RhoA antibody
Principal name
RhoA antibody
Alternative names for RhoA antibody
ARH12, ARHA, RHO12, Transforming protein RhoA, Rho cDNA clone 12
Ncbi ID
9606, NP_001655.1, NP_001655, NP_058082.2, NM_016802, NM_057132
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), ELISA (detection) (E(detection))
Background of RhoA antibody
Regulates a sigl transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. Involved in a microtubule-dependent sigl that is required for the myosin contractile ring formation during cell cycle cytokinesis. Plays an essential role in cleavage furrow formation. Required for the apical junction formation of keratinocyte cell-cell adhesion. Serves as a target for the yopT cysteine peptidase from Yersinia pestis; vector of the plague; and Yersinia pseudotuberculosis; which causes gastrointestil disorders. Stimulates PKN2 kise activity. May be an activator of PLCE1. Activated by ARHGEF2; which promotes the exchange of GDP for GTP. Essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly. The MEMO1-RHOA-DIAPH1 sigling pathway plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex. It controls the localization of APC and CLASP2 to the cell membrane; via the regulation of GSK3B activity. In turn; membrane-bound APC allows the localization of the MACF1 to the cell membrane; which is required for microtubule capture and stabilization.
Detection limit for recombinant GST tagged RHOA is approximately 3ng/ml as a capture antibody.
RHOA monoclonal antibody (M03), clone 1A11 Western Blot analysis of RHOA expression in HeLa ( Cat # L013V1 ).
Detection limit for recombinant GST tagged RHOA is approximately 3ng/ml as a capture antibody.
Immunoperoxidase of monoclonal antibody to RHOA on formalin-fixed paraffin-embedded human lymphoma tissue.[antibody concentration 5ug/ml]
Immunofluorescence of monoclonal antibody to RHOA on HeLa cell. [antibody concentration 10 ug/ml]
RHOA monoclonal antibody (M04), clone 1B12 Western Blot analysis of RHOA expression in HL-60 ( Cat # L014V1 ).
Detection limit for recombinant GST tagged RHOA is approximately 10ng/ml as a capture antibody.
Immunoperoxidase of monoclonal antibody to RHOA on formalin-fixed paraffin-embedded human tonsil tissue.[antibody concentration 5ug/ml]
Proximity Ligation Analysis (PLA) of protein-protein interactions between MAPK8 and RHOA HeLa cells were stained with anti-MAPK8 rabbit purified polyclonal 1:1200 and anti-RHOA mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.
RHOA monoclonal antibody (M05), clone 1C1 Western Blot analysis of RHOA expression in HeLa ( Cat # L013V1 ).
Western Blot analysis of RHOA expression in transfected 293T cell line by RHOA monoclonal antibody (M05), clone 1C1.Lane 1: RHOA transfected lysate(21.8 KDa).Lane 2: Non-transfected lysate.
Detection limit for recombinant GST tagged RHOA is 3 ng/ml as a capture antibody.
Immunofluorescence of monoclonal antibody to RHOA on A-431 cell. [antibody concentration 10 ug/ml]
RHOA monoclonal antibody (M06), clone 1C10 Western Blot analysis of RHOA expression in A-431 ( Cat # L015V1 ).
Western Blot analysis of RHOA expression in transfected 293T cell line by RHOA monoclonal antibody (M07), clone 1C11.Lane 1: RHOA transfected lysate(21.8 KDa).Lane 2: Non-transfected lysate.
RHOA monoclonal antibody (M08), clone 1C3 Western Blot analysis of RHOA expression in A-431 ( Cat # L015V1 ).
Detection limit for recombinant GST tagged RHOA is approximately 3ng/ml as a capture antibody.
Immunohistochemistry (IHC) analyzes of Rho A antibody (Cat.-No.: AP06618PU-N) in paraffin-embedded human breast carcinoma tissue.
Western blot (WB) analysis of Rho A antibody (Cat.-No.: AP06618PU-N) in extracts from HepG2 cells.
AP15760PU antibody staining of MCF7 cell lysate by Western Blot.
Proximity Ligation Analysis (PLA) of protein-protein interactions between RHOA and DAAM1 HeLa cells were stained with anti-RHOA rabbit purified polyclonal 1:1200 and anti-DAAM1 mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.
Western Blot analysis of RHOA expression in transfected 293T cell line (H00000387-T02) by RHOA MaxPab polyclonal antibody.Lane 1: RHOA transfected lysate(21.80 KDa).Lane 2: Non-transfected lysate.
293T; WB Suggested Anti-RHOA Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: 293T cell lysate; RHOA antibody - middle region (ARP58521_P050) in 293T cells using Western Blot
Recombinant human RhoA, 1-190 aa, His-tagged
Human Breast: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Small Intestine, Myenteric Plexus: Formalin-Fixed, Paraffin-Embedded (FFPE)
Immunofluorescence of monoclonal antibody to RHOA on HeLa cell at 10 µg/ml.
Western Blot analysis of RHOA expression in HL-60 cell lysate.
Western Blot analysis of RHOA expression in transfected 293T cell line. Lane 1: RHOA transfected lysate(21.8 KDa). Lane 2: Non-transfected lysate.
Detection limit for recombinant GST tagged RHOA is approximately 10ng/ml as a capture antibody.
Human Breast: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Small Intestine, Myenteric Plexus: Formalin-Fixed, Paraffin-Embedded (FFPE)
Western Blot analysis of RHOA expression in transfected 293T cell line. Lane 1: RHOA transfected lysate(21.8 KDa). Lane 2: Non-transfected lysate
Western Blot analysis of RHOA expression in HeLa cell lysate.
Detection limit for recombinant GST tagged RHOA is 3 ng/ml as a capture antibody.
Predicted band size: 22 kDa . Positive control: NIH/3T3 cell and Mouse brain tissue lysate. Recommended dilution: 1/200-1000. (Gel: 12%SDS-PAGE Lane1: NIH/3T3 cell tissue lysate Lane1: Mouse brain tissue lysate Lysates: 40 ug per lane Primary antibody: 1/200 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute)
Predicted band size: 22 kDa . Positive control: K562, 231 and Hela cell, Human fetal brain tissue lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lane1: K562 cell lysate Lane2: 231 cell lysate Lane3: Hela cell lysate Lane4: Human fetal brain tissue lysate Lysates: 40 ug per lane Primary antibody: 1/450 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 3 minutes)
Predicted band size: 22 kDa . Positive control: K562, 231 and Hela cell, Human fetal brain tissue lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lane1: K562 cell lysate Lane2: 231 cell lysate Lane3: Hela cell lysate Lane4: Human fetal brain tissue lysate Lysates: 40 ug per lane Primary antibody: 1/400 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 3 minutes)
Western blot analysis of RhoA expression in HeLa whole cell lysates,The lane on the left is treated with the antigen-specific peptide.
WB Suggested Anti-RHOA Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:62500; Positive Control: 293T cell lysate
Western blot analysis of RhoA phosphorylation expression in HeLa whole cell lysates,The lane on the left is treated with the antigen-specific peptide.
- Quick-Filters:
- Highly Validated (2)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| AM31718PU-N | RhoA antibody |
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 |
Mouse | IgG1 | 1B12 | Purified | Hu | E, ICC/IF, P, WB |
50 µg /
€475.00
|
| OriGene Technologies GmbH | |||||||
 +5 additional images |
Highly validated antibody! |
||||||
| AM31719PU-N | RhoA antibody |
||||||
 |
Mouse | IgG1 | 1C1 | Purified | Hu | E, P, WB |
50 µg /
€475.00
|
| OriGene Technologies GmbH | |||||||
| +4 additional images |
Highly validated antibody! |
||||||
| TA321392 | RhoA antibody |
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| Anti-RHOA Rabbit Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA321393 | RhoA antibody |
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| Anti-RHOA Rabbit Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA321394 | RhoA antibody |
||||||
| Anti-RHOA Rabbit Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA325812 | RhoA antibody |
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| Rabbit Polyclonal RhoA Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA325813 | RhoA antibody |
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| Rabbit Polyclonal RhoA (Ser188) Antibody (Phospho-specific) | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| AP06618PU-N | RhoA antibody |
||||||
 |
Rabbit | Aff - Purified | Hu, Ms | P, WB |
0.1 mg /
€370.00
|
||
| OriGene Technologies GmbH | |||||||
| +1 additional image |
|||||||
CRISPR
| Catalog No. | Species | Donor Vector | |
|---|---|---|---|
| KN203303 | RHOA - human gene knockout kit via CRISPR |
||
 |
Human | GFP-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN203303BN | RHOA - human gene knockout kit via CRISPR |
||
|
Human | mBFP-Neo |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN203303LP | RHOA - human gene knockout kit via CRISPR |
||
|
Human | Luciferase-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN203303RB | RHOA - human gene knockout kit via CRISPR |
||
|
Human | RFP-BSD |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP303303 | RhoA |
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| Recombinant protein of human ras homolog gene family, member A (RHOA) | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR201870L2V | Lenti ORF particles, Rhoa (GFP-tagged) - Mouse ras homolog gene family, member A (Rhoa), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€930.00
|
|
| OriGene Technologies, Inc. | ||
| MR201870L1V | Lenti ORF particles, Rhoa (Myc-DDK-tagged) - Mouse ras homolog gene family, member A (Rhoa), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€830.00
|
|
| OriGene Technologies, Inc. | ||
Rat Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RR215409L2V | Lenti ORF particles, Rhoa (GFP-tagged ORF) - Rat ras homolog gene family, member A (Rhoa), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€930.00
|
|
| OriGene Technologies, Inc. | ||
| RR215409L1V | Lenti ORF particles, Rhoa (Myc-DDK-tagged ORF) - Rat ras homolog gene family, member A (Rhoa), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€830.00
|
|
| OriGene Technologies, Inc. | ||