interest is synthesized as a fusion protein with the peptide tag. Fusion protein detection and/or purification is mediated by highly specific antibodies to the engineered peptide, thus eliminating the need for antibodies to proteins from each newly cloned gene. Commonly used epitope tags include glutathione-S-transferase (GST), c-myc, 6-histidine (6X-His), FLAG, green fluorescent protein (GFP), red fluorescent protein (RFP, DSRed), maltose binding protein (MBP), influenza A virus haemagglutinin (HA), b-galactosidase, and GAL4.

PAB11376 - RFP-Tag
Western blot of RFP recombinant protein detected with RFP polyclonal antibody ( Cat # PAB11376 ).Lane 1 shows no reaction against a GFP recombinant protein present in 10 µg of HeLa cell extract.Lane 2 shows a single band detected in 10 µg of a HeLa lysate containing RFP recombinant protein as a 27 kDa band.A 4-12% Bis-Tris gradient gel ( Invitrogen ) was used for SDS-PAGE. The membrane was blocked and then probed with RFP polyclonal antibody ( Cat # PAB11376 ) diluted 1:2,500 for 1 h at RT.
PAB9104 - RFP-Tag
Western blot analysis with recombinant RFP.Lane 1 : 10 ng.Lane 2 : 50 ng.Lane 3 : 250 ng.Lane 4 : 1 µg.
AP09229PU-N - RFP-Tag
Western blot of RFP recombinant protein detected with polyclonal anti-RFP antibody. Lane 1 shows no reaction against a GFP recombinant protein present in 10 μg of HeLa cell extract. Lane 2 shows a single band detected in 10 μg of a HeLa lysate containing RFP recombinant protein as a 27 kDa band. A 4-12% Bis-Tris gradient gel was used for SDS-PAGE. The membrane was blocked and then probed with Anti-RFP diluted 1:2,500 for 1 h at RT followed by washes and reaction with a 1:5,000 dilution of IRDye(TM)800 conjugated Goat-a-Rabbit IgG [H&L]. IRDye(TM)800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.
AP09229FC-N - RFP-Tag
Western blot of RFP recombinant protein detected withanti-RFP antibody. Lane 1 shows no reaction against a GFP recombinant protein present in 10 μg of HeLa cell extract. Lane 2 shows a single band detected in 10 μg of a HeLa lysate containing RFP recombinant protein. Anti-RFP detects a 27 kDa band corresponding to the epitope tag RFP. A 4-12% Bis-Tris gradient gel (Invitrogen) was used for SDSPAGE. The protein was transferred to nitrocellulose using standard methods. After blocking the membrane was probed with the primary antibody diluted 1:2,500 for 1 h at room temperature followed by washes and reaction with a 1:5,000 dilution of IRDye(TM)800 conjugated Goat-a-Rabbit IgG [H&L]. IRDye(TM)800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.
AP09229HR-N - RFP-Tag
Western blot of RFP recombinant protein detected with anti-RFP antibody. Lane 1 shows no reaction against a GFP recombinant protein present in 10 μg of HeLa cell extract. Lane 2 shows a single band detected in 10 μg of a HeLa lysate containing RFP recombinant protein. Anti-RFP detects a 27 kDa band corresponding to the epitope tag RFP. A 4-12% Bis-Tris gradient gel (Invitrogen) was used for SDS-PAGE. The protein was transferred to nitrocellulose using standard methods. After blocking the membrane was probed with the primary antibody diluted 1:2,500 for 1 h at room temperature followed by washes and reaction with a 1:5,000 dilution of IRDye(TM)800 conjugated Goat-a-Rabbit IgG [H&L]. IRDye(TM)800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.
AP09229BT-N - RFP-Tag
Western blot of RFP recombinant protein detected with anti-RFP antibody. Lane 1 shows no reaction against a GFP recombinant protein present in 10 μg of HeLa cell extract. Lane 2 shows a single band detected in 10 μg of a HeLa lysate containing RFP recombinant protein. Separation was achieved using a 4-12% Bis Tris gradient gel followed by transfer to nitrocellulose and blocking.The membrane was probed with the primary antibody diluted 1:2,500 for 1 h at room temperature followed by washes and reaction with a 1:5,000 dilution of IRDye(TM)800 conjugated Goat-a-Rabbit IgG [H&L]. IRDye(TM)800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.
AM20837PU-N - RFP-Tag
RFP Monoclonal Antibodydilution at 1/100 (1 µg/ml) probed against HEK293 cells transfected with RFP-tagged protein vector. Lane 1: Untransfected Control. Lane 2: Transfected with Turbo-RFP. Lane 3: Transfected with DeRed
AM26650AF-N - RFP-Tag
Immunocytochemical detection of mRFP1 on 4% PFA fixed transfectant* using AM26650AF-N (left). Right panel is mRFP1 own flourescence.
*This transfectant is provided by RIKEN.
AM26650AF-N - RFP-Tag
Immunocytochemical detection of DsRed on 4% PFA fixed transfectant using AM26650AF-N (left). Right panel is DsRed own fluorescence.
AM26650AF-N - RFP-Tag
Western blot analysis of DsRed(1), mRFP1* (2), mCherry* (3), mOrange* (4) and mPlum* (5) using AM26650AF-N. *Sample number (2) to (5) are provided by RIKEN.
AM26655AF-N - RFP-Tag
Immunhoprecipitation of DsRed(1, 2), mRFP1* (3), mCherry* (4), mOrange* (5) and mPlum* (6) with isotype control (1) or AM26655AF-N (2-6). After immunoprecipitatedwith the antibody, immunocomplexwas resolved on SDS-PAGE and stained with CBB.*Sample number (3) to (6) are provided by RIKEN.
AM26655AF-N - RFP-Tag
chainRFPImmunoprecipitation of DsRed fusion protein (1, 2), mRFP1* (3) and mCherry* (4) with mouse IgG1 (1) or AM26655AF-N (2-4). After immunoprecipitatedwith the antibody,immunocomplexwas resolved on SDS-PAGE and immunoblottedwith anti-RFP monoclonal antibody. *Sample number (3) and (4) are provided by RIKEN.
AM26655AG-N - RFP-Tag

Immunoprecipitation of DsRed (1, 2), mRFP1* (3), mCherry* (4), mOrange* (5) and mPlum* (6) with isotype control (1) or AM26655AG-N (2-6). After immunoprecipitated with the antibody, immunocomplex was resolved on SDS-PAGE and stained with CBB. *Sample number (3) to (6) are provided by RIKEN.


AM26655AG-N - RFP-Tag

Immunoprecipitation of DsRed (1, 2), mRFP1* (3) and mCherry* (4) with isotype control (1) or AM26655AG-N (2-4). After immunoprecipitated with the antibody, immunocomplex was resolved on SDS-PAGE and immunoblotted with anti-RFP monoclonal antibody. *Sample number (3) and (4) are provided by RIKEN.


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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

RFP-Tag (Ads. to Hu, Ms, Rt Serum Proteins) antibody
Western blot of RFP recombinant protein detected with polyclonal anti-RFP antibody. Lane 1 shows no reaction against a GFP recombinant protein present in 10 μg of HeLa cell extract. Lane 2 shows a single band detected in 10 μg of a HeLa lysate containing RFP recombinant protein as a 27 kDa band. A 4-12% Bis-Tris gradient gel was used for SDS-PAGE. The membrane was blocked and then probed with Anti-RFP diluted 1:2,500 for 1 h at RT followed by washes and reaction with a 1:5,000 dilution of IRDye(TM)800 conjugated Goat-a-Rabbit IgG [H&L]. IRDye(TM)800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results. Rabbit IgG Aff - Purified E, P, WB
0.1 mg / €350.00
  OriGene Technologies GmbH
+4 additional images

Published recently!

Highly validated antibody!

RFP-Tag antibody
RFP Monoclonal Antibody dilution at 1/100 (1 µg/ml) probed against HEK293 cells transfected with RFP-tagged protein vector. Lane 1: Untransfected Control. Lane 2: Transfected with Turbo-RFP. Lane 3: Transfected with DeRed Mouse IgG1 RFP. H8 Aff - Purified Dot, IP, WB
0.1 mg / €360.00
  OriGene Technologies GmbH

RFP-Tag antibody
Immunocytochemical detection of mRFP1 on 4% PFA fixed transfectant* using AM26650AF-N (left). Right panel is mRFP1 own flourescence.*This transfectant is provided by RIKEN. Mouse IgG1 8D6 Azide Free ICC/IF, WB
0.1 ml / €430.00
  OriGene Technologies GmbH
+2 additional images

RFP-Tag antibody
Immunhoprecipitation of DsRed(1, 2), mRFP1* (3), mCherry* (4), mOrange* (5) and mPlum* (6) with isotype control (1) or AM26655AF-N (2-6). After immunoprecipitatedwith the antibody, immunocomplexwas resolved on SDS-PAGE and stained with CBB.*Sample number (3) to (6) are provided by RIKEN. Mouse IgG2a 3G5 Azide Free IP
0.1 mg / €340.00
  OriGene Technologies GmbH
+1 additional image

RFP-Tag antibody
Immunoprecipitation of DsRed (1, 2), mRFP1* (3), mCherry* (4), mOrange* (5) and mPlum* (6) with isotype control (1) or AM26655AG-N (2-6). After immunoprecipitated with the antibody, immunocomplex was resolved on SDS-PAGE and stained with CBB. *Sample number (3) to (6) are provided by RIKEN. Mouse IgG2a 3G5 Agarose IP
0.2 ml / €375.00
  OriGene Technologies GmbH
+1 additional image

RFP-Tag (Ads. to Hu, Ms, Rt Serum Proteins) antibody
Western blot of RFP recombinant protein detected with anti-RFP antibody. Lane 1 shows no reaction against a GFP recombinant protein present in 10 μg of HeLa cell extract. Lane 2 shows a single band detected in 10 μg of a HeLa lysate containing RFP recombinant protein. Separation was achieved using a 4-12% Bis Tris gradient gel followed by transfer to nitrocellulose and blocking.The membrane was probed with the primary antibody diluted 1:2,500 for 1 h at room temperature followed by washes and reaction with a 1:5,000 dilution of IRDye(TM)800 conjugated Goat-a-Rabbit IgG [H&L]. IRDye(TM)800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results. Rabbit IgG Biotin C, E, WB
0.1 mg / €515.00
  OriGene Technologies GmbH

RFP-Tag (Ads. to Hu, Ms, Rt Serum Proteins) antibody
Western blot of RFP recombinant protein detected withanti-RFP antibody. Lane 1 shows no reaction against a GFP recombinant protein present in 10 μg of HeLa cell extract. Lane 2 shows a single band detected in 10 μg of a HeLa lysate containing RFP recombinant protein. Anti-RFP detects a 27 kDa band corresponding to the epitope tag RFP. A 4-12% Bis-Tris gradient gel (Invitrogen) was used for SDSPAGE. The protein was transferred to nitrocellulose using standard methods. After blocking the membrane was probed with the primary antibody diluted 1:2,500 for 1 h at room temperature followed by washes and reaction with a 1:5,000 dilution of IRDye(TM)800 conjugated Goat-a-Rabbit IgG [H&L]. IRDye(TM)800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results. Rabbit IgG FITC E, F, ICC/IF, WB
0.1 mg / €515.00
  OriGene Technologies GmbH

RFP-Tag (Ads. to Hu, Ms, Rt Serum Proteins) antibody
Western blot of RFP recombinant protein detected with anti-RFP antibody. Lane 1 shows no reaction against a GFP recombinant protein present in 10 μg of HeLa cell extract. Lane 2 shows a single band detected in 10 μg of a HeLa lysate containing RFP recombinant protein. Anti-RFP detects a 27 kDa band corresponding to the epitope tag RFP. A 4-12% Bis-Tris gradient gel (Invitrogen) was used for SDS-PAGE. The protein was transferred to nitrocellulose using standard methods. After blocking the membrane was probed with the primary antibody diluted 1:2,500 for 1 h at room temperature followed by washes and reaction with a 1:5,000 dilution of IRDye(TM)800 conjugated Goat-a-Rabbit IgG [H&L]. IRDye(TM)800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results. Rabbit IgG HRP C, E, WB
0.1 mg / €515.00
  OriGene Technologies GmbH

8 Item(s)

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Product Type
  1. Primary Antibody (8)
Clonality
  1. Monoclonal (4)
  2. Polyclonal (4)
Target
  1. RFP-Tag (8)
Property
  1. Ads. to Hu, Ms, Rt Serum Proteins (4)
Applications
  1. Western blot / Immunoblot (WB) (6)
  2. Enzyme Immunoassay (E) (4)
  3. Immunoprecipitation (IP) (3)
  4. Frozen sections (C) (2)
  5. Immunocytochemistry/​Immunofluorescence (ICC/IF) (2)
  6. Dot blot (Dot) (1)
  7. Flow cytometry (F) (1)
  8. Paraffin Sections (P) (1)
Presentation
  1. Aff - Purified (2)
  2. Azide Free (2)
  3. Agarose (1)
  4. Biotin (1)
  5. FITC (1)
  6. HRP (1)
Host
  1. Mouse (4)
  2. Rabbit (4)
Images
  1. 1 image (8)
  2. 3+ images (2)
  3. 5+ images (1)
Images: Applications
  1. Western blot / Immunoblot (WB) (6)
  2. Immunocytochemistry/​Immunofluorescence (ICC/IF) (2)
  3. Immunoprecipitation (IP) (2)
Manufacturer
  1. OriGene Technologies (8)
Service
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