RhoB antibody
Principal name
RhoB antibody
Alternative names for RhoB antibody
ARH6, ARHB, Rho-related GTP-binding protein RhoB, Rho cDNA clone 6
Ncbi ID
Available reactivities
Available hosts
Available applications
ELISA (detection) (E(detection)), Western blot / Immunoblot (WB), Enzyme Immunoassay (E)
Background of RhoB antibody
- Kit Component:
- - KN209837G1, RHOB gRNA vector 1 in pCas-Guide vector
- KN209837G2, RHOB gRNA vector 2 in pCas-Guide vector
- KN209837D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Wennerberg K., et al. (2004) Journal of cell science 117 (Pt 8): 1301–12.
Wherlock M., et al (2004) J. Cell Sci. 117:3221-3231.
Western Blot analysis of RHOB expression in transfected 293T cell line (H00000388-T02) by RHOB MaxPab polyclonal antibody.Lane 1: RHOB transfected lysate(22.10 KDa).Lane 2: Non-transfected lysate.
Recombinant human RhoB, 1-193aa, His-tagged
ARHB Antibody (Center) (Cat. #AP50230PU-N)immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ARHB Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
ARHB Antibody (Center) (Cat. #AP50230PU-N) western blot analysis in HL-60 cell line lysates (35µg/lane).This demonstrates the ARHB antibody detected the ARHB protein (arrow).
Western blot analysis of extracts from HepG2 cells and HeLa cells, using RHOB antibody.The lane on the right is treated with the synthesized peptide.
Gel: 10%SDS-PAGE, Lysate: 40 µg, Lane 1-2: Mouse brain tissue, Mouse fetal brain tissue, Primary antibody: RHOB Antibody at dilution 1/800, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 15 seconds
WB Suggested Anti-RHOB Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 1562500; Positive Control: Human Placenta
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA313472 | RhoB antibody |
||||||
| Rabbit polyclonal anti-RHOB antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms | E, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA322808 | RhoB antibody |
||||||
| Anti-RHOB Rabbit Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| AP50230PU-N | RhoB (Center) antibody |
||||||
 |
Rabbit | Ig | Purified | Hu | WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies GmbH | |||||||
 +1 additional image |
|||||||
CRISPR
| Catalog No. | Species | Donor Vector | |
|---|---|---|---|
| KN209837 | RHOB - human gene knockout kit via CRISPR |
||
 |
Human | GFP-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN209837BN | RHOB - human gene knockout kit via CRISPR |
||
|
Human | mBFP-Neo |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN209837LP | RHOB - human gene knockout kit via CRISPR |
||
|
Human | Luciferase-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN209837RB | RHOB - human gene knockout kit via CRISPR |
||
|
Human | RFP-BSD |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP309837 | RhoB |
||||
| Recombinant protein of human ras homolog gene family, member B (RHOB) | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR201939L2V | Lenti ORF particles, Rhob (GFP-tagged) - Mouse ras homolog gene family, member B (Rhob), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€930.00
|
|
| OriGene Technologies, Inc. | ||
| MR201939L1V | Lenti ORF particles, Rhob (Myc-DDK-tagged) - Mouse ras homolog gene family, member B (Rhob), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€830.00
|
|
| OriGene Technologies, Inc. | ||